A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesiu...

Autores
Gualano, Leonardo D.; Moriconi, Jorge I.; Gabay, Gilad; Tranquilli, Gabriela; Pacheco, Pablo H.; Dubcovsky, Jorge; Santa-María, Guillermo E.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.
Instituto de Recursos Biológicos
Fil: Gualano, Leonardo D. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil Moriconi, Jorge I. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gabay, Gilad. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Pacheco, Pablo H. Universidad Nacional de San Luís. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luís (INQUISAL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Duvcoscky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fil: Santa María, Guillermo E. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Plant Physiology 303 : 154376. (December 2024)
Materia
Jasmonates
Magnesium
Rye
Wheat
Jasmonato
Magnesio
Centeno
Trigo
12-OXOPHYTODIENOATE REDUCTASE III
12-OXOFITODIENOATO REDUCTASA III
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/20751
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spelling A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supplyGualano, Leonardo D.Moriconi, Jorge I.Gabay, GiladTranquilli, GabrielaPacheco, Pablo H.Dubcovsky, JorgeSanta-María, Guillermo E.JasmonatesMagnesiumRyeWheatJasmonatoMagnesioCentenoTrigo12-OXOPHYTODIENOATE REDUCTASE III12-OXOFITODIENOATO REDUCTASA IIIWe previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.Instituto de Recursos BiológicosFil: Gualano, Leonardo D. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil Moriconi, Jorge I. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gabay, Gilad. University of California. Department of Plant Sciences; Estados UnidosFil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Pacheco, Pablo H. Universidad Nacional de San Luís. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luís (INQUISAL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Duvcoscky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados UnidosFil: Santa María, Guillermo E. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2024-12-26T12:03:12Z2024-12-26T12:03:12Z2024-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/20751https://www.sciencedirect.com/science/article/pii/S01761617240020740176-1617https://doi.org/10.1016/j.jplph.2024.154376Journal of Plant Physiology 303 : 154376. (December 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-18T10:09:53Zoai:localhost:20.500.12123/20751instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:09:53.362INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
spellingShingle A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
Gualano, Leonardo D.
Jasmonates
Magnesium
Rye
Wheat
Jasmonato
Magnesio
Centeno
Trigo
12-OXOPHYTODIENOATE REDUCTASE III
12-OXOFITODIENOATO REDUCTASA III
title_short A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_full A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_fullStr A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_full_unstemmed A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_sort A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
dc.creator.none.fl_str_mv Gualano, Leonardo D.
Moriconi, Jorge I.
Gabay, Gilad
Tranquilli, Gabriela
Pacheco, Pablo H.
Dubcovsky, Jorge
Santa-María, Guillermo E.
author Gualano, Leonardo D.
author_facet Gualano, Leonardo D.
Moriconi, Jorge I.
Gabay, Gilad
Tranquilli, Gabriela
Pacheco, Pablo H.
Dubcovsky, Jorge
Santa-María, Guillermo E.
author_role author
author2 Moriconi, Jorge I.
Gabay, Gilad
Tranquilli, Gabriela
Pacheco, Pablo H.
Dubcovsky, Jorge
Santa-María, Guillermo E.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Jasmonates
Magnesium
Rye
Wheat
Jasmonato
Magnesio
Centeno
Trigo
12-OXOPHYTODIENOATE REDUCTASE III
12-OXOFITODIENOATO REDUCTASA III
topic Jasmonates
Magnesium
Rye
Wheat
Jasmonato
Magnesio
Centeno
Trigo
12-OXOPHYTODIENOATE REDUCTASE III
12-OXOFITODIENOATO REDUCTASA III
dc.description.none.fl_txt_mv We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.
Instituto de Recursos Biológicos
Fil: Gualano, Leonardo D. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil Moriconi, Jorge I. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gabay, Gilad. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Pacheco, Pablo H. Universidad Nacional de San Luís. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luís (INQUISAL); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Duvcoscky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fil: Santa María, Guillermo E. Universidad Nacional de San Martín. Escuela de Bio y Nanotecnologías. Instituto Tecnológico Chascomús (INTECH); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale c ereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-26T12:03:12Z
2024-12-26T12:03:12Z
2024-11-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/20751
https://www.sciencedirect.com/science/article/pii/S0176161724002074
0176-1617
https://doi.org/10.1016/j.jplph.2024.154376
url http://hdl.handle.net/20.500.12123/20751
https://www.sciencedirect.com/science/article/pii/S0176161724002074
https://doi.org/10.1016/j.jplph.2024.154376
identifier_str_mv 0176-1617
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Plant Physiology 303 : 154376. (December 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.001348

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