Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells
- Autores
- Garay, María Isabel; Brunotto, Mabel; Rabaglino, Belén; Barotto, Nelso Neri; Quiroga, Patricia Liliana; Repossi Marquez, Pablo Gastón; Fernández-Zapico, Martín Ernesto; Pasqualini, María Eugenia
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.
Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.
Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos.
Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina.
Pancreatic carcinogenesis is characterized by the activation of inflammatory signaling pathways. Numerous studies have demonstrated the role of essential fatty acids (efas) in pancreatic cancer initiation and progression, but the underlying molecular mechanisms have not been completely elucidated. Here, we studied the effects of two omega-3 efas, the eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) and the omega-6 fatty acid, the arachidonic acid (aa) on a human pancreatic cell line (panc-1). We determined the release of interleukin-6 (il-6) as inflammatory marker in supernatants from panc-1 cultures using elisa, as well as their gene expression by qrt-pcr. Cell viability (rezasurin) and reactive oxygen species (ros) production (dcfh-da assay kit) by fluorimetry. Eicosanoids generation and lipid cell profile by high pressure liquid and gas chromatography (hplc and gc).We observed a significant reduction in cell viability in dha and epa compared to aa and control (ethanol) treatments (p<0.05). Moreover, we demonstrated an increased apoptotic levels in dha and epa treated cells in comparison with the control and aa treatment, probably mediated by ros production (p <0.05). A diminution of il-6 gene expression (p< 0.01) and liberation (p< 0.02) resulted after dha and epa treated cells. The release of proinflammatory eicosanoids: 13- hode and 5(s)-hete decreased on epa and dha treated cells compared with aa and control (p<0.05).Therefore, we demonstrated that omega-3 efas may reduce the growth of panc-1 by several mechanisms that include significant increase of ros production, diminution of lipid peroxides and down regulation of gene expression and secretion of inflammatory cytokines such as il-6
Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.
Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.
Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.
Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos.
Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina.
Bioquímica y Biología Molecular (ídem 1.6.3) - Materia
-
Pancreatic cancer
Essential fatty acids
Eicosanoids - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Universidad Nacional de Córdoba
- OAI Identificador
- oai:rdu.unc.edu.ar:11086/561060
Ver los metadatos del registro completo
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Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cellsGaray, María IsabelBrunotto, MabelRabaglino, BelénBarotto, Nelso NeriQuiroga, Patricia LilianaRepossi Marquez, Pablo GastónFernández-Zapico, Martín ErnestoPasqualini, María EugeniaPancreatic cancerEssential fatty acidsEicosanoidsFil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina.Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos.Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina.Pancreatic carcinogenesis is characterized by the activation of inflammatory signaling pathways. Numerous studies have demonstrated the role of essential fatty acids (efas) in pancreatic cancer initiation and progression, but the underlying molecular mechanisms have not been completely elucidated. Here, we studied the effects of two omega-3 efas, the eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) and the omega-6 fatty acid, the arachidonic acid (aa) on a human pancreatic cell line (panc-1). We determined the release of interleukin-6 (il-6) as inflammatory marker in supernatants from panc-1 cultures using elisa, as well as their gene expression by qrt-pcr. Cell viability (rezasurin) and reactive oxygen species (ros) production (dcfh-da assay kit) by fluorimetry. Eicosanoids generation and lipid cell profile by high pressure liquid and gas chromatography (hplc and gc).We observed a significant reduction in cell viability in dha and epa compared to aa and control (ethanol) treatments (p<0.05). Moreover, we demonstrated an increased apoptotic levels in dha and epa treated cells in comparison with the control and aa treatment, probably mediated by ros production (p <0.05). A diminution of il-6 gene expression (p< 0.01) and liberation (p< 0.02) resulted after dha and epa treated cells. The release of proinflammatory eicosanoids: 13- hode and 5(s)-hete decreased on epa and dha treated cells compared with aa and control (p<0.05).Therefore, we demonstrated that omega-3 efas may reduce the growth of panc-1 by several mechanisms that include significant increase of ros production, diminution of lipid peroxides and down regulation of gene expression and secretion of inflammatory cytokines such as il-6Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina.Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina.Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina.Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos.Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina.Bioquímica y Biología Molecular (ídem 1.6.3)2017info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdf1669-9106http://hdl.handle.net/11086/561060enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2026-04-23T10:33:22Zoai:rdu.unc.edu.ar:11086/561060Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722026-04-23 10:33:23.075Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse |
| dc.title.none.fl_str_mv |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| title |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| spellingShingle |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells Garay, María Isabel Pancreatic cancer Essential fatty acids Eicosanoids |
| title_short |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| title_full |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| title_fullStr |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| title_full_unstemmed |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| title_sort |
Omega-3 fatty acids mediate immune regulation anticancer mechanisms by il-6, 5 (s)- hete and ros generation in pancreatic human cells |
| dc.creator.none.fl_str_mv |
Garay, María Isabel Brunotto, Mabel Rabaglino, Belén Barotto, Nelso Neri Quiroga, Patricia Liliana Repossi Marquez, Pablo Gastón Fernández-Zapico, Martín Ernesto Pasqualini, María Eugenia |
| author |
Garay, María Isabel |
| author_facet |
Garay, María Isabel Brunotto, Mabel Rabaglino, Belén Barotto, Nelso Neri Quiroga, Patricia Liliana Repossi Marquez, Pablo Gastón Fernández-Zapico, Martín Ernesto Pasqualini, María Eugenia |
| author_role |
author |
| author2 |
Brunotto, Mabel Rabaglino, Belén Barotto, Nelso Neri Quiroga, Patricia Liliana Repossi Marquez, Pablo Gastón Fernández-Zapico, Martín Ernesto Pasqualini, María Eugenia |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
Pancreatic cancer Essential fatty acids Eicosanoids |
| topic |
Pancreatic cancer Essential fatty acids Eicosanoids |
| dc.description.none.fl_txt_mv |
Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina. Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos. Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina. Pancreatic carcinogenesis is characterized by the activation of inflammatory signaling pathways. Numerous studies have demonstrated the role of essential fatty acids (efas) in pancreatic cancer initiation and progression, but the underlying molecular mechanisms have not been completely elucidated. Here, we studied the effects of two omega-3 efas, the eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) and the omega-6 fatty acid, the arachidonic acid (aa) on a human pancreatic cell line (panc-1). We determined the release of interleukin-6 (il-6) as inflammatory marker in supernatants from panc-1 cultures using elisa, as well as their gene expression by qrt-pcr. Cell viability (rezasurin) and reactive oxygen species (ros) production (dcfh-da assay kit) by fluorimetry. Eicosanoids generation and lipid cell profile by high pressure liquid and gas chromatography (hplc and gc).We observed a significant reduction in cell viability in dha and epa compared to aa and control (ethanol) treatments (p<0.05). Moreover, we demonstrated an increased apoptotic levels in dha and epa treated cells in comparison with the control and aa treatment, probably mediated by ros production (p <0.05). A diminution of il-6 gene expression (p< 0.01) and liberation (p< 0.02) resulted after dha and epa treated cells. The release of proinflammatory eicosanoids: 13- hode and 5(s)-hete decreased on epa and dha treated cells compared with aa and control (p<0.05).Therefore, we demonstrated that omega-3 efas may reduce the growth of panc-1 by several mechanisms that include significant increase of ros production, diminution of lipid peroxides and down regulation of gene expression and secretion of inflammatory cytokines such as il-6 Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina. Fil: Rabaglino, Belén. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Fil: Barotto, Nelso Neri. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Quiroga, Patricia Liliana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Biología Celular, Histología y Embriología; Argentina. Fil: Repossi Marquez, Pablo Gastón. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina. Fil: Fernández-Zapico, Martín Ernesto, Mayo Clinic; Estados Unidos. Fil: Pasqualini, María Eugenia. Universidad Nacional de Córdoba. Cátedra de Biología Celular, Histología y Embriología; Argentina. Bioquímica y Biología Molecular (ídem 1.6.3) |
| description |
Fil: Garay, María Isabel. Universidad Nacional de Córdoba. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias de la Salud; Argentina. |
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2017 |
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2017 |
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