The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection

Autores
Rabinovitz, Bettina Carol; Larzabal, Mariano; Vilte, Daniel Alejandro; Cataldi, Angel Adrian; Mercado, Elsa Cristina
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.
Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fuente
Vaccine 34 (25) : 2793-2797. (May 2016)
Materia
Escherichia coli
Vaccination
Mice
Infection
Vacunación
Ratón
Infección
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1001
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1001
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network_name_str INTA Digital (INTA)
spelling The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infectionRabinovitz, Bettina CarolLarzabal, MarianoVilte, Daniel AlejandroCataldi, Angel AdrianMercado, Elsa CristinaEscherichia coliVaccinationMiceInfectionVacunaciónRatónInfecciónEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina2017-08-22T13:22:37Z2017-08-22T13:22:37Z2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1001http://linkinghub.elsevier.com/retrieve/pii/S0264410X163021090264-410Xhttps://doi.org/10.1016/j.vaccine.2016.04.056Vaccine 34 (25) : 2793-2797. (May 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:39:18Zoai:localhost:20.500.12123/1001instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:39:18.993INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
title The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
spellingShingle The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
Rabinovitz, Bettina Carol
Escherichia coli
Vaccination
Mice
Infection
Vacunación
Ratón
Infección
title_short The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
title_full The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
title_fullStr The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
title_full_unstemmed The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
title_sort The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
dc.creator.none.fl_str_mv Rabinovitz, Bettina Carol
Larzabal, Mariano
Vilte, Daniel Alejandro
Cataldi, Angel Adrian
Mercado, Elsa Cristina
author Rabinovitz, Bettina Carol
author_facet Rabinovitz, Bettina Carol
Larzabal, Mariano
Vilte, Daniel Alejandro
Cataldi, Angel Adrian
Mercado, Elsa Cristina
author_role author
author2 Larzabal, Mariano
Vilte, Daniel Alejandro
Cataldi, Angel Adrian
Mercado, Elsa Cristina
author2_role author
author
author
author
dc.subject.none.fl_str_mv Escherichia coli
Vaccination
Mice
Infection
Vacunación
Ratón
Infección
topic Escherichia coli
Vaccination
Mice
Infection
Vacunación
Ratón
Infección
dc.description.none.fl_txt_mv Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.
Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
description Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
2017-08-22T13:22:37Z
2017-08-22T13:22:37Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1001
http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109
0264-410X
https://doi.org/10.1016/j.vaccine.2016.04.056
url http://hdl.handle.net/20.500.12123/1001
http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109
https://doi.org/10.1016/j.vaccine.2016.04.056
identifier_str_mv 0264-410X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Vaccine 34 (25) : 2793-2797. (May 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.976206

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