Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease

Autores
Novello, María Angelina; Bustamante, Claudia Anabel; Svetaz, Laura; Goldy, Camila; Valentini, Gabriel Hugo; Drincovich, María Fabiana; Brotman, Yadiv; Fernie, Alisdair R.; Lara, María Valeria
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route.
EEA San Pedro, INTA
Fil: Novello, María Angelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Bustamante, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Svetaz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Goldy, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Brotman, Yariv. Tel Aviv University. The George S. Wise Faculty of Life Sciences. School of Plant Sciences and Food Security; Israel
Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fuente
Plant, Cell & Environment : e15210 (October 2024)
Materia
Durazno
Prunus persica
Genética
Genómica
Asparagina
Glicosidos Cianogénicos
Metaboloma
Proteómica
Taphrina deformans
Peaches
Genetics
Genomics
Asparagine
Cyanogenic Glucosides
Metabolome
Proteomics
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/19816
Acceder
id INTADig_a0fc3141feb099879f3441a17a88532a
oai_identifier_str oai:localhost:20.500.12123/19816
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl diseaseNovello, María AngelinaBustamante, Claudia AnabelSvetaz, LauraGoldy, CamilaValentini, Gabriel HugoDrincovich, María FabianaBrotman, YadivFernie, Alisdair R.Lara, María ValeriaDuraznoPrunus persicaGenéticaGenómicaAsparaginaGlicosidos CianogénicosMetabolomaProteómicaTaphrina deformansPeachesGeneticsGenomicsAsparagineCyanogenic GlucosidesMetabolomeProteomicsPeach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route.EEA San Pedro, INTAFil: Novello, María Angelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Bustamante, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Svetaz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Goldy, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaFil: Brotman, Yariv. Tel Aviv University. The George S. Wise Faculty of Life Sciences. School of Plant Sciences and Food Security; IsraelFil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; AlemaniaFil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); ArgentinaWiley2024-10-22T12:26:58Z2024-10-22T12:26:58Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/19816https://onlinelibrary.wiley.com/doi/10.1111/pce.15210Novello, M.A., Bustamante, C.A., Svetaz, L.A., Goldy, C., Valentini, G.H., Drincovich, M.F., Brotman, Y., Fernie, A.R. and Lara, M.V. (2024), Integrated Metabolomic, Lipidomic and Proteomic Analysis Define the Metabolic Changes Occurring in Curled Areas in Leaves With Leaf Peach Curl Disease. Plant, Cell & Environment. https://doi.org/10.1111/pce.152101365-30400140-7791https://doi.org/10.1111/pce.15210Plant, Cell & Environment : e15210 (October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I125-001, Mejoramiento genético, caracterización y uso de variabilidad con aplicación de herramientas biotecnológicas en cultivos frutalesinfo:eu-repograntAgreement/INTA/2023-PE-L01-I105, Generación de conocimientos, tecnologías e innovaciones para una fruticultura sostenible adaptadas al riesgo ambiental y a la mecanizacióninfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:33Zoai:localhost:20.500.12123/19816instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:33.564INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
title Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
spellingShingle Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
Novello, María Angelina
Durazno
Prunus persica
Genética
Genómica
Asparagina
Glicosidos Cianogénicos
Metaboloma
Proteómica
Taphrina deformans
Peaches
Genetics
Genomics
Asparagine
Cyanogenic Glucosides
Metabolome
Proteomics
title_short Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
title_full Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
title_fullStr Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
title_full_unstemmed Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
title_sort Integrated metabolomic, lipidomic and proteomic analysis define the metabolic changes occurring in curled areas in leaves with leaf peach curl disease
dc.creator.none.fl_str_mv Novello, María Angelina
Bustamante, Claudia Anabel
Svetaz, Laura
Goldy, Camila
Valentini, Gabriel Hugo
Drincovich, María Fabiana
Brotman, Yadiv
Fernie, Alisdair R.
Lara, María Valeria
author Novello, María Angelina
author_facet Novello, María Angelina
Bustamante, Claudia Anabel
Svetaz, Laura
Goldy, Camila
Valentini, Gabriel Hugo
Drincovich, María Fabiana
Brotman, Yadiv
Fernie, Alisdair R.
Lara, María Valeria
author_role author
author2 Bustamante, Claudia Anabel
Svetaz, Laura
Goldy, Camila
Valentini, Gabriel Hugo
Drincovich, María Fabiana
Brotman, Yadiv
Fernie, Alisdair R.
Lara, María Valeria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Durazno
Prunus persica
Genética
Genómica
Asparagina
Glicosidos Cianogénicos
Metaboloma
Proteómica
Taphrina deformans
Peaches
Genetics
Genomics
Asparagine
Cyanogenic Glucosides
Metabolome
Proteomics
topic Durazno
Prunus persica
Genética
Genómica
Asparagina
Glicosidos Cianogénicos
Metaboloma
Proteómica
Taphrina deformans
Peaches
Genetics
Genomics
Asparagine
Cyanogenic Glucosides
Metabolome
Proteomics
dc.description.none.fl_txt_mv Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route.
EEA San Pedro, INTA
Fil: Novello, María Angelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Bustamante, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Svetaz, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Goldy, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
Fil: Brotman, Yariv. Tel Aviv University. The George S. Wise Faculty of Life Sciences. School of Plant Sciences and Food Security; Israel
Fil: Fernie, Alisdair R. Max-Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina
description Peach Leaf Curl Disease, caused by the fungus Taphrina deformans, is characterized by reddish hypertrophic and hyperplasic leaf areas. To comprehend the biochemical imbalances caused by the disease an integrated approach including metabolomics, lipidomics, proteomics and complementary biochemical techniques was undertaken. Symptomatic and asymptomatic areas were dissected from leaves with increasing extension of the disease. A differential metabolic behaviour was identified in symptomatic areas with respect to either asymptomatic areas or healthy leaves. Symptomatic areas showed an altered chloroplastic functioning and composition which differs from the typical senescence process and includes decrease in the photosynthetic machinery, alteration in plastidic lipids, and decreased starch, carotenoid and chlorophyll biosynthesis. In symptomatic areas, alteration in redox-homeostasis proteins and in triacylglycerols content, peroxidation and double bond index were observed. Proteomic data revealed induction of host enzymes involved in auxin and jasmonate biosynthesis together with up-regulation of phenylpropanoid and mevalonate pathways and down-regulation of the plastidic methylerythritol phosphate route. Amino acid pools were affected, with up-regulation of proteins involved in asparagine synthesis. Overall, we conclude that curled areas exhibited a metabolic shift towards functioning as a sink tissue importing sugars and producing energy through fermentation and respiration and reductive power via the pentose phosphate route.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-22T12:26:58Z
2024-10-22T12:26:58Z
2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/19816
https://onlinelibrary.wiley.com/doi/10.1111/pce.15210
Novello, M.A., Bustamante, C.A., Svetaz, L.A., Goldy, C., Valentini, G.H., Drincovich, M.F., Brotman, Y., Fernie, A.R. and Lara, M.V. (2024), Integrated Metabolomic, Lipidomic and Proteomic Analysis Define the Metabolic Changes Occurring in Curled Areas in Leaves With Leaf Peach Curl Disease. Plant, Cell & Environment. https://doi.org/10.1111/pce.15210
1365-3040
0140-7791
https://doi.org/10.1111/pce.15210
url http://hdl.handle.net/20.500.12123/19816
https://onlinelibrary.wiley.com/doi/10.1111/pce.15210
https://doi.org/10.1111/pce.15210
identifier_str_mv Novello, M.A., Bustamante, C.A., Svetaz, L.A., Goldy, C., Valentini, G.H., Drincovich, M.F., Brotman, Y., Fernie, A.R. and Lara, M.V. (2024), Integrated Metabolomic, Lipidomic and Proteomic Analysis Define the Metabolic Changes Occurring in Curled Areas in Leaves With Leaf Peach Curl Disease. Plant, Cell & Environment. https://doi.org/10.1111/pce.15210
1365-3040
0140-7791
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I125-001, Mejoramiento genético, caracterización y uso de variabilidad con aplicación de herramientas biotecnológicas en cultivos frutales
info:eu-repograntAgreement/INTA/2023-PE-L01-I105, Generación de conocimientos, tecnologías e innovaciones para una fruticultura sostenible adaptadas al riesgo ambiental y a la mecanización
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant, Cell & Environment : e15210 (October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341425639849984
score 12.623145

Publicaciones similares