Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain

Autores
Moliné, Martín; Libkind, Diego; van Broock, María Rosa
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites.
Fil: Moliné, Martín. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.
Fil: Libkind, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.
Fuente
World Journal of Microbiology and Biotechnology.
Materia
Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Digital Institucional (UNCo)
Institución
Universidad Nacional del Comahue
OAI Identificador
oai:rdi.uncoma.edu.ar:uncomaid/18810

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network_acronym_str RDIUNCO
repository_id_str 7108
network_name_str Repositorio Digital Institucional (UNCo)
spelling Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strainMoliné, MartínLibkind, Diegovan Broock, María RosaPhaa rhodozymaMycosporine-glutaminol-glucosideCarotenoidsAstaxanthinCiencias de la Tierra y Medio AmbientePhaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites.Fil: Moliné, Martín. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.Fil: Libkind, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.Springer2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfpp.1-27application/pdfhttps://rdi.uncoma.edu.ar/handle/uncomaid/18810World Journal of Microbiology and Biotechnology.reponame:Repositorio Digital Institucional (UNCo)instname:Universidad Nacional del Comahueenghttps://doi.org/10.21203/rs.3.rs-3495924/v1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-09-29T14:28:42Zoai:rdi.uncoma.edu.ar:uncomaid/18810instacron:UNCoInstitucionalhttp://rdi.uncoma.edu.ar/Universidad públicaNo correspondehttp://rdi.uncoma.edu.ar/oaimirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:71082025-09-29 14:28:43.041Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahuefalse
dc.title.none.fl_str_mv Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
spellingShingle Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
Moliné, Martín
Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
title_short Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_full Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_fullStr Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_full_unstemmed Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
title_sort Two at once: simultaneous increased production of astaxanthin and mycosporines in a single Batch culture using a Phaffia rhodozyma mutant strain
dc.creator.none.fl_str_mv Moliné, Martín
Libkind, Diego
van Broock, María Rosa
author Moliné, Martín
author_facet Moliné, Martín
Libkind, Diego
van Broock, María Rosa
author_role author
author2 Libkind, Diego
van Broock, María Rosa
author2_role author
author
dc.subject.none.fl_str_mv Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
topic Phaa rhodozyma
Mycosporine-glutaminol-glucoside
Carotenoids
Astaxanthin
Ciencias de la Tierra y Medio Ambiente
dc.description.none.fl_txt_mv Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites.
Fil: Moliné, Martín. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.
Fil: Libkind, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina.
description Phaffia rhodozyma is a basidiomycetous yeast characterized by its production of the carotenoid pigment astaxanthin, which holds high commercial value for its significance in aquaculture, cosmetics and as nutraceutics, and the UV-B-absorbing compound mycosporine-glutaminol-glucoside (MGG), which is of great biotechnological relevance for its incorporation into natural sunscreens. However, the industrial exploitation has been limited to the production of astaxanthin in small quantities. On the other hand, the accumulation of MGG in P. rhodozyma was recently reported and could add value to the simultaneous production of both metabolites. In this work, we obtain a mutant strain that overproduces both compounds, furthermore we determined how the accumulation of each is affected by the carbon-to-nitrogen ratio and six biotic and abiotic factors. The mutant obtained produces 159% more astaxanthin and 220% more MGG than the parental strain. Furthermore, we establish that the carotenoids accumulate during the exponential growth phase while MGG accumulates during the stationary phase. The carbon-to-nitrogen ratio affects each metabolite differently, high ratios favoring carotenoid accumulation while low ratios favoring MGG accumulation. Finally, the accumulation of both metabolites is stimulated only by photosynthetically active radiation and low concentrations of hydrogen peroxide. The mutant strain obtained is the first hyper-productive mutant capable of accumulating high concentrations of MGG and astaxanthin described to date. The characterization of how both compounds accumulate during growth and the factors that stimulate their accumulation, are the first steps toward the future commercial exploitation of strains for the simultaneous production of two biotechnologically important metabolites.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://rdi.uncoma.edu.ar/handle/uncomaid/18810
url https://rdi.uncoma.edu.ar/handle/uncomaid/18810
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.21203/rs.3.rs-3495924/v1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
pp.1-27
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv World Journal of Microbiology and Biotechnology.
reponame:Repositorio Digital Institucional (UNCo)
instname:Universidad Nacional del Comahue
reponame_str Repositorio Digital Institucional (UNCo)
collection Repositorio Digital Institucional (UNCo)
instname_str Universidad Nacional del Comahue
repository.name.fl_str_mv Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahue
repository.mail.fl_str_mv mirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.ar
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