Sex chromosome complement determines sex differences in aromatase expression and influences aromatase regulation by gonadal hormones in developing neurons

• Autores: Cambiasso, María Julia
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: documento de conferencia
• Materia: Aromatase; Sex chromosomes; Sexual differentiation
• Institución: Universidad Nacional de Córdoba

Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
The current model of sexual differentiation involves genetic factors encoded by sex chromosomes acting in parallel with gonadal factors. We evaluated the role of sex chromosome complement on the generation of sex differences in the expression of aromatase in specific brain regions in mouse embryos at E16. For this study we used the "four core genotypes" mouse model, in which the effect of gonadal sex and sex chromosome complement is dissociated. The brain of XY embryos showed higher levels of aromatase expression in the stria terminalis and the anterior amygdaloid area than the brain of XX embryos, with independence of their gonadal sex. Furthermore, estradiol or dihydrotestosterone increased the expression of aromatase in cultures of amygdaline neurons derived from XX embryos, but not in those derived from XY embryos, with independence of their gonadal sex. Sex chromosome complement was not involved in the regulation of the expression of other steroidogenic molecules, such as StAR, P450scc or 5α-reductase and was not involved in the generation of sex differences in the expression of estrogen receptor-α and androgen receptor in aromatase-positive brain regions, which depended on gonadal sex. In conclusion, sex chromosome complement determine the expression of aromatase and the response of aromatase expressing neurons to gonadal hormones and both sex chromosome factors and gonadal factors regulate the expression of molecules involved in the generation of brain sex differences, such as aromatase, estrogen receptor-α and androgen receptor.
Fil: Cambiasso, María Julia. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Biología Celular B; Argentina.
Fil: Cambiasso, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Bioquímica y Biología Molecular (ídem 3.1.10)