The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection
- Autores
- Rabinovitz, Bettina Carol; Larzabal, Mariano; Vilte, Daniel Alejandro; Cataldi, Angel Adrian; Mercado, Elsa Cristina
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.
Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina - Fuente
- Vaccine 34 (25) : 2793-2797. (May 2016)
- Materia
-
Escherichia coli
Vaccination
Mice
Infection
Vacunación
Ratón
Infección - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1001
Ver los metadatos del registro completo
id |
INTADig_eed196fd8e571636ca1ea7012e0cebfe |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/1001 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infectionRabinovitz, Bettina CarolLarzabal, MarianoVilte, Daniel AlejandroCataldi, Angel AdrianMercado, Elsa CristinaEscherichia coliVaccinationMiceInfectionVacunaciónRatónInfecciónEnterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings.Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina2017-08-22T13:22:37Z2017-08-22T13:22:37Z2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1001http://linkinghub.elsevier.com/retrieve/pii/S0264410X163021090264-410Xhttps://doi.org/10.1016/j.vaccine.2016.04.056Vaccine 34 (25) : 2793-2797. (May 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:28:59Zoai:localhost:20.500.12123/1001instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:28:59.464INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
title |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
spellingShingle |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection Rabinovitz, Bettina Carol Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección |
title_short |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
title_full |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
title_fullStr |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
title_full_unstemmed |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
title_sort |
The intranasal vaccination of pregnant dams with Intimin and EspBconfers protection in neonatal mice from Escherichia coli (EHEC)O157:H7 infection |
dc.creator.none.fl_str_mv |
Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina |
author |
Rabinovitz, Bettina Carol |
author_facet |
Rabinovitz, Bettina Carol Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina |
author_role |
author |
author2 |
Larzabal, Mariano Vilte, Daniel Alejandro Cataldi, Angel Adrian Mercado, Elsa Cristina |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección |
topic |
Escherichia coli Vaccination Mice Infection Vacunación Ratón Infección |
dc.description.none.fl_txt_mv |
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina |
description |
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of γ-intimin (γ-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion of the inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both γ-Int C280 and EspB. Passive immunization of newborn mice resulted in a significant increase in serum IgG titers against γ-Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with γ-Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-05 2017-08-22T13:22:37Z 2017-08-22T13:22:37Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1001 http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 0264-410X https://doi.org/10.1016/j.vaccine.2016.04.056 |
url |
http://hdl.handle.net/20.500.12123/1001 http://linkinghub.elsevier.com/retrieve/pii/S0264410X16302109 https://doi.org/10.1016/j.vaccine.2016.04.056 |
identifier_str_mv |
0264-410X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Vaccine 34 (25) : 2793-2797. (May 2016) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1846143494504054784 |
score |
12.712165 |