Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)

Autores
Zhang, Junli; LI, Chengxia; Zhang, Wenjun; Zhang, Xiaoqin; Mo, Youngjun; Tranquilli, Gabriela; Vanzetti, Leonardo Sebastian; Dubcovsky, Jorge
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.
Fil: Zhang, Junli. University of California. Department of Plant Sciences; Estados Unidos
Fil: Li, Chengxia. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhang, Wenjun. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhang, Xiaoqin. University of California. Department of Plant Sciences; Estados Unidos
Fil: Mo, Youngjun. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Vanzetti, Leonardo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
Fuente
PNAS 120 (19) : e2300203120 (May 2023)
Materia
Plant Height
Wheat
Altura de la Planta
Trigo
DELLA
PLATZ
Variación Natural
Natural Variation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/15250
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spelling Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)Zhang, JunliLI, ChengxiaZhang, WenjunZhang, XiaoqinMo, YoungjunTranquilli, GabrielaVanzetti, Leonardo SebastianDubcovsky, JorgePlant HeightWheatAltura de la PlantaTrigoDELLAPLATZVariación NaturalNatural VariationPlant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.Fil: Zhang, Junli. University of California. Department of Plant Sciences; Estados UnidosFil: Li, Chengxia. University of California. Department of Plant Sciences; Estados UnidosFil: Zhang, Wenjun. University of California. Department of Plant Sciences; Estados UnidosFil: Zhang, Xiaoqin. University of California. Department of Plant Sciences; Estados UnidosFil: Mo, Youngjun. University of California. Department of Plant Sciences; Estados UnidosFil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Vanzetti, Leonardo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; ArgentinaFil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados UnidosNational Academy of Sciences2023-09-19T17:13:04Z2023-09-19T17:13:04Z2023-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15250https://www.pnas.org/doi/10.1073/pnas.23002031200027-8424https://doi.org/10.1073/pnas.2300203120PNAS 120 (19) : e2300203120 (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:06Zoai:localhost:20.500.12123/15250instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:07.116INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
spellingShingle Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
Zhang, Junli
Plant Height
Wheat
Altura de la Planta
Trigo
DELLA
PLATZ
Variación Natural
Natural Variation
title_short Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_full Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_fullStr Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_full_unstemmed Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
title_sort Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)
dc.creator.none.fl_str_mv Zhang, Junli
LI, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela
Vanzetti, Leonardo Sebastian
Dubcovsky, Jorge
author Zhang, Junli
author_facet Zhang, Junli
LI, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela
Vanzetti, Leonardo Sebastian
Dubcovsky, Jorge
author_role author
author2 LI, Chengxia
Zhang, Wenjun
Zhang, Xiaoqin
Mo, Youngjun
Tranquilli, Gabriela
Vanzetti, Leonardo Sebastian
Dubcovsky, Jorge
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Plant Height
Wheat
Altura de la Planta
Trigo
DELLA
PLATZ
Variación Natural
Natural Variation
topic Plant Height
Wheat
Altura de la Planta
Trigo
DELLA
PLATZ
Variación Natural
Natural Variation
dc.description.none.fl_txt_mv Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.
Fil: Zhang, Junli. University of California. Department of Plant Sciences; Estados Unidos
Fil: Li, Chengxia. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhang, Wenjun. University of California. Department of Plant Sciences; Estados Unidos
Fil: Zhang, Xiaoqin. University of California. Department of Plant Sciences; Estados Unidos
Fil: Mo, Youngjun. University of California. Department of Plant Sciences; Estados Unidos
Fil: Tranquilli, Gabriela E. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Vanzetti, Leonardo Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez. Grupo Biotecnología y Recursos Genéticos; Argentina
Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos
description Plant height is an important agronomic trait with a significant impact on grain yield, as demonstrated by the positive effect of the REDUCED HEIGHT (RHT) dwarfing alleles (Rht1b) on lodging and harvest index in the “Green Revolution” wheat vari eties. However, these gibberellic acid (GA)-insensitive alleles also reduce coleoptile length, biomass production, and yield potential in some environments, triggering the search for alternative GA-sensitive dwarfing genes. Here we report the identification, validation, and characterization of the gene underlying the GA-sensitive dwarfing locus RHT25 in wheat. This gene, designated as PLATZ-A1 (TraesCS6A02G156600), is expressed mainly in the elongating stem and developing spike and encodes a plant-specific AT-rich sequence- and zinc-binding protein (PLATZ). Natural and induced loss-of-function mutations in PLATZ-A1 reduce plant height and its over expression increases plant height, demonstrating that PLATZ-A1 is the causative gene of RHT25. PLATZ-A1 and RHT1 show a significant genetic interaction on plant height, and their encoded proteins interact with each other in yeast and wheat protoplasts. These results suggest that PLATZ1 can modulate the effect of DELLA on wheat plant height. We identified four natural truncation mutations and one promoter insertion in PLATZ-A1 that are more frequent in modern varieties than in landraces, suggesting positive selection during wheat breeding. These mutations can be used to fine-tune wheat plant height and, in combination with other GA-sensitive dwarfing genes, to replace the GA-insensitive Rht1b alleles and search for grain yield improvements beyond those of the Green Revolution varieties.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-19T17:13:04Z
2023-09-19T17:13:04Z
2023-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15250
https://www.pnas.org/doi/10.1073/pnas.2300203120
0027-8424
https://doi.org/10.1073/pnas.2300203120
url http://hdl.handle.net/20.500.12123/15250
https://www.pnas.org/doi/10.1073/pnas.2300203120
https://doi.org/10.1073/pnas.2300203120
identifier_str_mv 0027-8424
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv PNAS 120 (19) : e2300203120 (May 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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