Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf

Autores
Álvarez, Fernando Andrés; Colombres, Gustavo Adolfo; Viqueira, Alejandro; Maldonado, Jorge Eduardo; Alvarellos, Emilio; Sambuelli, Rubén Horacio; Alvarellos, Teresita
Año de publicación
2010
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
Fil: Álvarez, Fernando Andrés. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Colombres, Gustavo Adolfo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Viqueira, Alejandro. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Maldonado, Jorge Eduardo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Alvarellos, Emilio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Sambuelli, Rubén Horacio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Alvarellos, Teresita. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fuente
Álvarez, Fernando Andrés ORCID: https://orcid.org/0000-0003-4644-5665 , Colombres, Gustavo Adolfo, Viqueira, Alejandro ORCID: https://orcid.org/0000-0001-7685-8853 , Maldonado, Jorge Eduardo, Alvarellos, Emilio, Sambuelli, Rubén Horacio and Alvarellos, Teresita ORCID: https://orcid.org/0000-0002-2618-7913 (2010) Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf. Acta Gastroenterológica Latinoamericana, 40 (4). pp. 339-346. ISSN 0300-9033
Materia
R Medicina (General)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Repositorio
Producción Académica (UCC)
Institución
Universidad Católica de Córdoba
OAI Identificador
oai:pa.bibdigital.uccor.edu.ar:3443
Acceder
id PAUCC_9fdcfaa47816fa964f1c44dff2eb6346
oai_identifier_str oai:pa.bibdigital.uccor.edu.ar:3443
network_acronym_str PAUCC
repository_id_str 2718
network_name_str Producción Académica (UCC)
spelling Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the grafÁlvarez, Fernando AndrésColombres, Gustavo AdolfoViqueira, AlejandroMaldonado, Jorge EduardoAlvarellos, EmilioSambuelli, Rubén HoracioAlvarellos, TeresitaR Medicina (General)Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.Fil: Álvarez, Fernando Andrés. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Colombres, Gustavo Adolfo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Viqueira, Alejandro. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Maldonado, Jorge Eduardo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Alvarellos, Emilio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Sambuelli, Rubén Horacio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaFil: Alvarellos, Teresita. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; ArgentinaSociedad Argentina de Gastroenterología2010-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf Álvarez, Fernando Andrés ORCID: https://orcid.org/0000-0003-4644-5665 <https://orcid.org/0000-0003-4644-5665>, Colombres, Gustavo Adolfo, Viqueira, Alejandro ORCID: https://orcid.org/0000-0001-7685-8853 <https://orcid.org/0000-0001-7685-8853>, Maldonado, Jorge Eduardo, Alvarellos, Emilio, Sambuelli, Rubén Horacio and Alvarellos, Teresita ORCID: https://orcid.org/0000-0002-2618-7913 <https://orcid.org/0000-0002-2618-7913> (2010) Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf. Acta Gastroenterológica Latinoamericana, 40 (4). pp. 339-346. ISSN 0300-9033 reponame:Producción Académica (UCC)instname:Universidad Católica de Córdobaspahttp://pa.bibdigital.ucc.edu.ar/3443/https://www.redalyc.org/articulo.oa?id=199317295009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es2025-09-29T14:29:33Zoai:pa.bibdigital.uccor.edu.ar:3443instacron:UCCInstitucionalhttp://pa.bibdigital.uccor.edu.ar/Universidad privadaNo correspondehttp://pa.bibdigital.uccor.edu.ar/cgi/oai2bibdir@uccor.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:27182025-09-29 14:29:34.115Producción Académica (UCC) - Universidad Católica de Córdobafalse
dc.title.none.fl_str_mv Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
spellingShingle Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
Álvarez, Fernando Andrés
R Medicina (General)
title_short Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_full Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_fullStr Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_full_unstemmed Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
title_sort Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf
dc.creator.none.fl_str_mv Álvarez, Fernando Andrés
Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
author Álvarez, Fernando Andrés
author_facet Álvarez, Fernando Andrés
Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
author_role author
author2 Colombres, Gustavo Adolfo
Viqueira, Alejandro
Maldonado, Jorge Eduardo
Alvarellos, Emilio
Sambuelli, Rubén Horacio
Alvarellos, Teresita
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv R Medicina (General)
topic R Medicina (General)
dc.description.none.fl_txt_mv Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
Fil: Álvarez, Fernando Andrés. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Colombres, Gustavo Adolfo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Viqueira, Alejandro. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Maldonado, Jorge Eduardo. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Alvarellos, Emilio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Sambuelli, Rubén Horacio. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
Fil: Alvarellos, Teresita. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina
description Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
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info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf
url http://pa.bibdigital.ucc.edu.ar/3443/1/A_%C3%81lvarez_Colombres_Viqueira_Maldonado_Alvarellos_Sambuelli_Alvarellos.pdf
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/3443/
https://www.redalyc.org/articulo.oa?id=199317295009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedad Argentina de Gastroenterología
publisher.none.fl_str_mv Sociedad Argentina de Gastroenterología
dc.source.none.fl_str_mv Álvarez, Fernando Andrés ORCID: https://orcid.org/0000-0003-4644-5665 <https://orcid.org/0000-0003-4644-5665>, Colombres, Gustavo Adolfo, Viqueira, Alejandro ORCID: https://orcid.org/0000-0001-7685-8853 <https://orcid.org/0000-0001-7685-8853>, Maldonado, Jorge Eduardo, Alvarellos, Emilio, Sambuelli, Rubén Horacio and Alvarellos, Teresita ORCID: https://orcid.org/0000-0002-2618-7913 <https://orcid.org/0000-0002-2618-7913> (2010) Splenic autoimplantation in omentum and stomach, hematoimmunological follow-up and B-cell repertoire in the graf. Acta Gastroenterológica Latinoamericana, 40 (4). pp. 339-346. ISSN 0300-9033
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reponame_str Producción Académica (UCC)
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instname_str Universidad Católica de Córdoba
repository.name.fl_str_mv Producción Académica (UCC) - Universidad Católica de Córdoba
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