Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633

Autores
Fonseca, Maria Isabel; Ramos Hryb, Ana Belén; Fariña, Julia Ines; Sawojstanic Afanasiuk, Silvana Soledad; Villalba, Laura Lidia; Zapata, Pedro Dario
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nine aromatic compounds (caffeic acid, syringaldehyde, vanillic acid, guaiacol, vanillin, sinapic acid, syringol, syringic acid and ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested for their ability to increase laccase (Lac) activity in the ligninolytic basidiomycete Phlebia brevispora BAFC 633.The addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect on fungal growth; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture. P. brevispora BAFC 633 produced two isoenzymes, a constitutively expressed of 60 kDa and another of 75 kDa expressed upon induction by sinapic acid, MnSO4 or CuSO4. Laccase secretion capacity of Phlebia brevispora BAFC 633 can be increased 27 times higher than the control with the highest levels detected in the presence of 0.3 mM CuSO4 at day 14. The action is affected at pre-transcriptional level regulating at the onset of the process, however it does not rule out the effect at the post-transcriptional and post-translational levels, for which is necessary to deepen in the knowledge of all possible regulation points of gene expression.
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Ramos Hryb, Ana Belén. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Sawojstanic Afanasiuk, Silvana Soledad. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Laccase
White Rot Fungi
Phlebia Brevispora Bafc 633
Izoenzymes
Induction
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/28009
Acceder
id CONICETDig_114142e4620a63c8adcae7c73e17e0cc
oai_identifier_str oai:ri.conicet.gov.ar:11336/28009
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633Fonseca, Maria IsabelRamos Hryb, Ana BelénFariña, Julia InesSawojstanic Afanasiuk, Silvana SoledadVillalba, Laura LidiaZapata, Pedro DarioLaccaseWhite Rot FungiPhlebia Brevispora Bafc 633IzoenzymesInductionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Nine aromatic compounds (caffeic acid, syringaldehyde, vanillic acid, guaiacol, vanillin, sinapic acid, syringol, syringic acid and ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested for their ability to increase laccase (Lac) activity in the ligninolytic basidiomycete Phlebia brevispora BAFC 633.The addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect on fungal growth; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture. P. brevispora BAFC 633 produced two isoenzymes, a constitutively expressed of 60 kDa and another of 75 kDa expressed upon induction by sinapic acid, MnSO4 or CuSO4. Laccase secretion capacity of Phlebia brevispora BAFC 633 can be increased 27 times higher than the control with the highest levels detected in the presence of 0.3 mM CuSO4 at day 14. The action is affected at pre-transcriptional level regulating at the onset of the process, however it does not rule out the effect at the post-transcriptional and post-translational levels, for which is necessary to deepen in the knowledge of all possible regulation points of gene expression.Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Ramos Hryb, Ana Belén. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Sawojstanic Afanasiuk, Silvana Soledad. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2014-03-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/28009Fonseca, Maria Isabel; Ramos Hryb, Ana Belén; Fariña, Julia Ines; Sawojstanic Afanasiuk, Silvana Soledad; Villalba, Laura Lidia; et al.; Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633; Springer; World Journal of Microbiology; 30; 8; 29-3-2014; 2251-22620959-39931573-0972CONICET DigitalCONICETenginfo:eu-repo/semantics/reference/url/https://link.springer.com/article/10.1007/s11274-014-1646-8info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-014-1646-8info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11274-014-1646-8info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:53:34Zoai:ri.conicet.gov.ar:11336/28009instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:53:34.947CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
title Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
spellingShingle Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
Fonseca, Maria Isabel
Laccase
White Rot Fungi
Phlebia Brevispora Bafc 633
Izoenzymes
Induction
title_short Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
title_full Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
title_fullStr Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
title_full_unstemmed Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
title_sort Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633
dc.creator.none.fl_str_mv Fonseca, Maria Isabel
Ramos Hryb, Ana Belén
Fariña, Julia Ines
Sawojstanic Afanasiuk, Silvana Soledad
Villalba, Laura Lidia
Zapata, Pedro Dario
author Fonseca, Maria Isabel
author_facet Fonseca, Maria Isabel
Ramos Hryb, Ana Belén
Fariña, Julia Ines
Sawojstanic Afanasiuk, Silvana Soledad
Villalba, Laura Lidia
Zapata, Pedro Dario
author_role author
author2 Ramos Hryb, Ana Belén
Fariña, Julia Ines
Sawojstanic Afanasiuk, Silvana Soledad
Villalba, Laura Lidia
Zapata, Pedro Dario
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Laccase
White Rot Fungi
Phlebia Brevispora Bafc 633
Izoenzymes
Induction
topic Laccase
White Rot Fungi
Phlebia Brevispora Bafc 633
Izoenzymes
Induction
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Nine aromatic compounds (caffeic acid, syringaldehyde, vanillic acid, guaiacol, vanillin, sinapic acid, syringol, syringic acid and ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested for their ability to increase laccase (Lac) activity in the ligninolytic basidiomycete Phlebia brevispora BAFC 633.The addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect on fungal growth; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture. P. brevispora BAFC 633 produced two isoenzymes, a constitutively expressed of 60 kDa and another of 75 kDa expressed upon induction by sinapic acid, MnSO4 or CuSO4. Laccase secretion capacity of Phlebia brevispora BAFC 633 can be increased 27 times higher than the control with the highest levels detected in the presence of 0.3 mM CuSO4 at day 14. The action is affected at pre-transcriptional level regulating at the onset of the process, however it does not rule out the effect at the post-transcriptional and post-translational levels, for which is necessary to deepen in the knowledge of all possible regulation points of gene expression.
Fil: Fonseca, Maria Isabel. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Ramos Hryb, Ana Belén. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Sawojstanic Afanasiuk, Silvana Soledad. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Villalba, Laura Lidia. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina
Fil: Zapata, Pedro Dario. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Nine aromatic compounds (caffeic acid, syringaldehyde, vanillic acid, guaiacol, vanillin, sinapic acid, syringol, syringic acid and ferulic acid) and four metallic compounds (CuSO4, AgNO3, MnSO4, and CaCl2) were tested for their ability to increase laccase (Lac) activity in the ligninolytic basidiomycete Phlebia brevispora BAFC 633.The addition of syringaldehyde, syringol, guaiacol, sinapic acid, vanillin, ferulic acid and CuSO4 showed a positive effect on fungal growth; however, it decreased dramatically with the addition of AgNO3 and did not undergo changes in the presence of CaCl2 or MnSO4. Lac activity increased with the addition of all the compounds tested, depending on the concentration and the day of culture. P. brevispora BAFC 633 produced two isoenzymes, a constitutively expressed of 60 kDa and another of 75 kDa expressed upon induction by sinapic acid, MnSO4 or CuSO4. Laccase secretion capacity of Phlebia brevispora BAFC 633 can be increased 27 times higher than the control with the highest levels detected in the presence of 0.3 mM CuSO4 at day 14. The action is affected at pre-transcriptional level regulating at the onset of the process, however it does not rule out the effect at the post-transcriptional and post-translational levels, for which is necessary to deepen in the knowledge of all possible regulation points of gene expression.
publishDate 2014
dc.date.none.fl_str_mv 2014-03-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/28009
Fonseca, Maria Isabel; Ramos Hryb, Ana Belén; Fariña, Julia Ines; Sawojstanic Afanasiuk, Silvana Soledad; Villalba, Laura Lidia; et al.; Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633; Springer; World Journal of Microbiology; 30; 8; 29-3-2014; 2251-2262
0959-3993
1573-0972
CONICET Digital
CONICET
url http://hdl.handle.net/11336/28009
identifier_str_mv Fonseca, Maria Isabel; Ramos Hryb, Ana Belén; Fariña, Julia Ines; Sawojstanic Afanasiuk, Silvana Soledad; Villalba, Laura Lidia; et al.; Effect of chemical and metallic compounds on biomass, mRNA levels and laccase activity of Phlebia brevispora BAFC 633; Springer; World Journal of Microbiology; 30; 8; 29-3-2014; 2251-2262
0959-3993
1573-0972
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/reference/url/https://link.springer.com/article/10.1007/s11274-014-1646-8
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11274-014-1646-8
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11274-014-1646-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
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dc.format.none.fl_str_mv application/pdf
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application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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