Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma

Autores
Crescenti, Ernesto, J. V.; Medina, Vanina Araceli; Sambuco, Lorena; Cremaschi, Graciela A.; Genaro, Ana María; Cricco, Graciela P.; Martín, Gabriela A.; Valli, Eduardo; Martinel Lamas, Diego José; Perazzo, Juan C.; Rivera, Elena S.; Bergoc, Rosa M.
Año de publicación
2014
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Crescenti, Ernesto, J. V. Institute of Immunooncology; Argentina
Fil: Sambuco, Lorena. Institute of Immunooncology; Argentina
Fil: Medina, Vanina A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cricco, Graciela P. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Martín, Gabriela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Rivera, Elena S. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Bergoc, Rosa M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Genaro, Ana María. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Genaro, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martín, Gabriela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Vanina A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perazzo, Juan C. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Patofisiología; Argentina
Abstract: Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P < 0.01), collagen deposition, and mast cells infiltration (43.1 ± 1.0 vs. 102 ± 14.1 mast cells, P < 0.05). O-LM administration significantly blocked BLM-induced oxidative damage and the enhanced immunoreactive fibroblasts for α-smooth muscle actin while reduced BLM-induced autoantibodies that strongly react mainly with skin and spleen. O-LM significantly reduced BLM-induced scleroderma through the modulation of antioxidant and immunological pathways.
Fuente
Biological Trace Element Research. 2014, 157
Materia
ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/14264
Acceder
id RIUCA_02aa71f394e23897f318fab76f64724a
oai_identifier_str oai:ucacris:123456789/14264
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental sclerodermaCrescenti, Ernesto, J. V.Medina, Vanina AraceliSambuco, LorenaCremaschi, Graciela A.Genaro, Ana MaríaCricco, Graciela P.Martín, Gabriela A.Valli, EduardoMartinel Lamas, Diego JoséPerazzo, Juan C.Rivera, Elena S.Bergoc, Rosa M.ESCLERODERMIA SISTEMICAANTICUERPOSANTIOXIDANTESMASTOCITOSMANGANESIOSELENIOPIELZINCFil: Crescenti, Ernesto, J. V. Institute of Immunooncology; ArgentinaFil: Sambuco, Lorena. Institute of Immunooncology; ArgentinaFil: Medina, Vanina A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Cricco, Graciela P. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Martín, Gabriela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Rivera, Elena S. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Bergoc, Rosa M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; ArgentinaFil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; ArgentinaFil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; ArgentinaFil: Genaro, Ana María. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; ArgentinaFil: Genaro, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martín, Gabriela A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Medina, Vanina A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perazzo, Juan C. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Patofisiología; ArgentinaAbstract: Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P < 0.01), collagen deposition, and mast cells infiltration (43.1 ± 1.0 vs. 102 ± 14.1 mast cells, P < 0.05). O-LM administration significantly blocked BLM-induced oxidative damage and the enhanced immunoreactive fibroblasts for α-smooth muscle actin while reduced BLM-induced autoantibodies that strongly react mainly with skin and spleen. O-LM significantly reduced BLM-induced scleroderma through the modulation of antioxidant and immunological pathways.Springer2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/142641559-0720 (online)0163-498410.1007/s12011-013-9876-424363240Crescenti, E.J.V., et al. Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma [en línea]. Biological Trace Element Research. 2014, 157 doi:10.1007/s12011-013-9876-4 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14264Biological Trace Element Research. 2014, 157reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaspainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:58:38Zoai:ucacris:123456789/14264instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:58:38.437Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
spellingShingle Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
Crescenti, Ernesto, J. V.
ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
title_short Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_full Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_fullStr Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_full_unstemmed Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
title_sort Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma
dc.creator.none.fl_str_mv Crescenti, Ernesto, J. V.
Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
author Crescenti, Ernesto, J. V.
author_facet Crescenti, Ernesto, J. V.
Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
author_role author
author2 Medina, Vanina Araceli
Sambuco, Lorena
Cremaschi, Graciela A.
Genaro, Ana María
Cricco, Graciela P.
Martín, Gabriela A.
Valli, Eduardo
Martinel Lamas, Diego José
Perazzo, Juan C.
Rivera, Elena S.
Bergoc, Rosa M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
topic ESCLERODERMIA SISTEMICA
ANTICUERPOS
ANTIOXIDANTES
MASTOCITOS
MANGANESIO
SELENIO
PIEL
ZINC
dc.description.none.fl_txt_mv Fil: Crescenti, Ernesto, J. V. Institute of Immunooncology; Argentina
Fil: Sambuco, Lorena. Institute of Immunooncology; Argentina
Fil: Medina, Vanina A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cricco, Graciela P. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Martín, Gabriela A. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Martinel Lamas, Diego José. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Rivera, Elena S. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Bergoc, Rosa M. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Laboratorio de Radioisotopos; Argentina
Fil: Cremaschi, Graciela A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Valli, Eduardo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Genaro, Ana María. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neuroinmunomodulación y Oncología Molecular; Argentina; Argentina
Fil: Genaro, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Valli, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martín, Gabriela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Medina, Vanina A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cremaschi, Graciela A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perazzo, Juan C. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Patofisiología; Argentina
Abstract: Scleroderma, sclerosis of the skin, is a severe autoimmune disease refractant to all kind of treatments. To study the in vivo effects of a combination of three oligoelements selenium (Se), zinc (Zn), and manganese (Mn) plus Lachesis muta venom (O-LM) on the bleomycin (BLM)-induced scleroderma mouse experimental model. C3H mice were randomly divided into four groups: control (phosphate-buffered saline (PBS)), O-LM, BLM, and BLM + O-LM. All administrations were performed subcutaneously into the back of mice. BLM was injected 5 days per week for three consecutive weeks and O-LM was administered simultaneously with BLM from the beginning of the experiments and lasted for 3 weeks after the final BLM or PBS injection (for O-LM and BLM + O-LM groups), when animals were sacrificed and histopathological, immunohistochemical, thiobarbituric acid reactive species (TBARS) evaluation, and autoantibodies detection were determined. O-LM significantly reduced BLM-induced enhanced dermal thickness (605 ± 47 vs. 956 ± 59 μm, P < 0.01), collagen deposition, and mast cells infiltration (43.1 ± 1.0 vs. 102 ± 14.1 mast cells, P < 0.05). O-LM administration significantly blocked BLM-induced oxidative damage and the enhanced immunoreactive fibroblasts for α-smooth muscle actin while reduced BLM-induced autoantibodies that strongly react mainly with skin and spleen. O-LM significantly reduced BLM-induced scleroderma through the modulation of antioxidant and immunological pathways.
description Fil: Crescenti, Ernesto, J. V. Institute of Immunooncology; Argentina
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/14264
1559-0720 (online)
0163-4984
10.1007/s12011-013-9876-4
24363240
Crescenti, E.J.V., et al. Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma [en línea]. Biological Trace Element Research. 2014, 157 doi:10.1007/s12011-013-9876-4 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14264
url https://repositorio.uca.edu.ar/handle/123456789/14264
identifier_str_mv 1559-0720 (online)
0163-4984
10.1007/s12011-013-9876-4
24363240
Crescenti, E.J.V., et al. Effects of oligoelements Se, Zn, and Mn plus Lachesis muta venom in experimental scleroderma [en línea]. Biological Trace Element Research. 2014, 157 doi:10.1007/s12011-013-9876-4 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/14264
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Biological Trace Element Research. 2014, 157
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.13397

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