A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesiu...

Autores: Gualano, Leonardo David; Moriconi, Jorge Ignacio; Gabay, Gilad; Tranquilli, Gabriela Edith; Pacheco, Pablo Hugo; Dubcovsky, Jorge; Santa Maria, Guillermo Esteban
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: conjunto de datos
Estado
Descripción: We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale cereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.
Fil: Gualano, Leonardo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Moriconi, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Gabay, Gilad. Department Of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Pacheco, Pablo Hugo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dubcovsky, Jorge. Department Of Plant Sciences; Estados Unidos
Fil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Nivel de accesibilidad: acceso abierto
Condiciones de uso: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
Institución: Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador: oai:ri.conicet.gov.ar:11336/246912

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spelling	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supplyGualano, Leonardo DavidMoriconi, Jorge IgnacioGabay, GiladTranquilli, Gabriela EdithPacheco, Pablo HugoDubcovsky, JorgeSanta Maria, Guillermo Estebanhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale cereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.Fil: Gualano, Leonardo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Moriconi, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Gabay, Gilad. Department Of Plant Sciences; Estados UnidosFil: Tranquilli, Gabriela Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; ArgentinaFil: Pacheco, Pablo Hugo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dubcovsky, Jorge. Department Of Plant Sciences; Estados UnidosFil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina2024info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/11336/246912Gualano, Leonardo David; Moriconi, Jorge Ignacio; Gabay, Gilad; Tranquilli, Gabriela Edith; Pacheco, Pablo Hugo; Dubcovsky, Jorge; Santa Maria, Guillermo Esteban; (2024): A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/246912CONICET DigitalCONICETenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:08:54Zoai:ri.conicet.gov.ar:11336/246912instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:08:54.726CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
spellingShingle	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply Gualano, Leonardo David
title_short	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_full	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_fullStr	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_full_unstemmed	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
title_sort	A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply
dc.creator.none.fl_str_mv	Gualano, Leonardo David Moriconi, Jorge Ignacio Gabay, Gilad Tranquilli, Gabriela Edith Pacheco, Pablo Hugo Dubcovsky, Jorge Santa Maria, Guillermo Esteban
author	Gualano, Leonardo David
author_facet	Gualano, Leonardo David Moriconi, Jorge Ignacio Gabay, Gilad Tranquilli, Gabriela Edith Pacheco, Pablo Hugo Dubcovsky, Jorge Santa Maria, Guillermo Esteban
author_role	author
author2	Moriconi, Jorge Ignacio Gabay, Gilad Tranquilli, Gabriela Edith Pacheco, Pablo Hugo Dubcovsky, Jorge Santa Maria, Guillermo Esteban
author2_role	author author author author author author
purl_subject.fl_str_mv	https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv	We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale cereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity. Fil: Gualano, Leonardo David. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Moriconi, Jorge Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Gabay, Gilad. Department Of Plant Sciences; Estados Unidos Fil: Tranquilli, Gabriela Edith. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina Fil: Pacheco, Pablo Hugo. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dubcovsky, Jorge. Department Of Plant Sciences; Estados Unidos Fil: Santa Maria, Guillermo Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description	We previously reported a structural rearrangement between wheat (Triticum aestivum) and rye (Secale cereale) chromosomes 1BS/1RS that increased the dosage of 12-OXOPHYTODIENOATE REDUCTASE III (OPRIII) genes involved in jasmonate biosynthesis (henceforth, 1RW line), and that drastically reduced primary root growth relative to a control line with the intact 1RS chromosome (henceforth, 1RS). In this study, we show that the increased gene-dosage of this region is associated with increases in the shoot-root partitioning of magnesium (Mg). Moreover, both a CRISPR-edited 1RW line with reduced OPRIII dosage and the 1RW line treated with the jasmonate biosynthesis inhibitor ibuprofen showed reduced differences in shoot-root Mg partitioning than 1RW. The observed differences in Mg partitioning between 1RS and 1RW plants occur over a wide range of external Mg supplies and imply opposite trends of Mg accumulation in roots and shoots. Furthermore, we show an association between the increase of shoot-root Mg partitioning and increased tolerance of the 1RW line to low levels of Mg supply. In summary, our results provide evidence of the role of the jasmonate pathway on the dynamics of Mg accumulation in roots and shoots, which correlates with the performance of wheat plants under conditions of Mg scarcity.
publishDate	2024
dc.date.none.fl_str_mv	2024
dc.type.none.fl_str_mv	info:ar-repo/semantics/conjuntoDeDatos v1.0 info:eu-repo/semantics/dataSet
format	dataSet
dc.identifier.none.fl_str_mv	http://hdl.handle.net/11336/246912 Gualano, Leonardo David; Moriconi, Jorge Ignacio; Gabay, Gilad; Tranquilli, Gabriela Edith; Pacheco, Pablo Hugo; Dubcovsky, Jorge; Santa Maria, Guillermo Esteban; (2024): A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/246912 CONICET Digital CONICET
url	http://hdl.handle.net/11336/246912
identifier_str_mv	Gualano, Leonardo David; Moriconi, Jorge Ignacio; Gabay, Gilad; Tranquilli, Gabriela Edith; Pacheco, Pablo Hugo; Dubcovsky, Jorge; Santa Maria, Guillermo Esteban; (2024): A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesium supply. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/246912 CONICET Digital CONICET
dc.language.none.fl_str_mv	eng
language	eng
dc.rights.none.fl_str_mv	info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv	openAccess
rights_invalid_str_mv	https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
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reponame_str	CONICET Digital (CONICET)
collection	CONICET Digital (CONICET)
instname_str	Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv	CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv	dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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A triplicated wheat-rye chromosome segment including several 12-OXOPHYTODIENOATE REDUCTASE III genes influences magnesium partitioning and impacts wheat performance at low magnesiu...

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