Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage

Autores
Lombrado, Verónica A.; Osorio, Sonia; Borsani, Julia; Lauxmann, Martín Alexander; Bustamante, Claudia Anabel; Budde, Claudio Olaf; Andreo, Carlos Santiago; Lara, María Valeria; Fernie, Alisdair R.; Drincovich, María Fabiana
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species.
EEA San Pedro
Fil: Lombardo, Verónica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Borsani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fuente
Plant Physiology 157 (4) : 1696-1710 (dec. 2011).
Materia
Durazno
Desarrollo Biológico
Crecimiento
Fruto
Metabolitos
Maduramiento
Metabolismo
Peaches
Biological Development
Growth
Fruit
Metabolites
Ripening
Metabolism
Desarrollo del Fruto
Crecimiento del Fruto
Perfil Metabólico
Fruit Development
Fruit Growth
Metabolic Profiling
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5310
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5310
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stageLombrado, Verónica A.Osorio, SoniaBorsani, JuliaLauxmann, Martín AlexanderBustamante, Claudia AnabelBudde, Claudio OlafAndreo, Carlos SantiagoLara, María ValeriaFernie, Alisdair R.Drincovich, María FabianaDuraznoDesarrollo BiológicoCrecimientoFrutoMetabolitosMaduramientoMetabolismoPeachesBiological DevelopmentGrowthFruitMetabolitesRipeningMetabolismDesarrollo del FrutoCrecimiento del FrutoPerfil MetabólicoFruit DevelopmentFruit GrowthMetabolic ProfilingFruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species.EEA San PedroFil: Lombardo, Verónica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; AlemaniaFil: Borsani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Fernie, Alisdair R. Max-Planck-Institute für Molekulare Pflanzenphysiologie; AlemaniaFil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina2019-06-13T13:34:11Z2019-06-13T13:34:11Z2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.plantphysiol.org/content/157/4/1696http://hdl.handle.net/20.500.12123/53100032-08891532-2548ehttps://doi.org/10.1104/pp.111.186064Plant Physiology 157 (4) : 1696-1710 (dec. 2011).reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:58Zoai:localhost:20.500.12123/5310instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:59.06INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
title Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
spellingShingle Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
Lombrado, Verónica A.
Durazno
Desarrollo Biológico
Crecimiento
Fruto
Metabolitos
Maduramiento
Metabolismo
Peaches
Biological Development
Growth
Fruit
Metabolites
Ripening
Metabolism
Desarrollo del Fruto
Crecimiento del Fruto
Perfil Metabólico
Fruit Development
Fruit Growth
Metabolic Profiling
title_short Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
title_full Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
title_fullStr Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
title_full_unstemmed Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
title_sort Metabolic profiling during peach fruit development and ripening reveals the metabolic networks that underpin each developmental stage
dc.creator.none.fl_str_mv Lombrado, Verónica A.
Osorio, Sonia
Borsani, Julia
Lauxmann, Martín Alexander
Bustamante, Claudia Anabel
Budde, Claudio Olaf
Andreo, Carlos Santiago
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author Lombrado, Verónica A.
author_facet Lombrado, Verónica A.
Osorio, Sonia
Borsani, Julia
Lauxmann, Martín Alexander
Bustamante, Claudia Anabel
Budde, Claudio Olaf
Andreo, Carlos Santiago
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author_role author
author2 Osorio, Sonia
Borsani, Julia
Lauxmann, Martín Alexander
Bustamante, Claudia Anabel
Budde, Claudio Olaf
Andreo, Carlos Santiago
Lara, María Valeria
Fernie, Alisdair R.
Drincovich, María Fabiana
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Durazno
Desarrollo Biológico
Crecimiento
Fruto
Metabolitos
Maduramiento
Metabolismo
Peaches
Biological Development
Growth
Fruit
Metabolites
Ripening
Metabolism
Desarrollo del Fruto
Crecimiento del Fruto
Perfil Metabólico
Fruit Development
Fruit Growth
Metabolic Profiling
topic Durazno
Desarrollo Biológico
Crecimiento
Fruto
Metabolitos
Maduramiento
Metabolismo
Peaches
Biological Development
Growth
Fruit
Metabolites
Ripening
Metabolism
Desarrollo del Fruto
Crecimiento del Fruto
Perfil Metabólico
Fruit Development
Fruit Growth
Metabolic Profiling
dc.description.none.fl_txt_mv Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species.
EEA San Pedro
Fil: Lombardo, Verónica A. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Osorio, Sonia. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Borsani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description Fruit from rosaceous species collectively display a great variety of flavors and textures as well as a generally high content of nutritionally beneficial metabolites. However, relatively little analysis of metabolic networks in rosaceous fruit has been reported. Among rosaceous species, peach (Prunus persica) has stone fruits composed of a juicy mesocarp and lignified endocarp. Here, peach mesocarp metabolic networks were studied across development using metabolomics and analysis of key regulatory enzymes. Principal component analysis of peach metabolic composition revealed clear metabolic shifts from early through late development stages and subsequently during postharvest ripening. Early developmental stages were characterized by a substantial decrease in protein abundance and high levels of bioactive polyphenols and amino acids, which are substrates for the phenylpropanoid and lignin pathways during stone hardening. Sucrose levels showed a large increase during development, reflecting translocation from the leaf, while the importance of galactinol and raffinose is also inferred. Our study further suggests that posttranscriptional mechanisms are key for metabolic regulation at early stages. In contrast to early developmental stages, a decrease in amino acid levels is coupled to an induction of transcripts encoding amino acid and organic acid catabolic enzymes during ripening. These data are consistent with the mobilization of amino acids to support respiration. In addition, sucrose cycling, suggested by the parallel increase of transcripts encoding sucrose degradative and synthetic enzymes, appears to operate during postharvest ripening. When taken together, these data highlight singular metabolic programs for peach development and may allow the identification of key factors related to agronomic traits of this important crop species.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
2019-06-13T13:34:11Z
2019-06-13T13:34:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.plantphysiol.org/content/157/4/1696
http://hdl.handle.net/20.500.12123/5310
0032-0889
1532-2548e
https://doi.org/10.1104/pp.111.186064
url http://www.plantphysiol.org/content/157/4/1696
http://hdl.handle.net/20.500.12123/5310
https://doi.org/10.1104/pp.111.186064
identifier_str_mv 0032-0889
1532-2548e
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Physiology 157 (4) : 1696-1710 (dec. 2011).
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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