Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance

Autores
Roldán Gallardo, Franco Fabián; Dematteo, Karen; Rinas, Miguel Angel; Argüelles, Carina Francisca
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Dematteo, Karen. Wildcare Institute at the Saint Louis Zoo; Estados Unidos. Washington University in St. Louis; Estados Unidos
Fil: Rinas, Miguel Angel. Gobierno de la Provincia de Misiones. Ministerio de Ecología y Recursos Naturales Renovables; Argentina
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Materia
CUNICULUS PACA
HETEROLOGOUS AMPLIFICATION
MICROSATELLITE PRIMERS
SALIVA
SCAT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/140665
Acceder
id CONICETDig_b7f62ca7054109836d9f09cd1a8763f3
oai_identifier_str oai:ri.conicet.gov.ar:11336/140665
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importanceRoldán Gallardo, Franco FabiánDematteo, KarenRinas, Miguel AngelArgüelles, Carina FranciscaCUNICULUS PACAHETEROLOGOUS AMPLIFICATIONMICROSATELLITE PRIMERSSALIVASCAThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaFil: Dematteo, Karen. Wildcare Institute at the Saint Louis Zoo; Estados Unidos. Washington University in St. Louis; Estados UnidosFil: Rinas, Miguel Angel. Gobierno de la Provincia de Misiones. Ministerio de Ecología y Recursos Naturales Renovables; ArgentinaFil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaBioMed Central2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/140665Roldán Gallardo, Franco Fabián; Dematteo, Karen; Rinas, Miguel Angel; Argüelles, Carina Francisca; Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance; BioMed Central; BMC Research Notes; 13; 1; 10-2020; 1-71756-0500CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05312-xinfo:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-020-05312-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:47:57Zoai:ri.conicet.gov.ar:11336/140665instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:47:57.581CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
spellingShingle Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
Roldán Gallardo, Franco Fabián
CUNICULUS PACA
HETEROLOGOUS AMPLIFICATION
MICROSATELLITE PRIMERS
SALIVA
SCAT
title_short Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_full Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_fullStr Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_full_unstemmed Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
title_sort Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance
dc.creator.none.fl_str_mv Roldán Gallardo, Franco Fabián
Dematteo, Karen
Rinas, Miguel Angel
Argüelles, Carina Francisca
author Roldán Gallardo, Franco Fabián
author_facet Roldán Gallardo, Franco Fabián
Dematteo, Karen
Rinas, Miguel Angel
Argüelles, Carina Francisca
author_role author
author2 Dematteo, Karen
Rinas, Miguel Angel
Argüelles, Carina Francisca
author2_role author
author
author
dc.subject.none.fl_str_mv CUNICULUS PACA
HETEROLOGOUS AMPLIFICATION
MICROSATELLITE PRIMERS
SALIVA
SCAT
topic CUNICULUS PACA
HETEROLOGOUS AMPLIFICATION
MICROSATELLITE PRIMERS
SALIVA
SCAT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
Fil: Roldán Gallardo, Franco Fabián. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Fil: Dematteo, Karen. Wildcare Institute at the Saint Louis Zoo; Estados Unidos. Washington University in St. Louis; Estados Unidos
Fil: Rinas, Miguel Angel. Gobierno de la Provincia de Misiones. Ministerio de Ecología y Recursos Naturales Renovables; Argentina
Fil: Argüelles, Carina Francisca. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
description Objective: This study was designed to facilitate genetic studies that would allow information on population structure and genetic diversity of natural or captive stocks of paca (Cuniculus paca), a species of ecological and socioeconomic importance, by testing cross-amplification of 20 heterologous microsatellite primer pairs developed for guinea pigs (Cavia porcellus) and capybara (Hydrochoerus hydrochaeris). Results: Those primers that showed the best amplification profile in blood samples were subsequently applied to scats and saliva samples, to evaluate their efficiency. Of the 13 microsatellite pairs that amplified in blood, one-third (32%) were successfully amplified in saliva and scat samples. This initial work demonstrates successful cross-amplification in paca providing a solid and promising foundation for future genetic studies with this species. The ability to quantify genetic diversity using noninvasive samples from free-ranging paca is essential to developing applied management strategies for this large neotropical rodent that is not only a prey favored by wide-ranging carnivores [e.g., jaguar (Panthera onca), puma (Puma concolor)], but is also a species targeted by illegal hunting and wildlife trade.
publishDate 2020
dc.date.none.fl_str_mv 2020-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/140665
Roldán Gallardo, Franco Fabián; Dematteo, Karen; Rinas, Miguel Angel; Argüelles, Carina Francisca; Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance; BioMed Central; BMC Research Notes; 13; 1; 10-2020; 1-7
1756-0500
CONICET Digital
CONICET
url http://hdl.handle.net/11336/140665
identifier_str_mv Roldán Gallardo, Franco Fabián; Dematteo, Karen; Rinas, Miguel Angel; Argüelles, Carina Francisca; Heterologous microsatellite primers are informative for paca (Cuniculus paca), a large rodent with economic and ecological importance; BioMed Central; BMC Research Notes; 13; 1; 10-2020; 1-7
1756-0500
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05312-x
info:eu-repo/semantics/altIdentifier/doi/10.1186/s13104-020-05312-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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