Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy

Autores
Rodriguez, Piuque M.; Vera, Berta; Burgos, Carolina; Gimenez, Gustavo; Miglioranza, Karina S.B.; Ramirez, Cristina L.; Lavalle, Andrea; Ondarza, Paola M.; Guiñazú, Natalia L.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.
Fil: Rodriguez, Piuque M. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.
Fil: Rodriguez, Piuque M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina.
Fil: Vera, Berta. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Burgos, Carolina. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Gimenez, Gustavo. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.
Fil: Miglioranza, Karina S.B. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Miglioranza, Karina S.B. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.
Fil: Ramirez, Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química y Bioquímica; Argentina.
Fil: Lavalle, Andrea. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.
Fil: Ondarza, Paola M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Ondarza, Paola M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.
Fil: Giñazú, Natalia L. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.
Fil: Giñazú, Natalia L. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fuente
Ecotoxicology and Environmental Safety. Volume 298
Materia
Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
Ciencias Biomédicas
Artículos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Digital Institucional (UNCo)
Institución
Universidad Nacional del Comahue
OAI Identificador
oai:rdi.uncoma.edu.ar:uncomaid/19045
Acceder
id RDIUNCO_6d81844fce12d4f5b66a35766a29e94a
oai_identifier_str oai:rdi.uncoma.edu.ar:uncomaid/19045
network_acronym_str RDIUNCO
repository_id_str 7108
network_name_str Repositorio Digital Institucional (UNCo)
spelling Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancyRodriguez, Piuque M.Vera, BertaBurgos, CarolinaGimenez, GustavoMiglioranza, Karina S.B.Ramirez, Cristina L.Lavalle, AndreaOndarza, Paola M.Guiñazú, Natalia L.ChlorpyrifosPlacentaEsterasesEnvironmental exposureCarboxylesterasesParaoxonaseshttps://purl.org/becyt/ford/3.3Ciencias BiomédicasArtículosExposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.Fil: Rodriguez, Piuque M. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.Fil: Rodriguez, Piuque M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina.Fil: Vera, Berta. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Burgos, Carolina. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Fil: Gimenez, Gustavo. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.Fil: Miglioranza, Karina S.B. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Miglioranza, Karina S.B. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.Fil: Ramirez, Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química y Bioquímica; Argentina.Fil: Lavalle, Andrea. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.Fil: Ondarza, Paola M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.Fil: Ondarza, Paola M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.Fil: Giñazú, Natalia L. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.Fil: Giñazú, Natalia L. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.Elsevier2025-05-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfpp.1-13application/pdf0147-65131090-2414https://rdi.uncoma.edu.ar/handle/uncomaid/19045Ecotoxicology and Environmental Safety. Volume 298reponame:Repositorio Digital Institucional (UNCo)instname:Universidad Nacional del Comahueenghttps://doi.org/10.1016/j.ecoenv.2025.118285info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-10-16T10:05:38Zoai:rdi.uncoma.edu.ar:uncomaid/19045instacron:UNCoInstitucionalhttp://rdi.uncoma.edu.ar/Universidad públicaNo correspondehttp://rdi.uncoma.edu.ar/oaimirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:71082025-10-16 10:05:38.435Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahuefalse
dc.title.none.fl_str_mv Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
spellingShingle Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
Rodriguez, Piuque M.
Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
Ciencias Biomédicas
Artículos
title_short Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_full Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_fullStr Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_full_unstemmed Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
title_sort Expression of carboxylesterase and paraoxonase in the placenta and their association with chlorpyrifos exposure during pregnancy
dc.creator.none.fl_str_mv Rodriguez, Piuque M.
Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
author Rodriguez, Piuque M.
author_facet Rodriguez, Piuque M.
Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
author_role author
author2 Vera, Berta
Burgos, Carolina
Gimenez, Gustavo
Miglioranza, Karina S.B.
Ramirez, Cristina L.
Lavalle, Andrea
Ondarza, Paola M.
Guiñazú, Natalia L.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
Ciencias Biomédicas
Artículos
topic Chlorpyrifos
Placenta
Esterases
Environmental exposure
Carboxylesterases
Paraoxonases
Ciencias Biomédicas
Artículos
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
dc.description.none.fl_txt_mv Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.
Fil: Rodriguez, Piuque M. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.
Fil: Rodriguez, Piuque M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Vera, Berta. Universidad Nacional del Comahue. Facultad de Ciencias Médicas; Argentina.
Fil: Vera, Berta. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Burgos, Carolina. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
Fil: Gimenez, Gustavo. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.
Fil: Miglioranza, Karina S.B. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Miglioranza, Karina S.B. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.
Fil: Ramirez, Cristina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Departamento de Química y Bioquímica; Argentina.
Fil: Lavalle, Andrea. Universidad Nacional del Comahue. Facultad de Economía. Departamento de Estadística; Argentina.
Fil: Ondarza, Paola M. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil: Ondarza, Paola M. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Instituto de Investigaciones Marinas y Costeras. Laboratorio de Ecotoxicología y Contaminación Ambiental; Argentina.
Fil: Giñazú, Natalia L. Universidad Nacional del Comahue. Facultad de Ciencias del Ambiente y la Salud. Departamento de Ciencias del Ambiente; Argentina.
Fil: Giñazú, Natalia L. Consejo Nacional de Investigaciones Científicas y Tecnológicas. Centro de Investigaciones en Toxicología Ambiental y Agrobiotecnología del Comahue; Argentina.
description Exposure to environmental chemicals during pregnancy, including organophosphate pesticides, can affect the health of both the mother and the fetus, and have repercussions later in life. The present study aimed to determine whether the A-esterases paraoxonases (PON) and the B-esterases carboxylesterases (CES) are modulated in the placenta of pregnant women residing in an intensive pesticide use scenario. A total of 104 healthy pregnant women were recruited between 2018 and 2022 and were classified according to their residential settings in rural (RG) and urban (UG) groups. Chlorpyrifos (CP) level in the placenta was determined by GC-ECD, and confirmed by GC-MS. To analyze possible impacts in esterases, the CES and PON activity, mRNA transcript and protein expression levels were studied. Significantly higher CP levels were detected in RG vs UG. Also, CES activity determined with 1-naphthyl acetate substrate was significantly lower in RG vs UG. In contrast, PON arylesterase and lactonase activities were up modulated in RG vs UG. Likewise, mRNA transcript levels of CES1, CES2 and PON2 were upregulated in the RG along with increases in CES2 and PON2 protein expressions. Moreover, a positive significant correlation was determined between CP concentration and CES1 and CES2 mRNA levels. Rural samples showed elevated CP concentrations and alterations in esterases, which elucidate the impact of CP exposure in mRNA CES and PON regulation. These findings highlight the need for further investigation into the effects of pesticide exposure during pregnancy and to deepen the knowledge about the function that esterases play in the placenta.
publishDate 2025
dc.date.none.fl_str_mv 2025-05-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv 0147-6513
1090-2414
https://rdi.uncoma.edu.ar/handle/uncomaid/19045
identifier_str_mv 0147-6513
1090-2414
url https://rdi.uncoma.edu.ar/handle/uncomaid/19045
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.ecoenv.2025.118285
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
pp.1-13
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Ecotoxicology and Environmental Safety. Volume 298
reponame:Repositorio Digital Institucional (UNCo)
instname:Universidad Nacional del Comahue
reponame_str Repositorio Digital Institucional (UNCo)
collection Repositorio Digital Institucional (UNCo)
instname_str Universidad Nacional del Comahue
repository.name.fl_str_mv Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahue
repository.mail.fl_str_mv mirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.ar
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score 13.22299

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