UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications

Autores
Minchiotti, Mariana Cecilia
Año de publicación
2025
Idioma
inglés
Tipo de recurso
parte de libro
Estado
versión publicada
Descripción
Minchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.
The UV-Vis spectroscopy is very useful to determine molecules or aggregates with chromophore groups. These groups are regions that have double or triple bonds that can absorb energy to promote the electrons of these molecular orbitals to excited states. Then, these electrons will return to their basal level releasing the absorbed energy. These energetic transitions are all different depending on the double or triple bonds and the environment (if they present auxochromes or if there is a conjugated system). This allows to identify different molecular aggregates, complexes or molecules. In this way, it is possible to follow enzymatic reactions in real time or concentration of chelates formed.
info:eu-repo/semantics/publishedVersion
Minchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.
Fuente
DOI: 10.5772/intechopen.1001672
Materia
Biotecnología
Espectrometría
Espectroscopia ultravioleta
Moléculas
Absorción
Energía
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/556536
Acceder
id RDUUNC_bfd03d9f484e468b35abcae00fef72cf
oai_identifier_str oai:rdu.unc.edu.ar:11086/556536
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applicationsMinchiotti, Mariana CeciliaBiotecnologíaEspectrometríaEspectroscopia ultravioletaMoléculasAbsorciónEnergíaMinchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.The UV-Vis spectroscopy is very useful to determine molecules or aggregates with chromophore groups. These groups are regions that have double or triple bonds that can absorb energy to promote the electrons of these molecular orbitals to excited states. Then, these electrons will return to their basal level releasing the absorbed energy. These energetic transitions are all different depending on the double or triple bonds and the environment (if they present auxochromes or if there is a conjugated system). This allows to identify different molecular aggregates, complexes or molecules. In this way, it is possible to follow enzymatic reactions in real time or concentration of chelates formed.info:eu-repo/semantics/publishedVersionMinchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.2025info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookParthttp://purl.org/coar/resource_type/c_3248info:ar-repo/semantics/parteDeLibroapplication/pdfMinchiotti, M. C. (2025). UV-visible spectrophotometry: introduction, quantification, equipment and biotechnological applications. En Nirmal Mazumder & Guan-Yu Zhuo, editores. Recent advances in infrared spectroscopy and its applications in biotechnologyIntech (pág. 119-136). Intechopen. DOI: 10.5772/intechopen.10071479780850149210http://hdl.handle.net/11086/556536DOI: 10.5772/intechopen.1007147DOI: 10.5772/intechopen.1001672reponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNCenginfo:eu-repo/semantics/openAccess2025-09-29T13:43:20Zoai:rdu.unc.edu.ar:11086/556536Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:43:20.214Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
title UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
spellingShingle UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
Minchiotti, Mariana Cecilia
Biotecnología
Espectrometría
Espectroscopia ultravioleta
Moléculas
Absorción
Energía
title_short UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
title_full UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
title_fullStr UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
title_full_unstemmed UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
title_sort UV-visible spectrophotometry : introduction, quantification, equipment and biotechnological applications
dc.creator.none.fl_str_mv Minchiotti, Mariana Cecilia
author Minchiotti, Mariana Cecilia
author_facet Minchiotti, Mariana Cecilia
author_role author
dc.subject.none.fl_str_mv Biotecnología
Espectrometría
Espectroscopia ultravioleta
Moléculas
Absorción
Energía
topic Biotecnología
Espectrometría
Espectroscopia ultravioleta
Moléculas
Absorción
Energía
dc.description.none.fl_txt_mv Minchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.
The UV-Vis spectroscopy is very useful to determine molecules or aggregates with chromophore groups. These groups are regions that have double or triple bonds that can absorb energy to promote the electrons of these molecular orbitals to excited states. Then, these electrons will return to their basal level releasing the absorbed energy. These energetic transitions are all different depending on the double or triple bonds and the environment (if they present auxochromes or if there is a conjugated system). This allows to identify different molecular aggregates, complexes or molecules. In this way, it is possible to follow enzymatic reactions in real time or concentration of chelates formed.
info:eu-repo/semantics/publishedVersion
Minchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.
description Minchiotti, Mariana Cecilia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/bookPart
http://purl.org/coar/resource_type/c_3248
info:ar-repo/semantics/parteDeLibro
status_str publishedVersion
format bookPart
dc.identifier.none.fl_str_mv Minchiotti, M. C. (2025). UV-visible spectrophotometry: introduction, quantification, equipment and biotechnological applications. En Nirmal Mazumder & Guan-Yu Zhuo, editores. Recent advances in infrared spectroscopy and its applications in biotechnologyIntech (pág. 119-136). Intechopen. DOI: 10.5772/intechopen.1007147
9780850149210
http://hdl.handle.net/11086/556536
DOI: 10.5772/intechopen.1007147
identifier_str_mv Minchiotti, M. C. (2025). UV-visible spectrophotometry: introduction, quantification, equipment and biotechnological applications. En Nirmal Mazumder & Guan-Yu Zhuo, editores. Recent advances in infrared spectroscopy and its applications in biotechnologyIntech (pág. 119-136). Intechopen. DOI: 10.5772/intechopen.1007147
9780850149210
DOI: 10.5772/intechopen.1007147
url http://hdl.handle.net/11086/556536
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv DOI: 10.5772/intechopen.1001672
reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 13.069144

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