Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance

Autores
Roldán Gallardo, Franco Fabián; Dematteo, Karen E.; Rinas, Miguel Ángel; Argüelles, Carina Francisca
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Roldán Gallardo, Franco Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.
Fil: Dematteo, Karen E. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Dematteo, Karen E. Wildcare Institute at the Saint Louis Zoo; Estados Unidos.
Fil: Dematteo, Karen E. Washington University in St. Louis. Department of Biology & Environmental Studies; Estados Unidos.
Fil: Rinas, Miguel Ángel. Ministerio de Ecología y Recursos. Naturales Renovables (Misiones); Argentina.
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Argüelles, Carina Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.
Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplifcation of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplifcation profle in blood samples were subsequently applied to scats and saliva samples, to evaluate their efciency. Of the 13 microsatellite pairs that amplifed in blood, one-third (32%) were successfully amplifed in saliva and scat samples. This initial work demonstrates successful cross-amplif- cation in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
Materia
Cuniculus paca
Heterologous amplifcation
Microsatellite primers
Saliva Scat
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Repositorio
Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
Institución
Universidad Nacional de Misiones
OAI Identificador
oai:rid.unam.edu.ar:20.500.12219/5078
Acceder
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spelling Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importanceRoldán Gallardo, Franco FabiánDematteo, Karen E.Rinas, Miguel ÁngelArgüelles, Carina FranciscaCuniculus pacaHeterologous amplifcationMicrosatellite primersSaliva ScatFil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.Fil: Roldán Gallardo, Franco Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.Fil: Dematteo, Karen E. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.Fil: Dematteo, Karen E. Wildcare Institute at the Saint Louis Zoo; Estados Unidos.Fil: Dematteo, Karen E. Washington University in St. Louis. Department of Biology & Environmental Studies; Estados Unidos.Fil: Rinas, Miguel Ángel. Ministerio de Ecología y Recursos. Naturales Renovables (Misiones); Argentina.Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.Fil: Argüelles, Carina Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplifcation of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplifcation profle in blood samples were subsequently applied to scats and saliva samples, to evaluate their efciency. Of the 13 microsatellite pairs that amplifed in blood, one-third (32%) were successfully amplifed in saliva and scat samples. This initial work demonstrates successful cross-amplif- cation in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.BioMed Central2020-07-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf1012 KBhttps://hdl.handle.net/20.500.12219/5078enginfo:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/140665info:eu-repo/semantics/altIdentifier/urn/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05312-xinfo:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13104-020-05312-xinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)instname:Universidad Nacional de Misiones2025-09-29T15:02:15Zoai:rid.unam.edu.ar:20.500.12219/5078instacron:UNAMInstitucionalhttps://rid.unam.edu.ar/Universidad públicahttps://www.unam.edu.ar/https://rid.unam.edu.ar/oai/rsnrdArgentinaopendoar:2025-09-29 15:02:15.836Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misionesfalse
dc.title.none.fl_str_mv Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
title Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
spellingShingle Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
Roldán Gallardo, Franco Fabián
Cuniculus paca
Heterologous amplifcation
Microsatellite primers
Saliva Scat
title_short Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
title_full Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
title_fullStr Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
title_full_unstemmed Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
title_sort Heterologous microsatellite primers are informative for paca (cuniculus paca), a large rodent with economic and ecological importance
dc.creator.none.fl_str_mv Roldán Gallardo, Franco Fabián
Dematteo, Karen E.
Rinas, Miguel Ángel
Argüelles, Carina Francisca
author Roldán Gallardo, Franco Fabián
author_facet Roldán Gallardo, Franco Fabián
Dematteo, Karen E.
Rinas, Miguel Ángel
Argüelles, Carina Francisca
author_role author
author2 Dematteo, Karen E.
Rinas, Miguel Ángel
Argüelles, Carina Francisca
author2_role author
author
author
dc.subject.none.fl_str_mv Cuniculus paca
Heterologous amplifcation
Microsatellite primers
Saliva Scat
topic Cuniculus paca
Heterologous amplifcation
Microsatellite primers
Saliva Scat
dc.description.none.fl_txt_mv Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Roldán Gallardo, Franco Fabián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.
Fil: Dematteo, Karen E. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Dematteo, Karen E. Wildcare Institute at the Saint Louis Zoo; Estados Unidos.
Fil: Dematteo, Karen E. Washington University in St. Louis. Department of Biology & Environmental Studies; Estados Unidos.
Fil: Rinas, Miguel Ángel. Ministerio de Ecología y Recursos. Naturales Renovables (Misiones); Argentina.
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
Fil: Argüelles, Carina Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Subtropical; Argentina.
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina.
Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplifcation of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplifcation profle in blood samples were subsequently applied to scats and saliva samples, to evaluate their efciency. Of the 13 microsatellite pairs that amplifed in blood, one-third (32%) were successfully amplifed in saliva and scat samples. This initial work demonstrates successful cross-amplif- cation in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
description Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Genética; Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-07-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12219/5078
url https://hdl.handle.net/20.500.12219/5078
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/140665
info:eu-repo/semantics/altIdentifier/urn/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05312-x
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/s13104-020-05312-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 4.0 Internacional
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eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 4.0 Internacional
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
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