Biotechnological applications of occlusion bodies of Baculoviruses

Autores
Lopez, Maria Gabriela; Diez, Michay; Alfonso, Victoria; Taboga, Oscar Alberto
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
Instituto de Biotecnología
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Applied microbiology and biotechnology 102 (16) : 6765–6774. (August 2018)
Materia
Baculovirus
Proteínas Recombinantes
Biotecnología
Recombinant Proteins
Biotechnology
Occlusion Bodies
BEVS
Polyhedrin
Cuerpos de Oclusión
Polihedrina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4456
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spelling Biotechnological applications of occlusion bodies of BaculovirusesLopez, Maria GabrielaDiez, MichayAlfonso, VictoriaTaboga, Oscar AlbertoBaculovirusProteínas RecombinantesBiotecnologíaRecombinant ProteinsBiotechnologyOcclusion BodiesBEVSPolyhedrinCuerpos de OclusiónPolihedrinaThe ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.Instituto de BiotecnologíaFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2019-02-18T13:15:59Z2019-02-18T13:15:59Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/44560175-75981432-0614 (Online)https://doi.org/10.1007/s00253-018-9130-2Applied microbiology and biotechnology 102 (16) : 6765–6774. (August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:48Zoai:localhost:20.500.12123/4456instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:49.245INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Biotechnological applications of occlusion bodies of Baculoviruses
title Biotechnological applications of occlusion bodies of Baculoviruses
spellingShingle Biotechnological applications of occlusion bodies of Baculoviruses
Lopez, Maria Gabriela
Baculovirus
Proteínas Recombinantes
Biotecnología
Recombinant Proteins
Biotechnology
Occlusion Bodies
BEVS
Polyhedrin
Cuerpos de Oclusión
Polihedrina
title_short Biotechnological applications of occlusion bodies of Baculoviruses
title_full Biotechnological applications of occlusion bodies of Baculoviruses
title_fullStr Biotechnological applications of occlusion bodies of Baculoviruses
title_full_unstemmed Biotechnological applications of occlusion bodies of Baculoviruses
title_sort Biotechnological applications of occlusion bodies of Baculoviruses
dc.creator.none.fl_str_mv Lopez, Maria Gabriela
Diez, Michay
Alfonso, Victoria
Taboga, Oscar Alberto
author Lopez, Maria Gabriela
author_facet Lopez, Maria Gabriela
Diez, Michay
Alfonso, Victoria
Taboga, Oscar Alberto
author_role author
author2 Diez, Michay
Alfonso, Victoria
Taboga, Oscar Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Baculovirus
Proteínas Recombinantes
Biotecnología
Recombinant Proteins
Biotechnology
Occlusion Bodies
BEVS
Polyhedrin
Cuerpos de Oclusión
Polihedrina
topic Baculovirus
Proteínas Recombinantes
Biotecnología
Recombinant Proteins
Biotechnology
Occlusion Bodies
BEVS
Polyhedrin
Cuerpos de Oclusión
Polihedrina
dc.description.none.fl_txt_mv The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
Instituto de Biotecnología
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Diez, Michay. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The ability of Baculoviruses to hyper-express very late genes as polyhedrin, the major component of occlusion bodies (OBs) or polyhedra, has allowed the evolution of a system of great utility for biotechnology. The main function of polyhedra in nature is to protect Baculovirus in the environment. The possibility of incorporating foreign proteins into the crystal by fusing them to polyhedrin (POLH) opened novel potential biotechnological uses. In this review, we summarize different applications of Baculovirus chimeric OBs. Basically, the improvement of protein expression and purification with POLH as a fusion partner; the use of recombinant polyhedra as immunogens and antigens, and the incorporation of proteins into polyhedra to improve Baculoviruses as bioinsecticides. The results obtained in each area and the future trends in these topics are also discussed.
publishDate 2018
dc.date.none.fl_str_mv 2018-08
2019-02-18T13:15:59Z
2019-02-18T13:15:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4456
0175-7598
1432-0614 (Online)
https://doi.org/10.1007/s00253-018-9130-2
url http://hdl.handle.net/20.500.12123/4456
https://doi.org/10.1007/s00253-018-9130-2
identifier_str_mv 0175-7598
1432-0614 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Applied microbiology and biotechnology 102 (16) : 6765–6774. (August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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