The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.

Autores
David Palma, Márcia; Libkind Frati, Diego; Brito, Patrícia H.; Silva, Margarida; Bellora, Nicolás; Coelho, Marco A.; Heitman, Joseph; Gonçalves, Paula; Sampaio, José Paulo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phaffia is an orange-colored basidiomycetous yeast genus of the order Cystofilobasidiales that contains a single species, P. rhodozyma. This species is the only fungus known to produce the economically relevant carotenoid astaxanthin. Although Phaffia was originally found in the Northern hemisphere, its diversity in the southern part of the globe has been shown to be much greater. Here we analyze the genomes of two Australasian lineages that are markedly distinct from P. rhodozyma. The two divergent lineages were investigated within a comprehensive phylogenomic study of representatives of the Cystofilobasidiales that supported the recognition of two novel Phaffia species, for which we propose the names of P. australis sp. nov. and P. tasmanica sp. nov. Comparative genomics and other analyses confirmed that the two new species have the typical Phaffia hallmark—the six genes necessary for the biosynthesis of astaxanthin could be retrieved from the draft genome sequences, and this carotenoid was detected in culture extracts. In addition, the organization of the mating-type (MAT) loci is similar to that of P. rhodozyma, with synteny throughout most regions. Moreover, cases of trans-specific polymorphism involving pheromone receptor genes and pheromone precursor proteins in the three Phaffia species, together with their shared homothallism, provide additional support for their classification in a single genus.
Fil: David Palma, Márcia. University of Duke; Estados Unidos. Universidade Nova de Lisboa; Portugal
Fil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Brito, Patrícia H.. Universidade Nova de Lisboa; Portugal
Fil: Silva, Margarida. Universidade Nova de Lisboa; Portugal
Fil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina
Fil: Coelho, Marco A.. Universidade Nova de Lisboa; Portugal
Fil: Heitman, Joseph. University of Duke; Estados Unidos
Fil: Gonçalves, Paula. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;
Fil: Sampaio, José Paulo. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;
Materia
ASTAXANTHIN
FUNGAL MAT GENES
PHAFFIA
PHYLOGENOMICS
YEAST TAXONOMY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/183651
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/183651
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.David Palma, MárciaLibkind Frati, DiegoBrito, Patrícia H.Silva, MargaridaBellora, NicolásCoelho, Marco A.Heitman, JosephGonçalves, PaulaSampaio, José PauloASTAXANTHINFUNGAL MAT GENESPHAFFIAPHYLOGENOMICSYEAST TAXONOMYhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phaffia is an orange-colored basidiomycetous yeast genus of the order Cystofilobasidiales that contains a single species, P. rhodozyma. This species is the only fungus known to produce the economically relevant carotenoid astaxanthin. Although Phaffia was originally found in the Northern hemisphere, its diversity in the southern part of the globe has been shown to be much greater. Here we analyze the genomes of two Australasian lineages that are markedly distinct from P. rhodozyma. The two divergent lineages were investigated within a comprehensive phylogenomic study of representatives of the Cystofilobasidiales that supported the recognition of two novel Phaffia species, for which we propose the names of P. australis sp. nov. and P. tasmanica sp. nov. Comparative genomics and other analyses confirmed that the two new species have the typical Phaffia hallmark—the six genes necessary for the biosynthesis of astaxanthin could be retrieved from the draft genome sequences, and this carotenoid was detected in culture extracts. In addition, the organization of the mating-type (MAT) loci is similar to that of P. rhodozyma, with synteny throughout most regions. Moreover, cases of trans-specific polymorphism involving pheromone receptor genes and pheromone precursor proteins in the three Phaffia species, together with their shared homothallism, provide additional support for their classification in a single genus.Fil: David Palma, Márcia. University of Duke; Estados Unidos. Universidade Nova de Lisboa; PortugalFil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; ArgentinaFil: Brito, Patrícia H.. Universidade Nova de Lisboa; PortugalFil: Silva, Margarida. Universidade Nova de Lisboa; PortugalFil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Coelho, Marco A.. Universidade Nova de Lisboa; PortugalFil: Heitman, Joseph. University of Duke; Estados UnidosFil: Gonçalves, Paula. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;Fil: Sampaio, José Paulo. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;Multidisciplinary Digital Publishing Institute2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/183651David Palma, Márcia; Libkind Frati, Diego; Brito, Patrícia H.; Silva, Margarida; Bellora, Nicolás; et al.; The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.; Multidisciplinary Digital Publishing Institute; Microorganisms; 8; 11; 11-2020; 1-152076-2607CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8111651info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/11/1651info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:36:55Zoai:ri.conicet.gov.ar:11336/183651instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:36:55.323CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
title The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
spellingShingle The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
David Palma, Márcia
ASTAXANTHIN
FUNGAL MAT GENES
PHAFFIA
PHYLOGENOMICS
YEAST TAXONOMY
title_short The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
title_full The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
title_fullStr The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
title_full_unstemmed The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
title_sort The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.
dc.creator.none.fl_str_mv David Palma, Márcia
Libkind Frati, Diego
Brito, Patrícia H.
Silva, Margarida
Bellora, Nicolás
Coelho, Marco A.
Heitman, Joseph
Gonçalves, Paula
Sampaio, José Paulo
author David Palma, Márcia
author_facet David Palma, Márcia
Libkind Frati, Diego
Brito, Patrícia H.
Silva, Margarida
Bellora, Nicolás
Coelho, Marco A.
Heitman, Joseph
Gonçalves, Paula
Sampaio, José Paulo
author_role author
author2 Libkind Frati, Diego
Brito, Patrícia H.
Silva, Margarida
Bellora, Nicolás
Coelho, Marco A.
Heitman, Joseph
Gonçalves, Paula
Sampaio, José Paulo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ASTAXANTHIN
FUNGAL MAT GENES
PHAFFIA
PHYLOGENOMICS
YEAST TAXONOMY
topic ASTAXANTHIN
FUNGAL MAT GENES
PHAFFIA
PHYLOGENOMICS
YEAST TAXONOMY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phaffia is an orange-colored basidiomycetous yeast genus of the order Cystofilobasidiales that contains a single species, P. rhodozyma. This species is the only fungus known to produce the economically relevant carotenoid astaxanthin. Although Phaffia was originally found in the Northern hemisphere, its diversity in the southern part of the globe has been shown to be much greater. Here we analyze the genomes of two Australasian lineages that are markedly distinct from P. rhodozyma. The two divergent lineages were investigated within a comprehensive phylogenomic study of representatives of the Cystofilobasidiales that supported the recognition of two novel Phaffia species, for which we propose the names of P. australis sp. nov. and P. tasmanica sp. nov. Comparative genomics and other analyses confirmed that the two new species have the typical Phaffia hallmark—the six genes necessary for the biosynthesis of astaxanthin could be retrieved from the draft genome sequences, and this carotenoid was detected in culture extracts. In addition, the organization of the mating-type (MAT) loci is similar to that of P. rhodozyma, with synteny throughout most regions. Moreover, cases of trans-specific polymorphism involving pheromone receptor genes and pheromone precursor proteins in the three Phaffia species, together with their shared homothallism, provide additional support for their classification in a single genus.
Fil: David Palma, Márcia. University of Duke; Estados Unidos. Universidade Nova de Lisboa; Portugal
Fil: Libkind Frati, Diego. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales. Universidad Nacional del Comahue. Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales; Argentina
Fil: Brito, Patrícia H.. Universidade Nova de Lisboa; Portugal
Fil: Silva, Margarida. Universidade Nova de Lisboa; Portugal
Fil: Bellora, Nicolás. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina
Fil: Coelho, Marco A.. Universidade Nova de Lisboa; Portugal
Fil: Heitman, Joseph. University of Duke; Estados Unidos
Fil: Gonçalves, Paula. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;
Fil: Sampaio, José Paulo. Faculdade de Ciencias E Tecnologia ; Universidade Nova de Lisboa;
description Phaffia is an orange-colored basidiomycetous yeast genus of the order Cystofilobasidiales that contains a single species, P. rhodozyma. This species is the only fungus known to produce the economically relevant carotenoid astaxanthin. Although Phaffia was originally found in the Northern hemisphere, its diversity in the southern part of the globe has been shown to be much greater. Here we analyze the genomes of two Australasian lineages that are markedly distinct from P. rhodozyma. The two divergent lineages were investigated within a comprehensive phylogenomic study of representatives of the Cystofilobasidiales that supported the recognition of two novel Phaffia species, for which we propose the names of P. australis sp. nov. and P. tasmanica sp. nov. Comparative genomics and other analyses confirmed that the two new species have the typical Phaffia hallmark—the six genes necessary for the biosynthesis of astaxanthin could be retrieved from the draft genome sequences, and this carotenoid was detected in culture extracts. In addition, the organization of the mating-type (MAT) loci is similar to that of P. rhodozyma, with synteny throughout most regions. Moreover, cases of trans-specific polymorphism involving pheromone receptor genes and pheromone precursor proteins in the three Phaffia species, together with their shared homothallism, provide additional support for their classification in a single genus.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/183651
David Palma, Márcia; Libkind Frati, Diego; Brito, Patrícia H.; Silva, Margarida; Bellora, Nicolás; et al.; The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.; Multidisciplinary Digital Publishing Institute; Microorganisms; 8; 11; 11-2020; 1-15
2076-2607
CONICET Digital
CONICET
url http://hdl.handle.net/11336/183651
identifier_str_mv David Palma, Márcia; Libkind Frati, Diego; Brito, Patrícia H.; Silva, Margarida; Bellora, Nicolás; et al.; The untapped Australasian diversity of astaxanthin-producing yeasts with biotechnological potential—Phaffia Australis sp. nov. and Phaffia tasmanica sp. nov.; Multidisciplinary Digital Publishing Institute; Microorganisms; 8; 11; 11-2020; 1-15
2076-2607
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/microorganisms8111651
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2607/8/11/1651
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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