Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents

Autores
Moura Ibiapino, Amalia; Urdampilleta, Juan; García, Miguel A.; Pedrosa-Harand, Andrea; Stefanović, Saša; Costea, Mihai
Año de publicación
2024
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.
Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.
Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.
Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.
Abstract: Genus Cuscuta L. (Convolvulaceae) exhibits cases of hybridization and allopolyploidy. Section Denticulatae, subg. Grammica, includes four species: the allopolyploids, C. veatchii and C. psorothamnensis (2n = 60), which originated from two independent reticulation events between the diploids, C. denticulata and C. nevadensis (2n = 30). The allopolyploids are morphologically similar, but are differing in their geographical distribution and host specificity. While cytogenetic data have been reported for C. veatchii, this study aims to provide a comparative analysis with C. psorothamnensis. To characterize the chromosomal complement of C. psorothamnensis and compare it with C. veatchii, we used CMA/DAPI banding, FISH, and GISH. The karyotypes of both species displayed similarity in chromosome number, size, and symmetry, and interphase nucleus organization. Both species exhibited a pair of 5S and 35S rDNA sites adjacent on the same chromosome. The number of 5S rDNA sites in C. psorothamnensis is variable, with some individuals displaying four, five, and six sites. Our results show: 1) the chromosomal pair carrying adjacent 5S and 35S rDNA in C. denticulata is retained in the polyploids; 2) the loss of C. nevadensis rDNA sites occurred in both tetraploids; 3) C. psorothamnensis and C. veatchii are allopolyploids part of a species complex, originated from successive independent hybridization events between C. denticulata and C. nevadensis; and 4) C. psorothamnensis is probably more recent in origin than C. veatchii based on the degree of diploidization. This cytogenetic comparison allows us to understand the processes involved in the emergence of new polyploid species by hybridization.
info:eu-repo/semantics/publishedVersion
Fil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.
Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.
Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.
Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.
Materia
Cuscuta
Allopolyploidy
GISH
Parasitic plants
Reticulate evolution
Speciation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/553346
Acceder
id RDUUNC_4c18a8c517fb8bced93744ad1cb8e78f
oai_identifier_str oai:rdu.unc.edu.ar:11086/553346
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parentsMoura Ibiapino, AmaliaUrdampilleta, JuanGarcía, Miguel A.Pedrosa-Harand, AndreaStefanović, SašaCostea, MihaiCuscutaAllopolyploidyGISHParasitic plantsReticulate evolutionSpeciationFil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.Abstract: Genus Cuscuta L. (Convolvulaceae) exhibits cases of hybridization and allopolyploidy. Section Denticulatae, subg. Grammica, includes four species: the allopolyploids, C. veatchii and C. psorothamnensis (2n = 60), which originated from two independent reticulation events between the diploids, C. denticulata and C. nevadensis (2n = 30). The allopolyploids are morphologically similar, but are differing in their geographical distribution and host specificity. While cytogenetic data have been reported for C. veatchii, this study aims to provide a comparative analysis with C. psorothamnensis. To characterize the chromosomal complement of C. psorothamnensis and compare it with C. veatchii, we used CMA/DAPI banding, FISH, and GISH. The karyotypes of both species displayed similarity in chromosome number, size, and symmetry, and interphase nucleus organization. Both species exhibited a pair of 5S and 35S rDNA sites adjacent on the same chromosome. The number of 5S rDNA sites in C. psorothamnensis is variable, with some individuals displaying four, five, and six sites. Our results show: 1) the chromosomal pair carrying adjacent 5S and 35S rDNA in C. denticulata is retained in the polyploids; 2) the loss of C. nevadensis rDNA sites occurred in both tetraploids; 3) C. psorothamnensis and C. veatchii are allopolyploids part of a species complex, originated from successive independent hybridization events between C. denticulata and C. nevadensis; and 4) C. psorothamnensis is probably more recent in origin than C. veatchii based on the degree of diploidization. This cytogenetic comparison allows us to understand the processes involved in the emergence of new polyploid species by hybridization.info:eu-repo/semantics/publishedVersionFil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.https://orcid.org/0000-0002-2613-5259http://orcid.org/0000-0001-9922-2866http://orcid.org/0000-0001-5213-4770http://orcid.org/0000-0003-3049-17632024-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfMoura, A. I., Urdampilleta, J., Garcia, M. A., Pedrosa-Harand, A., Stefanovic, S., & Costea, M. (2024). Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents. bioRxiv, 2024-04.http://hdl.handle.net/11086/553346https://doi.org/10.1101/2024.04.15.589596spainfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-04T12:32:15Zoai:rdu.unc.edu.ar:11086/553346Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-04 12:32:15.735Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
title Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
spellingShingle Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
Moura Ibiapino, Amalia
Cuscuta
Allopolyploidy
GISH
Parasitic plants
Reticulate evolution
Speciation
title_short Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
title_full Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
title_fullStr Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
title_full_unstemmed Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
title_sort Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents
dc.creator.none.fl_str_mv Moura Ibiapino, Amalia
Urdampilleta, Juan
García, Miguel A.
Pedrosa-Harand, Andrea
Stefanović, Saša
Costea, Mihai
author Moura Ibiapino, Amalia
author_facet Moura Ibiapino, Amalia
Urdampilleta, Juan
García, Miguel A.
Pedrosa-Harand, Andrea
Stefanović, Saša
Costea, Mihai
author_role author
author2 Urdampilleta, Juan
García, Miguel A.
Pedrosa-Harand, Andrea
Stefanović, Saša
Costea, Mihai
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv https://orcid.org/0000-0002-2613-5259
http://orcid.org/0000-0001-9922-2866
http://orcid.org/0000-0001-5213-4770
http://orcid.org/0000-0003-3049-1763
dc.subject.none.fl_str_mv Cuscuta
Allopolyploidy
GISH
Parasitic plants
Reticulate evolution
Speciation
topic Cuscuta
Allopolyploidy
GISH
Parasitic plants
Reticulate evolution
Speciation
dc.description.none.fl_txt_mv Fil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.
Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.
Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.
Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.
Abstract: Genus Cuscuta L. (Convolvulaceae) exhibits cases of hybridization and allopolyploidy. Section Denticulatae, subg. Grammica, includes four species: the allopolyploids, C. veatchii and C. psorothamnensis (2n = 60), which originated from two independent reticulation events between the diploids, C. denticulata and C. nevadensis (2n = 30). The allopolyploids are morphologically similar, but are differing in their geographical distribution and host specificity. While cytogenetic data have been reported for C. veatchii, this study aims to provide a comparative analysis with C. psorothamnensis. To characterize the chromosomal complement of C. psorothamnensis and compare it with C. veatchii, we used CMA/DAPI banding, FISH, and GISH. The karyotypes of both species displayed similarity in chromosome number, size, and symmetry, and interphase nucleus organization. Both species exhibited a pair of 5S and 35S rDNA sites adjacent on the same chromosome. The number of 5S rDNA sites in C. psorothamnensis is variable, with some individuals displaying four, five, and six sites. Our results show: 1) the chromosomal pair carrying adjacent 5S and 35S rDNA in C. denticulata is retained in the polyploids; 2) the loss of C. nevadensis rDNA sites occurred in both tetraploids; 3) C. psorothamnensis and C. veatchii are allopolyploids part of a species complex, originated from successive independent hybridization events between C. denticulata and C. nevadensis; and 4) C. psorothamnensis is probably more recent in origin than C. veatchii based on the degree of diploidization. This cytogenetic comparison allows us to understand the processes involved in the emergence of new polyploid species by hybridization.
info:eu-repo/semantics/publishedVersion
Fil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Ibiapino, Amalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Urdampilleta, Juan. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
Fil: Urdampilleta, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: García, Miguel A. Real Jardín Botánico-CSIC, Madrid; Spain.
Fil: Pedrosa-Harand, Andrea. Universidade Federal de Pernambuco, Recife; Brazil.
Fil: Stefanović, Saša. University of Toronto Mississauga. Department of Biology; Canadá.
Fil: Costea, Mihai. University of Wilfrid Laurier. Department of Biology, Waterloo; Canadá.
description Fil: Moura Ibiapino, Amalia. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentina.
publishDate 2024
dc.date.none.fl_str_mv 2024-04
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str publishedVersion
format article
dc.identifier.none.fl_str_mv Moura, A. I., Urdampilleta, J., Garcia, M. A., Pedrosa-Harand, A., Stefanovic, S., & Costea, M. (2024). Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents. bioRxiv, 2024-04.
http://hdl.handle.net/11086/553346
https://doi.org/10.1101/2024.04.15.589596
identifier_str_mv Moura, A. I., Urdampilleta, J., Garcia, M. A., Pedrosa-Harand, A., Stefanovic, S., & Costea, M. (2024). Cytogenetic comparison of Cuscuta psorothamnensis and C. veatchii (Convolvulaceae), two species originated from recurrent hybridization between the same diploid parents. bioRxiv, 2024-04.
url http://hdl.handle.net/11086/553346
https://doi.org/10.1101/2024.04.15.589596
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 13.13397

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