Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives

Autores
Gramisci, Betina Rocío; Lutz, María Cecilia; Lopes, Christian Ariel; Sangorrin, Marcela Paula
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.
Fil: Gramisci, Betina Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Gramisci, Betina Rocío. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Lutz, María Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Sangorrin, Marcela Paula. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
Fuente
Biological Control 121 (2018)
Materia
Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Digital Institucional (UNCo)
Institución
Universidad Nacional del Comahue
OAI Identificador
oai:rdi.uncoma.edu.ar:uncomaid/19296
Acceder
id RDIUNCO_5245f589bc8c533bc1ad311088962ecd
oai_identifier_str oai:rdi.uncoma.edu.ar:uncomaid/19296
network_acronym_str RDIUNCO
repository_id_str 7108
network_name_str Repositorio Digital Institucional (UNCo)
spelling Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additivesGramisci, Betina RocíoLutz, María CeciliaLopes, Christian ArielSangorrin, Marcela PaulaPostharvest diseasesAntagonist yeastBiocontrolNutritional profileCiencias de la Tierra y Medio AmbienteCiencias BiomédicasArtículosVishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.Fil: Gramisci, Betina Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Gramisci, Betina Rocío. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Lutz, María Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Sangorrin, Marcela Paula. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.Elsevier2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfpp. 151-158application/pdf1049-9644https://rdi.uncoma.edu.ar/handle/uncomaid/19296Biological Control 121 (2018)reponame:Repositorio Digital Institucional (UNCo)instname:Universidad Nacional del Comahueenghttps://doi.org/10.1016/j.biocontrol.2018.03.001https://ri.conicet.gov.ar/handle/11336/89478info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2026-05-07T11:59:07Zoai:rdi.uncoma.edu.ar:uncomaid/19296instacron:UNCoInstitucionalhttp://rdi.uncoma.edu.ar/Universidad públicaNo correspondehttp://rdi.uncoma.edu.ar/oaimirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:71082026-05-07 11:59:07.909Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahuefalse
dc.title.none.fl_str_mv Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
spellingShingle Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
Gramisci, Betina Rocío
Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
title_short Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_full Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_fullStr Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_full_unstemmed Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
title_sort Enhancing the efficacy of yeast biocontrol agents against postharvest pathogens through nutrient profiling and the use of other additives
dc.creator.none.fl_str_mv Gramisci, Betina Rocío
Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
author Gramisci, Betina Rocío
author_facet Gramisci, Betina Rocío
Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
author_role author
author2 Lutz, María Cecilia
Lopes, Christian Ariel
Sangorrin, Marcela Paula
author2_role author
author
author
dc.subject.none.fl_str_mv Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
topic Postharvest diseases
Antagonist yeast
Biocontrol
Nutritional profile
Ciencias de la Tierra y Medio Ambiente
Ciencias Biomédicas
Artículos
dc.description.none.fl_txt_mv Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.
Fil: Gramisci, Betina Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Gramisci, Betina Rocío. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Lutz, María Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
Fil: Lopes, Christian Ariel. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina.
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Sangorrin, Marcela Paula. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina.
description Vishniacozyma victoriae and Pichia membranifaciens were selected in a previous work for their biocontrol effec- tiveness against Penicillium expansum and Botrytis cinerea, the causal agents of blue and grey mold of pear fruits. The objective of this study was to determine the effects of different nutrient or additives on the growth of the two antagonist yeasts and of the two fungal pathogens in order to develop a rational selection based on nutritional profiles to be used in biocontrol enhancement of decay in pear fruits. Twenty-six different nutrient sources including 18 amino acids, five sugars, three inorganic nitrogen and one iron source were tested in vitro for their effect on the growth of the two yeasts and the two pathogens. Nutrients that promoted the growth of the yeasts and inhibited the growth of pathogens were applied with the antagonist to wounded fruits to evaluate their effect on enhancing biocontrol in cold storage. In general, the effect of each additive was specific to the yeasts and pathogens used in the in situ assays. The combination of the yeast and some additives resulted in a sig- nificantly higher activity with respect to the single treatments applied separately, producing synergistic effects. The effect of the exogenous application of CaCl2 and chitosan together with the antagonist yeasts was also evaluated against the two pathogens. The most effective mixtures were the CaCl2 with the two antagonist yeasts. Our research demonstrates that manipulating the chemical environment by adding several amino acid and/or Cl2Ca results in improved antagonistic activity of Vishniacozyma victoriae and Pichia membranifaciens against two postharvest diseases of pear fruits.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv 1049-9644
https://rdi.uncoma.edu.ar/handle/uncomaid/19296
identifier_str_mv 1049-9644
url https://rdi.uncoma.edu.ar/handle/uncomaid/19296
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.biocontrol.2018.03.001
https://ri.conicet.gov.ar/handle/11336/89478
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
pp. 151-158
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biological Control 121 (2018)
reponame:Repositorio Digital Institucional (UNCo)
instname:Universidad Nacional del Comahue
reponame_str Repositorio Digital Institucional (UNCo)
collection Repositorio Digital Institucional (UNCo)
instname_str Universidad Nacional del Comahue
repository.name.fl_str_mv Repositorio Digital Institucional (UNCo) - Universidad Nacional del Comahue
repository.mail.fl_str_mv mirtha.mateo@biblioteca.uncoma.edu.ar; adriana.acuna@biblioteca.uncoma.edu.ar
_version_ 1864552333963165696
score 13.1485815

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