Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection

Autores
Pinto, Luiz R.; Bampi, Daiana; Silva, Talita N.; Edwards Molina, Juan Pablo; Kitajima, Elliot W.; Rezende, Jorge A.; Bergamin Filho, Armando
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic.
EEA Balcarce
Fil: Pinto, Luiz R. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Bampi, Daiana. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Silva, Talita N. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Edwards Molina, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Kitajima, Elliot W. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Rezende, Jorge A. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Bergamin Filho, Armando. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fuente
Tropical Plant Pathology 46 : 156-162 (March 2021)
Materia
Crinivirus
Papa
Tomate
Virus de las Plantas
Clorosis
Potatoes
Tomatoes
Plant Viruses
Chlorosis
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13456
Acceder
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network_name_str INTA Digital (INTA)
spelling Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infectionPinto, Luiz R.Bampi, DaianaSilva, Talita N.Edwards Molina, Juan PabloKitajima, Elliot W.Rezende, Jorge A.Bergamin Filho, ArmandoCrinivirusPapaTomateVirus de las PlantasClorosisPotatoesTomatoesPlant VirusesChlorosisTomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic.EEA BalcarceFil: Pinto, Luiz R. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Fil: Bampi, Daiana. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Fil: Silva, Talita N. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Fil: Edwards Molina, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Kitajima, Elliot W. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Fil: Rezende, Jorge A. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Fil: Bergamin Filho, Armando. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.Springer2022-11-28T10:40:59Z2022-11-28T10:40:59Z2021-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13456https://link.springer.com/article/10.1007/s40858-020-00379-01983-2052https://doi.org/10.1007/s40858-020-00379-0Tropical Plant Pathology 46 : 156-162 (March 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:38Zoai:localhost:20.500.12123/13456instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:38.999INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
title Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
spellingShingle Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
Pinto, Luiz R.
Crinivirus
Papa
Tomate
Virus de las Plantas
Clorosis
Potatoes
Tomatoes
Plant Viruses
Chlorosis
title_short Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
title_full Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
title_fullStr Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
title_full_unstemmed Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
title_sort Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection
dc.creator.none.fl_str_mv Pinto, Luiz R.
Bampi, Daiana
Silva, Talita N.
Edwards Molina, Juan Pablo
Kitajima, Elliot W.
Rezende, Jorge A.
Bergamin Filho, Armando
author Pinto, Luiz R.
author_facet Pinto, Luiz R.
Bampi, Daiana
Silva, Talita N.
Edwards Molina, Juan Pablo
Kitajima, Elliot W.
Rezende, Jorge A.
Bergamin Filho, Armando
author_role author
author2 Bampi, Daiana
Silva, Talita N.
Edwards Molina, Juan Pablo
Kitajima, Elliot W.
Rezende, Jorge A.
Bergamin Filho, Armando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Crinivirus
Papa
Tomate
Virus de las Plantas
Clorosis
Potatoes
Tomatoes
Plant Viruses
Chlorosis
topic Crinivirus
Papa
Tomate
Virus de las Plantas
Clorosis
Potatoes
Tomatoes
Plant Viruses
Chlorosis
dc.description.none.fl_txt_mv Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic.
EEA Balcarce
Fil: Pinto, Luiz R. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Bampi, Daiana. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Silva, Talita N. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Edwards Molina, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Kitajima, Elliot W. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Rezende, Jorge A. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
Fil: Bergamin Filho, Armando. Universidade de São Paulo. Departamento de Fitopatologia e Nematologia; Brasil.
description Tomato chlorosis virus (ToCV) is a whitefly-transmitted crinivirus that causes yield losses, mainly in tomato (Solanum lycopersicum) and potato (S. tuberosum) crops. In this work, a polyclonal antiserum for detecting the virus using a dot-blot immunoassay was developed and the responses of crinivirus-infected potato genotypes were evaluated. The virus was purified using infected tomato leaves and the antiserum was obtained by intramuscular injection of purified viral preparations in rabbit. Tomato and potato tissue samples were used to validate the polyclonal antiserum for detection of ToCV, previously analyzed by RT-PCR. A total of 81 tomato and potato samples were analyzed by RT-PCR and dot-blot immunoassay (DBIA). All samples were positive in RT-PCR, but three of them did not react in DBIA, showing of 96.3% efficiency compared with that in RT-PCR. All potato genotypes inoculated with ToCV by Bemisia tabaci MEAM1 were susceptible to infection. Potato plants of cv. Camila infected by ToCV showed the lowest virus titer and were asymptomatic.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-10
2022-11-28T10:40:59Z
2022-11-28T10:40:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13456
https://link.springer.com/article/10.1007/s40858-020-00379-0
1983-2052
https://doi.org/10.1007/s40858-020-00379-0
url http://hdl.handle.net/20.500.12123/13456
https://link.springer.com/article/10.1007/s40858-020-00379-0
https://doi.org/10.1007/s40858-020-00379-0
identifier_str_mv 1983-2052
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical Plant Pathology 46 : 156-162 (March 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341405591076864
score 12.623145

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