Susceptibility of placental mitochondria to oxidative stress

Autores
Papa Gobbi, Rodrigo; Magnarelli, Gladis; Rovedatti, María Gabriela
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF.
Fil: Papa Gobbi, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Papa Gobbi, Rodrigo. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Papa Gobbi, Rodrigo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina.
Fil: Magnarelli, Gladis Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina
Fil: Rovedatti, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Rovedatti, María Gabriela. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Rovedatti, María Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
Fuente
Birth Defects Research. 2018, 110
Materia
Mitochondria
Placenta
Oxidative stress
Reactive oxygen species
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Digital Institucional (UNCo)
Institución
Universidad Nacional del Comahue
OAI Identificador
oai:rdi.uncoma.edu.ar:uncomaid/19298
Acceder
id RDIUNCO_b3bd7aaf69cca9ea2f837a9a829134c4
oai_identifier_str oai:rdi.uncoma.edu.ar:uncomaid/19298
network_acronym_str RDIUNCO
repository_id_str 7108
network_name_str Repositorio Digital Institucional (UNCo)
spelling Susceptibility of placental mitochondria to oxidative stressPapa Gobbi, RodrigoMagnarelli, GladisRovedatti, María GabrielaMitochondriaPlacentaOxidative stressReactive oxygen speciesCiencias de la Tierra y Medio AmbienteCiencias BiomédicasArtículosBackground: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF.Fil: Papa Gobbi, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Papa Gobbi, Rodrigo. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Papa Gobbi, Rodrigo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina.Fil: Magnarelli, Gladis Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; ArgentinaFil: Rovedatti, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Rovedatti, María Gabriela. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Rovedatti, María Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.John Wiley & Sons2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfpp. 1228-1232application/pdf2472-1727https://rdi.uncoma.edu.ar/handle/uncomaid/19298Birth Defects Research. 2018, 110reponame:Repositorio Digital Institucional (UNCo)instname:Universidad Nacional del ComahueengDOI: 10.1002/bdr2.1377https://ri.conicet.gov.ar/handle/11336/212681info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2026-05-14T12:31:39Zoai:rdi.uncoma.edu.ar:uncomaid/19298instacron:UNCoInstitucionalhttp://rdi.uncoma.edu.ar/Universidad públicaNo correspondehttp://rdi.uncoma.edu.ar/oaimirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:71082026-05-14 12:31:40.24Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahuefalse
dc.title.none.fl_str_mv Susceptibility of placental mitochondria to oxidative stress
title Susceptibility of placental mitochondria to oxidative stress
spellingShingle Susceptibility of placental mitochondria to oxidative stress
Papa Gobbi, Rodrigo
Mitochondria
Placenta
Oxidative stress
Reactive oxygen species
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
title_short Susceptibility of placental mitochondria to oxidative stress
title_full Susceptibility of placental mitochondria to oxidative stress
title_fullStr Susceptibility of placental mitochondria to oxidative stress
title_full_unstemmed Susceptibility of placental mitochondria to oxidative stress
title_sort Susceptibility of placental mitochondria to oxidative stress
dc.creator.none.fl_str_mv Papa Gobbi, Rodrigo
Magnarelli, Gladis
Rovedatti, María Gabriela
author Papa Gobbi, Rodrigo
author_facet Papa Gobbi, Rodrigo
Magnarelli, Gladis
Rovedatti, María Gabriela
author_role author
author2 Magnarelli, Gladis
Rovedatti, María Gabriela
author2_role author
author
dc.subject.none.fl_str_mv Mitochondria
Placenta
Oxidative stress
Reactive oxygen species
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
topic Mitochondria
Placenta
Oxidative stress
Reactive oxygen species
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
dc.description.none.fl_txt_mv Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF.
Fil: Papa Gobbi, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Papa Gobbi, Rodrigo. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Papa Gobbi, Rodrigo. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina.
Fil: Magnarelli, Gladis Griselda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Magnarelli, Gladis Griselda. Universidad Nacional del Comahue. Centro Regional Universitario Bariloche; Argentina
Fil: Rovedatti, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Rovedatti, María Gabriela. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Rovedatti, María Gabriela. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Biológica; Argentina.
description Background: Two different mitochondrial fractions (MFs) have been character- ized in the human placenta: the “light” and “heavy” fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a seri- ous threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and suscepti- bility to oxidative stress in both types of MFs. Methods: Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2O2. Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results: H2O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion: H2O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in pla- cental HMF and LMF performed in a cell-free assay. The small number of antioxi- dant system parameters measured did not allow detecting differences between HMF and LMF.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv 2472-1727
https://rdi.uncoma.edu.ar/handle/uncomaid/19298
identifier_str_mv 2472-1727
url https://rdi.uncoma.edu.ar/handle/uncomaid/19298
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv DOI: 10.1002/bdr2.1377
https://ri.conicet.gov.ar/handle/11336/212681
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
pp. 1228-1232
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Birth Defects Research. 2018, 110
reponame:Repositorio Digital Institucional (UNCo)
instname:Universidad Nacional del Comahue
reponame_str Repositorio Digital Institucional (UNCo)
collection Repositorio Digital Institucional (UNCo)
instname_str Universidad Nacional del Comahue
repository.name.fl_str_mv Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahue
repository.mail.fl_str_mv mirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.ar
_version_ 1865180428020744192
score 13.115601

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