Decolorization of Kraft liquor effluents and biochemical characterization of laccases from Phlebia brevispora BAFC 633

• Autores: Fonseca, María Isabel; Fariña, Julia Ines; Sadañoski, Marcela Alejandra; D'Errico, Roger; Villalba, Laura Lidia; Zapata, Pedro Darío
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: Phlebia brevispora BAFC 633; Laccase isoenzymes; Biochemical characterization; Bioremediation
• Institución: Universidad Nacional de Misiones

Fil: Fonseca, María Isabel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico (Tucumán). Planta Piloto de Procesos Industriales Microbiológicos. Departamento de Biotecnología Fúngica; Argentina.
Fil: Fariña, Julia Ines. Universidad Nacional de Catamarca. Facultad de Ciencias Exactas y Naturales. Cátedra de Química Biológica; Argentina.
Fil: Sadañoski, Marcela Alejandra. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: D'Errico, Roger. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
Fil: Zapata, Pedro Darío. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología Misiones “Dra. María Ebe Reca”. Laboratorio de Biotecnología Molecular; Argentina.
In this work we evaluated the decolorization ability of fungus identified as Phlebia brevispora BAFC 633 on Kraft liquor effluents and we looked at the biochemical characterization of two novel laccases from the fungus. We conducted decolorization assays on diluted Kraft liquor with the enzyme supernatants. Ligninolytic activity was demonstrated by the 20% reduction of lignin peak absorbances between 215 and 280 nm after laccase supernatants treatment. From these supernatants, two novel laccases were isolated and characterized: a constitutively expressed 60-kDa (Lac A), and a CuSO4-induced 75 kDa (Lac B). Laccases were purified by anion-exchange and gel filltration chromatography and their biochemical properties were determined. The temperature and pH optima were 70–75 °C and 4.8 for Lac A, and 60–70 °C and 4.4 for Lac B; and both exhibited high stability at low pH and high temperature. Substrate specificity of the purified enzyme was tested and the highest oxidation was to 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) with a catalytic efficiency of 2.6 × 107 s−1 mol l−1 for Lac A and 9 × 108 s−1 mol l−1 for Lac B. Enzyme supernatants can be used to remediate lignin-rich effluent. The isoenzymes tolerance to low pH and high temperature are significant features to optimize laccase production toward potential biotechnological applications.