Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissue...
- Autores
- Rodríguez, Carlos E.; Bustamante, Claudia Anabel; Budde, Claudio Olaf; Müller, Gabriela Leticia; Drincovich, María Fabiana; Lara, María Valeria
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Peach (Prunus persica) is an important economically temperate fruit. The development follows double sigmoid curve with four phases (S1–S4). We centered our work in the early development. In addition to S1, we studied the very early stage (E) characterized by the lag zone of the exponential growing phase S1, and the second stage (S2) when the pit starts hardening. “Dixiland” peach fruit were collected at 9 (E), 29 (S1), and 53 (S2) days after flowering (DAF) and endocarp and mesocarp were separated. There was a pronounced decrease in total protein content along development in both tissues. Quantitative proteomic allowed the identification of changes in protein profiles across development and revealed the main biochemical pathways sustaining tissue differentiation. Protein metabolism was the category most represented among differentially proteins in all tissues and stages. The decrease in protein synthesis machinery observed during development would be responsible of the protein fall, rather than a proteolytic process; and reduced protein synthesis during early development would reroute cell resources to lignin biosynthesis. These changes were accompanied by net decrease in total amino acids in E1–S1 and increase in S1–S2 transitions. Amino acid profiling, showed Asn parallels this trend. Concerted changes in Asn and in enzymes involved in its metabolism reveal that increased synthesis and decreased catabolism of Asn may conduct to an Asn increase during very early development and that the β-Cyano-Alanine synthase/β-Cyano-Alanine hydratase could be the pathway for Asn synthesis in “Dixiland” peach fruit. Additionally, photosynthetic machinery decays during early development in mesocarp and endocarp. Proteins related to photosynthesis are found to a higher extent in mesocarp than in endocarp. We conclude mesocarpic photosynthesis is possible to occur early on the development, first providing both carbon and reductive power and latter only reductive power. Together with proteomic, histological tests and anatomical analysis help to provide information about changes and differences in cells and cell-walls in both tissues. Collectively, this work represents the first approach in building protein databases during peach fruit development focusing on endocarp and mesocarp tissues and provides novel insights into the biology of peach fruit development preceding pit hardening.
EEA San Pedro
Fil: Rodríguez, Carlos E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Müller, Gabriela Leticia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina - Fuente
- Frontiers in Plant Science 10 : 715 (june 2019)
- Materia
-
Durazno
Prunus persica
Lignificación
Fruto
Endocarpio
Mesocarpio
Asparagina
Desarrollo Biológico
Peaches
Lignification
Fruit
Endocarp
Mesocarp
Asparagine
Biological Development
Desarrollo del Fruto
β–cianoalanina hydratasa
β–cianoalanina sintasa
Fruit Development
β–cyanoalanine hydratase
β–cyanoalanine synthase - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5259
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Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissuesRodríguez, Carlos E.Bustamante, Claudia AnabelBudde, Claudio OlafMüller, Gabriela LeticiaDrincovich, María FabianaLara, María ValeriaDuraznoPrunus persicaLignificaciónFrutoEndocarpioMesocarpioAsparaginaDesarrollo BiológicoPeachesLignificationFruitEndocarpMesocarpAsparagineBiological DevelopmentDesarrollo del Frutoβ–cianoalanina hydratasaβ–cianoalanina sintasaFruit Developmentβ–cyanoalanine hydrataseβ–cyanoalanine synthasePeach (Prunus persica) is an important economically temperate fruit. The development follows double sigmoid curve with four phases (S1–S4). We centered our work in the early development. In addition to S1, we studied the very early stage (E) characterized by the lag zone of the exponential growing phase S1, and the second stage (S2) when the pit starts hardening. “Dixiland” peach fruit were collected at 9 (E), 29 (S1), and 53 (S2) days after flowering (DAF) and endocarp and mesocarp were separated. There was a pronounced decrease in total protein content along development in both tissues. Quantitative proteomic allowed the identification of changes in protein profiles across development and revealed the main biochemical pathways sustaining tissue differentiation. Protein metabolism was the category most represented among differentially proteins in all tissues and stages. The decrease in protein synthesis machinery observed during development would be responsible of the protein fall, rather than a proteolytic process; and reduced protein synthesis during early development would reroute cell resources to lignin biosynthesis. These changes were accompanied by net decrease in total amino acids in E1–S1 and increase in S1–S2 transitions. Amino acid profiling, showed Asn parallels this trend. Concerted changes in Asn and in enzymes involved in its metabolism reveal that increased synthesis and decreased catabolism of Asn may conduct to an Asn increase during very early development and that the β-Cyano-Alanine synthase/β-Cyano-Alanine hydratase could be the pathway for Asn synthesis in “Dixiland” peach fruit. Additionally, photosynthetic machinery decays during early development in mesocarp and endocarp. Proteins related to photosynthesis are found to a higher extent in mesocarp than in endocarp. We conclude mesocarpic photosynthesis is possible to occur early on the development, first providing both carbon and reductive power and latter only reductive power. Together with proteomic, histological tests and anatomical analysis help to provide information about changes and differences in cells and cell-walls in both tissues. Collectively, this work represents the first approach in building protein databases during peach fruit development focusing on endocarp and mesocarp tissues and provides novel insights into the biology of peach fruit development preceding pit hardening.EEA San PedroFil: Rodríguez, Carlos E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Müller, Gabriela Leticia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina2019-06-05T17:26:37Z2019-06-05T17:26:37Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5259https://www.frontiersin.org/articles/10.3389/fpls.2019.00715/full1664-462Xhttps://doi.org/10.3389/fpls.2019.00715Frontiers in Plant Science 10 : 715 (june 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:33Zoai:localhost:20.500.12123/5259instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:33.613INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
title |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
spellingShingle |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues Rodríguez, Carlos E. Durazno Prunus persica Lignificación Fruto Endocarpio Mesocarpio Asparagina Desarrollo Biológico Peaches Lignification Fruit Endocarp Mesocarp Asparagine Biological Development Desarrollo del Fruto β–cianoalanina hydratasa β–cianoalanina sintasa Fruit Development β–cyanoalanine hydratase β–cyanoalanine synthase |
title_short |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
title_full |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
title_fullStr |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
title_full_unstemmed |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
title_sort |
Peach fruit development: a comparative proteomic study between endocarp and mesocarp at very early stages underpins the main differential biochemical processes between these tissues |
dc.creator.none.fl_str_mv |
Rodríguez, Carlos E. Bustamante, Claudia Anabel Budde, Claudio Olaf Müller, Gabriela Leticia Drincovich, María Fabiana Lara, María Valeria |
author |
Rodríguez, Carlos E. |
author_facet |
Rodríguez, Carlos E. Bustamante, Claudia Anabel Budde, Claudio Olaf Müller, Gabriela Leticia Drincovich, María Fabiana Lara, María Valeria |
author_role |
author |
author2 |
Bustamante, Claudia Anabel Budde, Claudio Olaf Müller, Gabriela Leticia Drincovich, María Fabiana Lara, María Valeria |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Durazno Prunus persica Lignificación Fruto Endocarpio Mesocarpio Asparagina Desarrollo Biológico Peaches Lignification Fruit Endocarp Mesocarp Asparagine Biological Development Desarrollo del Fruto β–cianoalanina hydratasa β–cianoalanina sintasa Fruit Development β–cyanoalanine hydratase β–cyanoalanine synthase |
topic |
Durazno Prunus persica Lignificación Fruto Endocarpio Mesocarpio Asparagina Desarrollo Biológico Peaches Lignification Fruit Endocarp Mesocarp Asparagine Biological Development Desarrollo del Fruto β–cianoalanina hydratasa β–cianoalanina sintasa Fruit Development β–cyanoalanine hydratase β–cyanoalanine synthase |
dc.description.none.fl_txt_mv |
Peach (Prunus persica) is an important economically temperate fruit. The development follows double sigmoid curve with four phases (S1–S4). We centered our work in the early development. In addition to S1, we studied the very early stage (E) characterized by the lag zone of the exponential growing phase S1, and the second stage (S2) when the pit starts hardening. “Dixiland” peach fruit were collected at 9 (E), 29 (S1), and 53 (S2) days after flowering (DAF) and endocarp and mesocarp were separated. There was a pronounced decrease in total protein content along development in both tissues. Quantitative proteomic allowed the identification of changes in protein profiles across development and revealed the main biochemical pathways sustaining tissue differentiation. Protein metabolism was the category most represented among differentially proteins in all tissues and stages. The decrease in protein synthesis machinery observed during development would be responsible of the protein fall, rather than a proteolytic process; and reduced protein synthesis during early development would reroute cell resources to lignin biosynthesis. These changes were accompanied by net decrease in total amino acids in E1–S1 and increase in S1–S2 transitions. Amino acid profiling, showed Asn parallels this trend. Concerted changes in Asn and in enzymes involved in its metabolism reveal that increased synthesis and decreased catabolism of Asn may conduct to an Asn increase during very early development and that the β-Cyano-Alanine synthase/β-Cyano-Alanine hydratase could be the pathway for Asn synthesis in “Dixiland” peach fruit. Additionally, photosynthetic machinery decays during early development in mesocarp and endocarp. Proteins related to photosynthesis are found to a higher extent in mesocarp than in endocarp. We conclude mesocarpic photosynthesis is possible to occur early on the development, first providing both carbon and reductive power and latter only reductive power. Together with proteomic, histological tests and anatomical analysis help to provide information about changes and differences in cells and cell-walls in both tissues. Collectively, this work represents the first approach in building protein databases during peach fruit development focusing on endocarp and mesocarp tissues and provides novel insights into the biology of peach fruit development preceding pit hardening. EEA San Pedro Fil: Rodríguez, Carlos E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Müller, Gabriela Leticia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina |
description |
Peach (Prunus persica) is an important economically temperate fruit. The development follows double sigmoid curve with four phases (S1–S4). We centered our work in the early development. In addition to S1, we studied the very early stage (E) characterized by the lag zone of the exponential growing phase S1, and the second stage (S2) when the pit starts hardening. “Dixiland” peach fruit were collected at 9 (E), 29 (S1), and 53 (S2) days after flowering (DAF) and endocarp and mesocarp were separated. There was a pronounced decrease in total protein content along development in both tissues. Quantitative proteomic allowed the identification of changes in protein profiles across development and revealed the main biochemical pathways sustaining tissue differentiation. Protein metabolism was the category most represented among differentially proteins in all tissues and stages. The decrease in protein synthesis machinery observed during development would be responsible of the protein fall, rather than a proteolytic process; and reduced protein synthesis during early development would reroute cell resources to lignin biosynthesis. These changes were accompanied by net decrease in total amino acids in E1–S1 and increase in S1–S2 transitions. Amino acid profiling, showed Asn parallels this trend. Concerted changes in Asn and in enzymes involved in its metabolism reveal that increased synthesis and decreased catabolism of Asn may conduct to an Asn increase during very early development and that the β-Cyano-Alanine synthase/β-Cyano-Alanine hydratase could be the pathway for Asn synthesis in “Dixiland” peach fruit. Additionally, photosynthetic machinery decays during early development in mesocarp and endocarp. Proteins related to photosynthesis are found to a higher extent in mesocarp than in endocarp. We conclude mesocarpic photosynthesis is possible to occur early on the development, first providing both carbon and reductive power and latter only reductive power. Together with proteomic, histological tests and anatomical analysis help to provide information about changes and differences in cells and cell-walls in both tissues. Collectively, this work represents the first approach in building protein databases during peach fruit development focusing on endocarp and mesocarp tissues and provides novel insights into the biology of peach fruit development preceding pit hardening. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-06-05T17:26:37Z 2019-06-05T17:26:37Z 2019-06 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/5259 https://www.frontiersin.org/articles/10.3389/fpls.2019.00715/full 1664-462X https://doi.org/10.3389/fpls.2019.00715 |
url |
http://hdl.handle.net/20.500.12123/5259 https://www.frontiersin.org/articles/10.3389/fpls.2019.00715/full https://doi.org/10.3389/fpls.2019.00715 |
identifier_str_mv |
1664-462X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
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Frontiers in Plant Science 10 : 715 (june 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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