Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development

Autores
Youngjun Mo; Vanzetti, Leonardo Sebastian; Hale, Iago; Spagnolo, Emiliano J.; Guidobaldi, Fabio; Al-Oboudi, Jassim; Odle, Natalie; Pearce, Stephen; Helguera, Marcelo; Dubcovsky, Jorge
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere). Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments.
EEA Marcos Juárez
Fil: Youngjun, Mo. University of California. Department of Plant Sciences; Estados Unidos. Rural Development Administration. National Institute of Crop Science, Wanju; Corea del Sur
Fil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hale, Iago. University of New Hampshire. Department of Agriculture, Nutrition, and Food Systems; Estados Unidos
Fil: Spagnolo, Emiliano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Guidobaldi, Fabio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Al-Oboudi, Jassim. University of California. Department of Plant Sciences; Estados Unidos
Fil: Odle, Natalie. University of California. Department of Plant Sciences; Estados Unidos
Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos
Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fuente
Theoretical and applied genetics 1-15. (2018)
Materia
Trigo
Triticum Aestivum
Loci
Espigas
Altura
Genes
Etapas de Desarrollo de la Planta
Plant Developmental Stages
Height
Spikes
Wheat
Rht25
Cromosoma 6AS
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2836
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2836
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike developmentYoungjun MoVanzetti, Leonardo SebastianHale, IagoSpagnolo, Emiliano J.Guidobaldi, FabioAl-Oboudi, JassimOdle, NataliePearce, StephenHelguera, MarceloDubcovsky, JorgeTrigoTriticum AestivumLociEspigasAlturaGenesEtapas de Desarrollo de la PlantaPlant Developmental StagesHeightSpikesWheatRht25Cromosoma 6ASUnderstanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere). Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments.EEA Marcos JuárezFil: Youngjun, Mo. University of California. Department of Plant Sciences; Estados Unidos. Rural Development Administration. National Institute of Crop Science, Wanju; Corea del SurFil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hale, Iago. University of New Hampshire. Department of Agriculture, Nutrition, and Food Systems; Estados UnidosFil: Spagnolo, Emiliano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Guidobaldi, Fabio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Al-Oboudi, Jassim. University of California. Department of Plant Sciences; Estados UnidosFil: Odle, Natalie. University of California. Department of Plant Sciences; Estados UnidosFil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados UnidosFil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos2018-07-20T13:06:06Z2018-07-20T13:06:06Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2836https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas0040-57521432-2242 (Online)https://doi.org/10.1007/s00122-018-3130-6Theoretical and applied genetics 1-15. (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:21Zoai:localhost:20.500.12123/2836instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:22.314INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
title Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
spellingShingle Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
Youngjun Mo
Trigo
Triticum Aestivum
Loci
Espigas
Altura
Genes
Etapas de Desarrollo de la Planta
Plant Developmental Stages
Height
Spikes
Wheat
Rht25
Cromosoma 6AS
title_short Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
title_full Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
title_fullStr Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
title_full_unstemmed Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
title_sort Identification and characterization of Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development
dc.creator.none.fl_str_mv Youngjun Mo
Vanzetti, Leonardo Sebastian
Hale, Iago
Spagnolo, Emiliano J.
Guidobaldi, Fabio
Al-Oboudi, Jassim
Odle, Natalie
Pearce, Stephen
Helguera, Marcelo
Dubcovsky, Jorge
author Youngjun Mo
author_facet Youngjun Mo
Vanzetti, Leonardo Sebastian
Hale, Iago
Spagnolo, Emiliano J.
Guidobaldi, Fabio
Al-Oboudi, Jassim
Odle, Natalie
Pearce, Stephen
Helguera, Marcelo
Dubcovsky, Jorge
author_role author
author2 Vanzetti, Leonardo Sebastian
Hale, Iago
Spagnolo, Emiliano J.
Guidobaldi, Fabio
Al-Oboudi, Jassim
Odle, Natalie
Pearce, Stephen
Helguera, Marcelo
Dubcovsky, Jorge
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trigo
Triticum Aestivum
Loci
Espigas
Altura
Genes
Etapas de Desarrollo de la Planta
Plant Developmental Stages
Height
Spikes
Wheat
Rht25
Cromosoma 6AS
topic Trigo
Triticum Aestivum
Loci
Espigas
Altura
Genes
Etapas de Desarrollo de la Planta
Plant Developmental Stages
Height
Spikes
Wheat
Rht25
Cromosoma 6AS
dc.description.none.fl_txt_mv Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere). Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments.
EEA Marcos Juárez
Fil: Youngjun, Mo. University of California. Department of Plant Sciences; Estados Unidos. Rural Development Administration. National Institute of Crop Science, Wanju; Corea del Sur
Fil: Vanzetti, Leonardo Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hale, Iago. University of New Hampshire. Department of Agriculture, Nutrition, and Food Systems; Estados Unidos
Fil: Spagnolo, Emiliano J. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Guidobaldi, Fabio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; Argentina
Fil: Al-Oboudi, Jassim. University of California. Department of Plant Sciences; Estados Unidos
Fil: Odle, Natalie. University of California. Department of Plant Sciences; Estados Unidos
Fil: Pearce, Stephen. Colorado State University. Department of Soil and Crop Sciences; Estados Unidos
Fil: Helguera, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
description Understanding genes regulating wheat plant height is important to optimize harvest index and maximize grain yield. In modern wheat varieties grown under high-input conditions, the gibberellin-insensitive semi-dwarfing alleles Rht-B1b and Rht-D1b have been used extensively to confer lodging tolerance and improve harvest index. However, negative pleiotropic effects of these alleles (e.g., poor seedling emergence and reduced biomass) can cause yield losses in hot and dry environments. As part of current efforts to diversify the dwarfing alleles used in wheat breeding, we identified a quantitative trait locus (QHt.ucw-6AS) affecting plant height in the proximal region of chromosome arm 6AS (< 0.4 cM from the centromere). Using a large segregating population (~ 2800 gametes) and extensive progeny tests (70–93 plants per recombinant family), we mapped QHt.ucw-6AS as a Mendelian locus to a 0.2 cM interval (144.0–148.3 Mb, IWGSC Ref Seq v1.0) and show that it is different from Rht18. QHt.ucw-6AS is officially designated as Rht25, with Rht25a representing the height-increasing allele and Rht25b the dwarfing allele. The average dwarfing effect of Rht25b was found to be approximately half of the effect observed for Rht-B1b and Rht-D1b, and the effect is greater in the presence of the height-increasing Rht-B1a and Rht-D1a alleles than in the presence of the dwarfing alleles. Rht25b is gibberellin-sensitive and shows significant pleiotropic effects on coleoptile length, heading date, spike length, spikelet number, spikelet density, and grain weight. Rht25 represents a new alternative dwarfing locus that should be evaluated for its potential to improve wheat yield in different environments.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-20T13:06:06Z
2018-07-20T13:06:06Z
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2836
https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas
0040-5752
1432-2242 (Online)
https://doi.org/10.1007/s00122-018-3130-6
url http://hdl.handle.net/20.500.12123/2836
https://link.springer.com/article/10.1007/s00122-018-3130-6#citeas
https://doi.org/10.1007/s00122-018-3130-6
identifier_str_mv 0040-5752
1432-2242 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Theoretical and applied genetics 1-15. (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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