Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

• Autores: Sadañoski, Marcela Alejandra; Benítez, Silvana Florencia; Fonseca, María Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; Levin, Laura Noemí; Villalba, Laura Lidia
• Año de publicación: 2019
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Polychlorinated; Biphenyls; Mycoremediation; Laccase; Morphology; Tolerance
• Institución: Universidad Nacional de Misiones

Fil: Sadañoski, Marcela Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Benítez, Silvana Florencia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Levin, Laura Noemí. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Micología Experimental. Instituto de Micología y Botánica; Argentina.
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Micología y Botánica; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Química y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Polychlorinated biphenyls (PCBs) are exceptionally stable organic pollutants. An effective and economically feasible process is essential for their removal. Mycoremediation using white-rot fungal strains proved as a highly effective approach that has not been thoroughly investigated. The aim of this work was to evaluate PCBs re-mediation efficacy applying Pleurotus sajor-caju LBM 105. Axenic cultures of the fungus, either in complex or N-limited liquid media, were spiked with 217 mg L−1 of a technical mixture of Aroclor 1242, 1254 y 1260 in oil transformer. PCBs removal capacity, toxicity reduction, changes in fungal morphology and laccase expression caused by pollutant addition and the cost-effectiveness of the remediation treatment were assessed when growing the fungus under two different nutritional conditions. P. sajor-caju LBM 105 could remove 97.7% and 91.7% of the PCBs mixture and reduced the toxicity in complex and mineral media respectively after 35 days of incubation. The addition of PCBs to P. sajor-caju LBM 105 culture media resulted in an increase in hyphal diameter, changes in biomass, glucose consumption, proteins and laccase secretion. A 3-fold rise in laccase activity was detected in both media at 28 day. Laccase mRNA augmented 16% and 91% at 21 and 28 days respectively when PCBs were added to the N-limited liquid media. Mycoremediation of PCBs with P. sajor-caju LBM 105 cultivated in N-limited liquid medium is an effective low-cost (0.69 $ g-1d-1) alternative technique for PCBs treatment.