Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina

Autores
Gertiser, María Laura; Giagante, E.; Sgattoni, E.; Basabe, N.; Rivero, Fernando David; Luján, Hugo Daniel; Occhionero, M.; Pannicia, L.; Visciarelli, E.; Costamagna, Sixto
Año de publicación
2010
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Introduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
Fil: Gertiser, María Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giagante, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
Fil: Sgattoni, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
Fil: Basabe, N. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rivero, Fernando David. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Luján, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Occhionero, M. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pannicia, L. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Visciarelli, E. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Costamagna, Sixto. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fuente
Gertiser, María Laura ORCID: https://orcid.org/0000-0001-6820-8690 , Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 , Occhionero, M., Pannicia, L., Visciarelli, E. and Costamagna, Sixto ORCID: https://orcid.org/0000-0001-8358-6155 (2010) Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina. Revista Argentina de Microbiología, 42 (2). pp. 122-125. ISSN 0325-7541
Materia
QD Química
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Repositorio
Producción Académica (UCC)
Institución
Universidad Católica de Córdoba
OAI Identificador
oai:pa.bibdigital.uccor.edu.ar:3442
Acceder
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oai_identifier_str oai:pa.bibdigital.uccor.edu.ar:3442
network_acronym_str PAUCC
repository_id_str 2718
network_name_str Producción Académica (UCC)
spelling Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, ArgentinaGertiser, María LauraGiagante, E.Sgattoni, E.Basabe, N.Rivero, Fernando DavidLuján, Hugo DanielOcchionero, M.Pannicia, L.Visciarelli, E.Costamagna, SixtoQD QuímicaIntroduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.Fil: Gertiser, María Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Giagante, E. MEGAVISIÓN. Centro Privado de Oftalmología; ArgentinaFil: Sgattoni, E. MEGAVISIÓN. Centro Privado de Oftalmología; ArgentinaFil: Basabe, N. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Rivero, Fernando David. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Luján, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Occhionero, M. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pannicia, L. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Visciarelli, E. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Costamagna, Sixto. Departamento de Biología, Bioquímica y Farmacia; ArgentinaAsociación Argentina de Microbiología2010-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf Gertiser, María Laura ORCID: https://orcid.org/0000-0001-6820-8690 <https://orcid.org/0000-0001-6820-8690>, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315>, Occhionero, M., Pannicia, L., Visciarelli, E. and Costamagna, Sixto ORCID: https://orcid.org/0000-0001-8358-6155 <https://orcid.org/0000-0001-8358-6155> (2010) Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina. Revista Argentina de Microbiología, 42 (2). pp. 122-125. ISSN 0325-7541 reponame:Producción Académica (UCC)instname:Universidad Católica de Córdobaspahttp://pa.bibdigital.ucc.edu.ar/3442/https://www.redalyc.org/articulo.oa?id=213014892011info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es2025-09-29T14:29:33Zoai:pa.bibdigital.uccor.edu.ar:3442instacron:UCCInstitucionalhttp://pa.bibdigital.uccor.edu.ar/Universidad privadaNo correspondehttp://pa.bibdigital.uccor.edu.ar/cgi/oai2bibdir@uccor.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:27182025-09-29 14:29:34.112Producción Académica (UCC) - Universidad Católica de Córdobafalse
dc.title.none.fl_str_mv Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
spellingShingle Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
Gertiser, María Laura
QD Química
title_short Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_full Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_fullStr Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_full_unstemmed Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
title_sort Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina
dc.creator.none.fl_str_mv Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
author Gertiser, María Laura
author_facet Gertiser, María Laura
Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
author_role author
author2 Giagante, E.
Sgattoni, E.
Basabe, N.
Rivero, Fernando David
Luján, Hugo Daniel
Occhionero, M.
Pannicia, L.
Visciarelli, E.
Costamagna, Sixto
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv QD Química
topic QD Química
dc.description.none.fl_txt_mv Introduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
Fil: Gertiser, María Laura. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giagante, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
Fil: Sgattoni, E. MEGAVISIÓN. Centro Privado de Oftalmología; Argentina
Fil: Basabe, N. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Rivero, Fernando David. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Luján, Hugo Daniel. Universidad Católica de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Occhionero, M. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pannicia, L. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Visciarelli, E. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Costamagna, Sixto. Departamento de Biología, Bioquímica y Farmacia; Argentina
description Introduction. Splenic autoimplantation appears to be the only alternative to preserve splenic tissue after splenectomy; however, its relevance is still controversial. We intended to study splenic autoimplantation in the greater omentum and stomach wall of rabbits and analyze its hematoimmunological performance and the preservation of original structures. Methods. New Zealand rabbits were divided in two groups: autoimplanted (A) (n=13) and splenectomized (S) (n=4). The animals of group A underwent autoimplantation of splenic fragments in the greater omentum and gastric wall. Both groups were evaluated by hemocytological tests, scintigraphy, immunoglobulin and C3 dosages, before the surgery and 2 and 4 months afterwards. After 4 months, the grafts were removed and histological examination and gen rearrangement of B-lymphocytes receptors by polymerase chain reaction (PCR) were performed to assess the cellular diversity of clones. Results. The histological analysis demonstrated the presence of splenic tissue in 10 of the 13 cases (77%) with evident size reduction. The gastric location did not develop complications and demonstrated higher morphological correspondence to the autoimplanted tissue. Both groups showed significant decrease of IgM and increase of C3, without considerable differences between both of them during follow up. From the 8 grafts studied with PCR, 3 cases presented polyclonality and 5 oligoclonality. Conclusions. The revascularized grafts evidenced splenic regenerating tissue, probably associated to the oligoclonality detected by PCR. Consequently, we consider that autoimplantation is a reasonable alternative for splenectomized patients, even though the stomach placement and the high frequency of oligoclonality justify further investigation.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
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info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf
url http://pa.bibdigital.ucc.edu.ar/3442/1/A_Gertiser_Giagante_Sgattoni_Basabe_Rivero_Luj%C3%A1n_Occhionero_Paniccia_Visciarelli_Costamagna.pdf
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv http://pa.bibdigital.ucc.edu.ar/3442/
https://www.redalyc.org/articulo.oa?id=213014892011
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv Gertiser, María Laura ORCID: https://orcid.org/0000-0001-6820-8690 <https://orcid.org/0000-0001-6820-8690>, Giagante, E., Sgattoni, E., Basabe, N., Rivero, Fernando David, Luján, Hugo Daniel ORCID: https://orcid.org/0000-0002-3797-8315 <https://orcid.org/0000-0002-3797-8315>, Occhionero, M., Pannicia, L., Visciarelli, E. and Costamagna, Sixto ORCID: https://orcid.org/0000-0001-8358-6155 <https://orcid.org/0000-0001-8358-6155> (2010) Queratitis por Acanthamoeba sp.: primer caso confirmado por aislamiento y tipificación molecular en Bahía Blanca, provincia de Buenos Aires, Argentina. Revista Argentina de Microbiología, 42 (2). pp. 122-125. ISSN 0325-7541
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reponame_str Producción Académica (UCC)
collection Producción Académica (UCC)
instname_str Universidad Católica de Córdoba
repository.name.fl_str_mv Producción Académica (UCC) - Universidad Católica de Córdoba
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