The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets

Autores
Pereira Ramos, Pablo Iván; Flores Custódio, Márlon Grégori; Quispe Saji, Guadalupe del Rosario; Cardoso, Thiago; Lucchetti da Silva, Gisele; Braun, Graziela; Martins, Willames M.B.S.; Girardello, Raquel; Ribeiro de Vasconcelos1, Ana Tereza; Fernández, Elmer Andrés; Gales, Ana Cristina; Nicolás, Marisa Fabiana
Año de publicación
2016
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Background: The emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH), magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K. pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K. pneumoniae that could relate to surviving against the action of this antibiotic. Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.
Fil: Pereira Ramos, Pablo Iván. Laboratório Nacional de Computação Científica; Brasil
Fil: Flores Custódio, Márlon Grégori. Laboratório Nacional de Computação Científica; Brasil
Fil: Quispe Saji, Guadalupe del Rosario. Laboratório Nacional de Computação Científica; Brasil
Fil: Cardoso, Thiago. Laboratório Nacional de Computação Científica; Brasil
Fil: Lucchetti da Silva, Gisele. Laboratório Nacional de Computação Científica; Brasil
Fil: Braun, Graziela. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Martins, Willames M.B.S. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Girardello, Raquel. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Ribeiro de Vasconcelos, Ana Tereza. Laboratório Nacional de Computação Científica; Brasil
Fil: Fernández, Elmer Andrés. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina
Fil: Gales, Ana Cristina. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Nicolás, Marisa Fabiana. Laboratório Nacional de Computação Científica; Brasil
Fuente
Pereira Ramos, Pablo Iván, Flores Custódio, Márlon Grégori, Quispe Saji, Guadalupe del Rosario, Cardoso, Thiago, Lucchetti da Silva, Gisele, Braun, Graziela, Martins, Willames M.B.S., Girardello, Raquel, Ribeiro de Vasconcelos1, Ana Tereza, Fernández, Elmer Andrés ORCID: https://orcid.org/0000-0002-4711-8634 , Gales, Ana Cristina and Nicolás, Marisa Fabiana (2016) The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, 17 (S8). pp. 447-462. ISSN 1471-2164
Materia
Q Ciencia (General)
TA Ingeniería de asistencia técnica (General). Ingeniería Civil (General)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Repositorio
Producción Académica (UCC)
Institución
Universidad Católica de Córdoba
OAI Identificador
oai:pa.bibdigital.uccor.edu.ar:4229
Acceder
id PAUCC_8fe632770b63a5e7d4ec0538a97123f2
oai_identifier_str oai:pa.bibdigital.uccor.edu.ar:4229
network_acronym_str PAUCC
repository_id_str 2718
network_name_str Producción Académica (UCC)
spelling The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targetsPereira Ramos, Pablo IvánFlores Custódio, Márlon GrégoriQuispe Saji, Guadalupe del RosarioCardoso, ThiagoLucchetti da Silva, GiseleBraun, GrazielaMartins, Willames M.B.S.Girardello, RaquelRibeiro de Vasconcelos1, Ana TerezaFernández, Elmer AndrésGales, Ana CristinaNicolás, Marisa FabianaQ Ciencia (General)TA Ingeniería de asistencia técnica (General). Ingeniería Civil (General)Background: The emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH), magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K. pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K. pneumoniae that could relate to surviving against the action of this antibiotic. Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.Fil: Pereira Ramos, Pablo Iván. Laboratório Nacional de Computação Científica; BrasilFil: Flores Custódio, Márlon Grégori. Laboratório Nacional de Computação Científica; BrasilFil: Quispe Saji, Guadalupe del Rosario. Laboratório Nacional de Computação Científica; BrasilFil: Cardoso, Thiago. Laboratório Nacional de Computação Científica; BrasilFil: Lucchetti da Silva, Gisele. Laboratório Nacional de Computação Científica; BrasilFil: Braun, Graziela. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; BrasilFil: Martins, Willames M.B.S. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; BrasilFil: Girardello, Raquel. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; BrasilFil: Ribeiro de Vasconcelos, Ana Tereza. Laboratório Nacional de Computação Científica; BrasilFil: Fernández, Elmer Andrés. Universidad Católica de Córdoba. Facultad de Ingeniería; ArgentinaFil: Gales, Ana Cristina. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; BrasilFil: Nicolás, Marisa Fabiana. Laboratório Nacional de Computação Científica; BrasilBioMed Central Ltd2016-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://pa.bibdigital.ucc.edu.ar/4229/1/A_PereiraRamos.pdf Pereira Ramos, Pablo Iván, Flores Custódio, Márlon Grégori, Quispe Saji, Guadalupe del Rosario, Cardoso, Thiago, Lucchetti da Silva, Gisele, Braun, Graziela, Martins, Willames M.B.S., Girardello, Raquel, Ribeiro de Vasconcelos1, Ana Tereza, Fernández, Elmer Andrés ORCID: https://orcid.org/0000-0002-4711-8634 <https://orcid.org/0000-0002-4711-8634>, Gales, Ana Cristina and Nicolás, Marisa Fabiana (2016) The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, 17 (S8). pp. 447-462. ISSN 1471-2164 reponame:Producción Académica (UCC)instname:Universidad Católica de Córdobaspahttp://pa.bibdigital.ucc.edu.ar/4229/info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1186/s12864-016-3070-yinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es2025-11-06T10:09:42Zoai:pa.bibdigital.uccor.edu.ar:4229instacron:UCCInstitucionalhttp://pa.bibdigital.uccor.edu.ar/Universidad privadaNo correspondehttp://pa.bibdigital.uccor.edu.ar/cgi/oai2bibdir@uccor.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:27182025-11-06 10:09:42.971Producción Académica (UCC) - Universidad Católica de Córdobafalse
dc.title.none.fl_str_mv The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
spellingShingle The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
Pereira Ramos, Pablo Iván
Q Ciencia (General)
TA Ingeniería de asistencia técnica (General). Ingeniería Civil (General)
title_short The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_full The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_fullStr The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_full_unstemmed The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
title_sort The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets
dc.creator.none.fl_str_mv Pereira Ramos, Pablo Iván
Flores Custódio, Márlon Grégori
Quispe Saji, Guadalupe del Rosario
Cardoso, Thiago
Lucchetti da Silva, Gisele
Braun, Graziela
Martins, Willames M.B.S.
Girardello, Raquel
Ribeiro de Vasconcelos1, Ana Tereza
Fernández, Elmer Andrés
Gales, Ana Cristina
Nicolás, Marisa Fabiana
author Pereira Ramos, Pablo Iván
author_facet Pereira Ramos, Pablo Iván
Flores Custódio, Márlon Grégori
Quispe Saji, Guadalupe del Rosario
Cardoso, Thiago
Lucchetti da Silva, Gisele
Braun, Graziela
Martins, Willames M.B.S.
Girardello, Raquel
Ribeiro de Vasconcelos1, Ana Tereza
Fernández, Elmer Andrés
Gales, Ana Cristina
Nicolás, Marisa Fabiana
author_role author
author2 Flores Custódio, Márlon Grégori
Quispe Saji, Guadalupe del Rosario
Cardoso, Thiago
Lucchetti da Silva, Gisele
Braun, Graziela
Martins, Willames M.B.S.
Girardello, Raquel
Ribeiro de Vasconcelos1, Ana Tereza
Fernández, Elmer Andrés
Gales, Ana Cristina
Nicolás, Marisa Fabiana
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Q Ciencia (General)
TA Ingeniería de asistencia técnica (General). Ingeniería Civil (General)
topic Q Ciencia (General)
TA Ingeniería de asistencia técnica (General). Ingeniería Civil (General)
dc.description.none.fl_txt_mv Background: The emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH), magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K. pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K. pneumoniae that could relate to surviving against the action of this antibiotic. Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.
Fil: Pereira Ramos, Pablo Iván. Laboratório Nacional de Computação Científica; Brasil
Fil: Flores Custódio, Márlon Grégori. Laboratório Nacional de Computação Científica; Brasil
Fil: Quispe Saji, Guadalupe del Rosario. Laboratório Nacional de Computação Científica; Brasil
Fil: Cardoso, Thiago. Laboratório Nacional de Computação Científica; Brasil
Fil: Lucchetti da Silva, Gisele. Laboratório Nacional de Computação Científica; Brasil
Fil: Braun, Graziela. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Martins, Willames M.B.S. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Girardello, Raquel. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Ribeiro de Vasconcelos, Ana Tereza. Laboratório Nacional de Computação Científica; Brasil
Fil: Fernández, Elmer Andrés. Universidad Católica de Córdoba. Facultad de Ingeniería; Argentina
Fil: Gales, Ana Cristina. Laboratório Alerta. Division of Infectious Diseases, Department of Internal Medicine, Escola Paulista de Medicina/Universidade Federal de São Paulo; Brasil
Fil: Nicolás, Marisa Fabiana. Laboratório Nacional de Computação Científica; Brasil
description Background: The emergence of multidrug-resistant Klebsiella pneumoniae is a major public health concern. Many K. pneumoniae infections can only be treated when resorting to last-line drugs such as polymyxin B (PB). However, resistance to this antibiotic is also observed, although insufficient information is described on its mode of action as well as the mechanisms used by resistant bacteria to evade its effects. We aimed to study PB resistance and the influence of abiotic stresses in a clinical K. pneumoniae strain using whole transcriptome profiling. Results: We sequenced 12 cDNA libraries of K. pneumoniae Kp13 bacteria, from two biological replicates of the original strain Kp13 (Kp13) and five derivative strains: induced high-level PB resistance in acidic pH (Kp13pH), magnesium deprivation (Kp13Mg), high concentrations of calcium (Kp13Ca) and iron (Kp13Fe), and a control condition with PB (Kp13PolB). Our results show the involvement of multiple regulatory loci that differentially respond to each condition as well as a shared gene expression response elicited by PB treatment, and indicate the participation of two-regulatory components such as ArcA-ArcB, which could be involved in re-routing the K. pneumoniae metabolism following PB treatment. Modules of co-expressed genes could be determined, which correlated to growth in acid stress and PB exposure. We hypothesize that polymyxin B induces metabolic shifts in K. pneumoniae that could relate to surviving against the action of this antibiotic. Conclusions: We obtained whole transcriptome data for K. pneumoniae under different environmental conditions and PB treatment. Our results supports the notion that the K. pneumoniae response to PB exposure goes beyond damaged membrane reconstruction and involves recruitment of multiple gene modules and intracellular targets.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/4229/1/A_PereiraRamos.pdf
url http://pa.bibdigital.ucc.edu.ar/4229/1/A_PereiraRamos.pdf
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/4229/
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1186/s12864-016-3070-y
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central Ltd
publisher.none.fl_str_mv BioMed Central Ltd
dc.source.none.fl_str_mv Pereira Ramos, Pablo Iván, Flores Custódio, Márlon Grégori, Quispe Saji, Guadalupe del Rosario, Cardoso, Thiago, Lucchetti da Silva, Gisele, Braun, Graziela, Martins, Willames M.B.S., Girardello, Raquel, Ribeiro de Vasconcelos1, Ana Tereza, Fernández, Elmer Andrés ORCID: https://orcid.org/0000-0002-4711-8634 <https://orcid.org/0000-0002-4711-8634>, Gales, Ana Cristina and Nicolás, Marisa Fabiana (2016) The polymyxin B-induced transcriptomic response of a clinical, multidrug-resistant Klebsiella pneumoniae involves multiple regulatory elements and intracellular targets. BMC Genomics, 17 (S8). pp. 447-462. ISSN 1471-2164
reponame:Producción Académica (UCC)
instname:Universidad Católica de Córdoba
reponame_str Producción Académica (UCC)
collection Producción Académica (UCC)
instname_str Universidad Católica de Córdoba
repository.name.fl_str_mv Producción Académica (UCC) - Universidad Católica de Córdoba
repository.mail.fl_str_mv bibdir@uccor.edu.ar
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