Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment

Autores
Sadañoski, Marcela Alejandra; Benitez, Silvana Florencia; Fonseca, Maria Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; Levin, Laura Noemí; Villalba, Laura Lidia
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Polychlorinated biphenyls (PCBs) are exceptionally stable organic pollutants. An effective and economically feasible process is essential for their removal. Mycoremediation using white-rot fungal strains proved as a highly effective approach that has not been thoroughly investigated. The aim of this work was to evaluate PCBs remediation efficacy applying Pleurotus sajor-caju LBM 105. Axenic cultures of the fungus, either in complex or N-limited liquid media, were spiked with 217 mg L-1 of a technical mixture of Aroclor 1242, 1254 y 1260 in oil transformer. PCBs removal capacity, toxicity reduction, changes in fungal morphology and laccase expression caused by pollutant addition and the cost-effectiveness of the remediation treatment were assessed when growing the fungus under two different nutritional conditions. P. sajor-caju LBM 105 could remove 97.7% and 91.7% of the PCBs mixture and reduced the toxicity in complex and mineral media respectively after 35 days of incubation. The addition of PCBs to P. sajor-caju LBM 105 culture media resulted in an increase in hyphal diameter, changes in biomass, glucose consumption, proteins and laccase secretion. A 3-fold rise in laccase activity was detected in both media at 28 day. Laccase mRNA augmented 16% and 91% at 21 and 28 days respectively when PCBs were added to the N-limited liquid media. Mycoremediation of PCBs with P. sajor-caju LBM 105 cultivated in N-limited liquid medium is an effective low-cost (0.69 $ g-1d-1) alternative technique for PCBs treatment.
Fil: Sadañoski, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Benitez, Silvana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Materia
POLYCHLORINATE BIPHENYLS
MYCOREMEDIATION
LACCASE
MORPHOLOGY
TOLERANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107329
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/107329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatmentSadañoski, Marcela AlejandraBenitez, Silvana FlorenciaFonseca, Maria IsabelVelázquez, Juan ErnestoZapata, Pedro DaríoLevin, Laura NoemíVillalba, Laura LidiaPOLYCHLORINATE BIPHENYLSMYCOREMEDIATIONLACCASEMORPHOLOGYTOLERANCEhttps://purl.org/becyt/ford/1.5https://purl.org/becyt/ford/1Polychlorinated biphenyls (PCBs) are exceptionally stable organic pollutants. An effective and economically feasible process is essential for their removal. Mycoremediation using white-rot fungal strains proved as a highly effective approach that has not been thoroughly investigated. The aim of this work was to evaluate PCBs remediation efficacy applying Pleurotus sajor-caju LBM 105. Axenic cultures of the fungus, either in complex or N-limited liquid media, were spiked with 217 mg L-1 of a technical mixture of Aroclor 1242, 1254 y 1260 in oil transformer. PCBs removal capacity, toxicity reduction, changes in fungal morphology and laccase expression caused by pollutant addition and the cost-effectiveness of the remediation treatment were assessed when growing the fungus under two different nutritional conditions. P. sajor-caju LBM 105 could remove 97.7% and 91.7% of the PCBs mixture and reduced the toxicity in complex and mineral media respectively after 35 days of incubation. The addition of PCBs to P. sajor-caju LBM 105 culture media resulted in an increase in hyphal diameter, changes in biomass, glucose consumption, proteins and laccase secretion. A 3-fold rise in laccase activity was detected in both media at 28 day. Laccase mRNA augmented 16% and 91% at 21 and 28 days respectively when PCBs were added to the N-limited liquid media. Mycoremediation of PCBs with P. sajor-caju LBM 105 cultivated in N-limited liquid medium is an effective low-cost (0.69 $ g-1d-1) alternative technique for PCBs treatment.Fil: Sadañoski, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Benitez, Silvana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaFil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; ArgentinaElsevier2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107329Sadañoski, Marcela Alejandra; Benitez, Silvana Florencia; Fonseca, Maria Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; et al.; Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment; Elsevier; Journal of Environmental Chemical Engineering; 7; 6; 12-2019; 1-282213-3437CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S2213343719305767info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2019.103453info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:38Zoai:ri.conicet.gov.ar:11336/107329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:38.382CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
title Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
spellingShingle Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
Sadañoski, Marcela Alejandra
POLYCHLORINATE BIPHENYLS
MYCOREMEDIATION
LACCASE
MORPHOLOGY
TOLERANCE
title_short Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
title_full Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
title_fullStr Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
title_full_unstemmed Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
title_sort Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment
dc.creator.none.fl_str_mv Sadañoski, Marcela Alejandra
Benitez, Silvana Florencia
Fonseca, Maria Isabel
Velázquez, Juan Ernesto
Zapata, Pedro Darío
Levin, Laura Noemí
Villalba, Laura Lidia
author Sadañoski, Marcela Alejandra
author_facet Sadañoski, Marcela Alejandra
Benitez, Silvana Florencia
Fonseca, Maria Isabel
Velázquez, Juan Ernesto
Zapata, Pedro Darío
Levin, Laura Noemí
Villalba, Laura Lidia
author_role author
author2 Benitez, Silvana Florencia
Fonseca, Maria Isabel
Velázquez, Juan Ernesto
Zapata, Pedro Darío
Levin, Laura Noemí
Villalba, Laura Lidia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv POLYCHLORINATE BIPHENYLS
MYCOREMEDIATION
LACCASE
MORPHOLOGY
TOLERANCE
topic POLYCHLORINATE BIPHENYLS
MYCOREMEDIATION
LACCASE
MORPHOLOGY
TOLERANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.5
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Polychlorinated biphenyls (PCBs) are exceptionally stable organic pollutants. An effective and economically feasible process is essential for their removal. Mycoremediation using white-rot fungal strains proved as a highly effective approach that has not been thoroughly investigated. The aim of this work was to evaluate PCBs remediation efficacy applying Pleurotus sajor-caju LBM 105. Axenic cultures of the fungus, either in complex or N-limited liquid media, were spiked with 217 mg L-1 of a technical mixture of Aroclor 1242, 1254 y 1260 in oil transformer. PCBs removal capacity, toxicity reduction, changes in fungal morphology and laccase expression caused by pollutant addition and the cost-effectiveness of the remediation treatment were assessed when growing the fungus under two different nutritional conditions. P. sajor-caju LBM 105 could remove 97.7% and 91.7% of the PCBs mixture and reduced the toxicity in complex and mineral media respectively after 35 days of incubation. The addition of PCBs to P. sajor-caju LBM 105 culture media resulted in an increase in hyphal diameter, changes in biomass, glucose consumption, proteins and laccase secretion. A 3-fold rise in laccase activity was detected in both media at 28 day. Laccase mRNA augmented 16% and 91% at 21 and 28 days respectively when PCBs were added to the N-limited liquid media. Mycoremediation of PCBs with P. sajor-caju LBM 105 cultivated in N-limited liquid medium is an effective low-cost (0.69 $ g-1d-1) alternative technique for PCBs treatment.
Fil: Sadañoski, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Benitez, Silvana Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Fonseca, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Velázquez, Juan Ernesto. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
Fil: Levin, Laura Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Bioquímica Clínica. Laboratorio de Biotecnología Molecular; Argentina
description Polychlorinated biphenyls (PCBs) are exceptionally stable organic pollutants. An effective and economically feasible process is essential for their removal. Mycoremediation using white-rot fungal strains proved as a highly effective approach that has not been thoroughly investigated. The aim of this work was to evaluate PCBs remediation efficacy applying Pleurotus sajor-caju LBM 105. Axenic cultures of the fungus, either in complex or N-limited liquid media, were spiked with 217 mg L-1 of a technical mixture of Aroclor 1242, 1254 y 1260 in oil transformer. PCBs removal capacity, toxicity reduction, changes in fungal morphology and laccase expression caused by pollutant addition and the cost-effectiveness of the remediation treatment were assessed when growing the fungus under two different nutritional conditions. P. sajor-caju LBM 105 could remove 97.7% and 91.7% of the PCBs mixture and reduced the toxicity in complex and mineral media respectively after 35 days of incubation. The addition of PCBs to P. sajor-caju LBM 105 culture media resulted in an increase in hyphal diameter, changes in biomass, glucose consumption, proteins and laccase secretion. A 3-fold rise in laccase activity was detected in both media at 28 day. Laccase mRNA augmented 16% and 91% at 21 and 28 days respectively when PCBs were added to the N-limited liquid media. Mycoremediation of PCBs with P. sajor-caju LBM 105 cultivated in N-limited liquid medium is an effective low-cost (0.69 $ g-1d-1) alternative technique for PCBs treatment.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107329
Sadañoski, Marcela Alejandra; Benitez, Silvana Florencia; Fonseca, Maria Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; et al.; Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment; Elsevier; Journal of Environmental Chemical Engineering; 7; 6; 12-2019; 1-28
2213-3437
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107329
identifier_str_mv Sadañoski, Marcela Alejandra; Benitez, Silvana Florencia; Fonseca, Maria Isabel; Velázquez, Juan Ernesto; Zapata, Pedro Darío; et al.; Mycoremediation of high concentrations of polychlorinated biphenyls with Pleurotus sajor-caju LBM 105 as an effective and cheap treatment; Elsevier; Journal of Environmental Chemical Engineering; 7; 6; 12-2019; 1-28
2213-3437
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S2213343719305767
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jece.2019.103453
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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