Characterization of grapevine wood microbiome through a metatranscriptomic approach

Autores
Paolinelli, Marcos; Escoriaza, Maria Georgina; Cesari, Cecilia Alicia; Garcia Lampasona, Sandra Claudia; Hernandez Martinez, Rufina
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease, but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected. Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification for specific taxa.
EEA Mendoza
Fil: Paolinelli, Marcos. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Cesari, Cecilia Alicia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias, Instituto de Biología Agrícola de Mendoza (IBAM), Mendoza, Argentina
Fil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza; Argentina
Fil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE); México
Fuente
Microbial Ecology (30 June 2021)
Materia
Vid
Enfermedades Fungosas
Microorganismos
ADN
Phellinus
Basidiomycota
Grapevines
Fungal Diseases
Microorganisms
DNA Sequence
Hoja de Malvón
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Characterization of grapevine wood microbiome through a metatranscriptomic approachPaolinelli, MarcosEscoriaza, Maria GeorginaCesari, Cecilia AliciaGarcia Lampasona, Sandra ClaudiaHernandez Martinez, RufinaVidEnfermedades FungosasMicroorganismosADNPhellinusBasidiomycotaGrapevinesFungal DiseasesMicroorganismsDNA SequenceHoja de MalvónGrapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease, but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected. Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification for specific taxa.EEA MendozaFil: Paolinelli, Marcos. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Escoriaza, Maria Georgina. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Cesari, Cecilia Alicia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias, Instituto de Biología Agrícola de Mendoza (IBAM), Mendoza, ArgentinaFil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza; ArgentinaFil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE); MéxicoSpringer2021-07-06T17:38:32Z2021-07-06T17:38:32Z2021-06-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9743https://link.springer.com/article/10.1007/s00248-021-01801-z0095-36281432-184Xhttps://doi.org/10.1007/s00248-021-01801-zMicrobial Ecology (30 June 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/MZASJ-1251101/AR./Desarrollo territorial del oasis norte de Mendoza.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:16Zoai:localhost:20.500.12123/9743instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:16.81INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of grapevine wood microbiome through a metatranscriptomic approach
title Characterization of grapevine wood microbiome through a metatranscriptomic approach
spellingShingle Characterization of grapevine wood microbiome through a metatranscriptomic approach
Paolinelli, Marcos
Vid
Enfermedades Fungosas
Microorganismos
ADN
Phellinus
Basidiomycota
Grapevines
Fungal Diseases
Microorganisms
DNA Sequence
Hoja de Malvón
title_short Characterization of grapevine wood microbiome through a metatranscriptomic approach
title_full Characterization of grapevine wood microbiome through a metatranscriptomic approach
title_fullStr Characterization of grapevine wood microbiome through a metatranscriptomic approach
title_full_unstemmed Characterization of grapevine wood microbiome through a metatranscriptomic approach
title_sort Characterization of grapevine wood microbiome through a metatranscriptomic approach
dc.creator.none.fl_str_mv Paolinelli, Marcos
Escoriaza, Maria Georgina
Cesari, Cecilia Alicia
Garcia Lampasona, Sandra Claudia
Hernandez Martinez, Rufina
author Paolinelli, Marcos
author_facet Paolinelli, Marcos
Escoriaza, Maria Georgina
Cesari, Cecilia Alicia
Garcia Lampasona, Sandra Claudia
Hernandez Martinez, Rufina
author_role author
author2 Escoriaza, Maria Georgina
Cesari, Cecilia Alicia
Garcia Lampasona, Sandra Claudia
Hernandez Martinez, Rufina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Vid
Enfermedades Fungosas
Microorganismos
ADN
Phellinus
Basidiomycota
Grapevines
Fungal Diseases
Microorganisms
DNA Sequence
Hoja de Malvón
topic Vid
Enfermedades Fungosas
Microorganismos
ADN
Phellinus
Basidiomycota
Grapevines
Fungal Diseases
Microorganisms
DNA Sequence
Hoja de Malvón
dc.description.none.fl_txt_mv Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease, but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected. Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification for specific taxa.
EEA Mendoza
Fil: Paolinelli, Marcos. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Escoriaza, Maria Georgina. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Cesari, Cecilia Alicia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias, Instituto de Biología Agrícola de Mendoza (IBAM), Mendoza, Argentina
Fil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza; Argentina
Fil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE); México
description Grapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM) significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease, but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected. Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification for specific taxa.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-06T17:38:32Z
2021-07-06T17:38:32Z
2021-06-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9743
https://link.springer.com/article/10.1007/s00248-021-01801-z
0095-3628
1432-184X
https://doi.org/10.1007/s00248-021-01801-z
url http://hdl.handle.net/20.500.12123/9743
https://link.springer.com/article/10.1007/s00248-021-01801-z
https://doi.org/10.1007/s00248-021-01801-z
identifier_str_mv 0095-3628
1432-184X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/MZASJ-1251101/AR./Desarrollo territorial del oasis norte de Mendoza.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Microbial Ecology (30 June 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619155354943488
score 12.559606

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