Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens

Autores
Lutz, María Cecilia; Lopes, Christian Ariel; Rodríguez, María Eugenia; Sosa, M. Cristina; Sangorrin, Marcela Paula
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The putative mechanisms of action involved in the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied in vitro and in situ assays. C. albidus YP commercial strain was also evaluated with comparative purposes. All yeast strains showed different mechanisms including: wound colonization, germination inhibition, biofilms formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, quitinase and glucanase activities). The relationship between strains (and their associated antagonist mechanisms) and the in situ antagonist activity was also evaluated and our results suggest that mechanisms such as hydrolytic enzymes production, the ability for wounds colonization, toxin killer production and germination inhibition are the most important for biocontrol activity. Multiple modes of action as documented in this study might explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease.
Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Rodríguez, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Sosa, M. Cristina. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Departamento de Biologia Aplicada; Argentina
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina
Materia
Postharvest Diseases
Mode of Action
Antagonist Yeast
Biocontrol
Pyrus Communis L
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11669
Acceder
id CONICETDig_0aad1b76d12ad8610721e8d390434be0
oai_identifier_str oai:ri.conicet.gov.ar:11336/11669
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogensLutz, María CeciliaLopes, Christian ArielRodríguez, María EugeniaSosa, M. CristinaSangorrin, Marcela PaulaPostharvest DiseasesMode of ActionAntagonist YeastBiocontrolPyrus Communis Lhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The putative mechanisms of action involved in the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied in vitro and in situ assays. C. albidus YP commercial strain was also evaluated with comparative purposes. All yeast strains showed different mechanisms including: wound colonization, germination inhibition, biofilms formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, quitinase and glucanase activities). The relationship between strains (and their associated antagonist mechanisms) and the in situ antagonist activity was also evaluated and our results suggest that mechanisms such as hydrolytic enzymes production, the ability for wounds colonization, toxin killer production and germination inhibition are the most important for biocontrol activity. Multiple modes of action as documented in this study might explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease.Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; ArgentinaFil: Rodríguez, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Medicina; ArgentinaFil: Sosa, M. Cristina. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Departamento de Biologia Aplicada; ArgentinaFil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; ArgentinaElsevier Science2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11669Lutz, María Cecilia; Lopes, Christian Ariel; Rodríguez, María Eugenia; Sosa, M. Cristina; Sangorrin, Marcela Paula; Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens; Elsevier Science; International Journal of Food Microbiology; 164; 2-3; 6-2013; 166-1720168-1605enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160513001797info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.ijfoodmicro.2013.04.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:58:56Zoai:ri.conicet.gov.ar:11336/11669instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:58:57.253CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
title Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
spellingShingle Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
Lutz, María Cecilia
Postharvest Diseases
Mode of Action
Antagonist Yeast
Biocontrol
Pyrus Communis L
title_short Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
title_full Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
title_fullStr Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
title_full_unstemmed Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
title_sort Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens
dc.creator.none.fl_str_mv Lutz, María Cecilia
Lopes, Christian Ariel
Rodríguez, María Eugenia
Sosa, M. Cristina
Sangorrin, Marcela Paula
author Lutz, María Cecilia
author_facet Lutz, María Cecilia
Lopes, Christian Ariel
Rodríguez, María Eugenia
Sosa, M. Cristina
Sangorrin, Marcela Paula
author_role author
author2 Lopes, Christian Ariel
Rodríguez, María Eugenia
Sosa, M. Cristina
Sangorrin, Marcela Paula
author2_role author
author
author
author
dc.subject.none.fl_str_mv Postharvest Diseases
Mode of Action
Antagonist Yeast
Biocontrol
Pyrus Communis L
topic Postharvest Diseases
Mode of Action
Antagonist Yeast
Biocontrol
Pyrus Communis L
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The putative mechanisms of action involved in the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied in vitro and in situ assays. C. albidus YP commercial strain was also evaluated with comparative purposes. All yeast strains showed different mechanisms including: wound colonization, germination inhibition, biofilms formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, quitinase and glucanase activities). The relationship between strains (and their associated antagonist mechanisms) and the in situ antagonist activity was also evaluated and our results suggest that mechanisms such as hydrolytic enzymes production, the ability for wounds colonization, toxin killer production and germination inhibition are the most important for biocontrol activity. Multiple modes of action as documented in this study might explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease.
Fil: Lutz, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Lopes, Christian Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Ciencias Agrarias; Argentina
Fil: Rodríguez, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Sosa, M. Cristina. Universidad Nacional del Comahue. Facultad de Cs.agrarias. Departamento de Biologia Aplicada; Argentina
Fil: Sangorrin, Marcela Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Patagonia Norte. Instituto de Investigación y Desarrollo En Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina. Universidad Nacional del Comahue. Facultad de Ingeniería; Argentina
description The putative mechanisms of action involved in the biocontrol capacity of four yeast strains (Cryptoccocus albidus NPCC 1248, Pichia membranifaciens NPCC 1250, Cryptoccocus victoriae NPCC 1263 and NPCC 1259) against Penicillium expansum and Botrytis cinerea were studied in vitro and in situ assays. C. albidus YP commercial strain was also evaluated with comparative purposes. All yeast strains showed different mechanisms including: wound colonization, germination inhibition, biofilms formation, secretion of killer toxins, competition for nutrient and secretion of hydrolytic enzymes (protease, quitinase and glucanase activities). The relationship between strains (and their associated antagonist mechanisms) and the in situ antagonist activity was also evaluated and our results suggest that mechanisms such as hydrolytic enzymes production, the ability for wounds colonization, toxin killer production and germination inhibition are the most important for biocontrol activity. Multiple modes of action as documented in this study might explain why P. membranifaciens NPCC 1250 and C. victoriae NPCC 1263 provided excellent control of postharvest pears disease.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11669
Lutz, María Cecilia; Lopes, Christian Ariel; Rodríguez, María Eugenia; Sosa, M. Cristina; Sangorrin, Marcela Paula; Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens; Elsevier Science; International Journal of Food Microbiology; 164; 2-3; 6-2013; 166-172
0168-1605
url http://hdl.handle.net/11336/11669
identifier_str_mv Lutz, María Cecilia; Lopes, Christian Ariel; Rodríguez, María Eugenia; Sosa, M. Cristina; Sangorrin, Marcela Paula; Efficacy and putative mode of action of native and commercial antagonistic yeasts against postharvest pear pathogens; Elsevier Science; International Journal of Food Microbiology; 164; 2-3; 6-2013; 166-172
0168-1605
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168160513001797
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.1016/j.ijfoodmicro.2013.04.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083130836910080
score 13.22299

Publicaciones similares