Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant

Autores
Cataldi, Ángel Adrián; Yevsa, Tetyana; Vilte, Daniel Alejandro; Schulze, Kai; Castro-Parodi, Mauricio Omar; Larzabal, Mariano; Ibarra, Cristina Adriana; Mercado, Elsa Cristina; Guzman, Carlos A.
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mucosal vaccine formulations based on purified recombinant C280 γ-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. After intranasal vaccination, strong humoral and cellular immune responses were observed against C280 γ-Intimin and EspB. Sera of immunized mice inhibit bacterial haemolytic activity in vitro. Antigen-specific T-cell proliferation, IL-4, IL-2 and IFN-γ producing cells, and secretory IgA were mostly detected in animals receiving MALP-2 as adjuvant. These results suggest that C280 γ-Intimin and EspB are good candidate antigens to be incorporated into mucosal vaccines against this important pathogen.
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Yevsa, Tetyana. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Schulze, Kai. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
Fil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Guzman, Carlos A.. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
Materia
EHEC
ESPB
INTIMIN
MALP-2
MUCOSAL VACCINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/160365
Acceder
id CONICETDig_bfcb5fa0a2b9ed8f7e000fea6fcb9942
oai_identifier_str oai:ri.conicet.gov.ar:11336/160365
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvantCataldi, Ángel AdriánYevsa, TetyanaVilte, Daniel AlejandroSchulze, KaiCastro-Parodi, Mauricio OmarLarzabal, MarianoIbarra, Cristina AdrianaMercado, Elsa CristinaGuzman, Carlos A.EHECESPBINTIMINMALP-2MUCOSAL VACCINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mucosal vaccine formulations based on purified recombinant C280 γ-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. After intranasal vaccination, strong humoral and cellular immune responses were observed against C280 γ-Intimin and EspB. Sera of immunized mice inhibit bacterial haemolytic activity in vitro. Antigen-specific T-cell proliferation, IL-4, IL-2 and IFN-γ producing cells, and secretory IgA were mostly detected in animals receiving MALP-2 as adjuvant. These results suggest that C280 γ-Intimin and EspB are good candidate antigens to be incorporated into mucosal vaccines against this important pathogen.Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Yevsa, Tetyana. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; AlemaniaFil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Schulze, Kai. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; AlemaniaFil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Guzman, Carlos A.. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; AlemaniaElsevier2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/160365Cataldi, Ángel Adrián; Yevsa, Tetyana; Vilte, Daniel Alejandro; Schulze, Kai; Castro-Parodi, Mauricio Omar; et al.; Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant; Elsevier; Vaccine; 26; 44; 10-2008; 5662-56670264-410XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X08009705info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2008.07.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:29:00Zoai:ri.conicet.gov.ar:11336/160365instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:29:01.069CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
title Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
spellingShingle Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
Cataldi, Ángel Adrián
EHEC
ESPB
INTIMIN
MALP-2
MUCOSAL VACCINATION
title_short Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
title_full Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
title_fullStr Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
title_full_unstemmed Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
title_sort Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant
dc.creator.none.fl_str_mv Cataldi, Ángel Adrián
Yevsa, Tetyana
Vilte, Daniel Alejandro
Schulze, Kai
Castro-Parodi, Mauricio Omar
Larzabal, Mariano
Ibarra, Cristina Adriana
Mercado, Elsa Cristina
Guzman, Carlos A.
author Cataldi, Ángel Adrián
author_facet Cataldi, Ángel Adrián
Yevsa, Tetyana
Vilte, Daniel Alejandro
Schulze, Kai
Castro-Parodi, Mauricio Omar
Larzabal, Mariano
Ibarra, Cristina Adriana
Mercado, Elsa Cristina
Guzman, Carlos A.
author_role author
author2 Yevsa, Tetyana
Vilte, Daniel Alejandro
Schulze, Kai
Castro-Parodi, Mauricio Omar
Larzabal, Mariano
Ibarra, Cristina Adriana
Mercado, Elsa Cristina
Guzman, Carlos A.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EHEC
ESPB
INTIMIN
MALP-2
MUCOSAL VACCINATION
topic EHEC
ESPB
INTIMIN
MALP-2
MUCOSAL VACCINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mucosal vaccine formulations based on purified recombinant C280 γ-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. After intranasal vaccination, strong humoral and cellular immune responses were observed against C280 γ-Intimin and EspB. Sera of immunized mice inhibit bacterial haemolytic activity in vitro. Antigen-specific T-cell proliferation, IL-4, IL-2 and IFN-γ producing cells, and secretory IgA were mostly detected in animals receiving MALP-2 as adjuvant. These results suggest that C280 γ-Intimin and EspB are good candidate antigens to be incorporated into mucosal vaccines against this important pathogen.
Fil: Cataldi, Ángel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Yevsa, Tetyana. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Schulze, Kai. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
Fil: Castro-Parodi, Mauricio Omar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Ibarra, Cristina Adriana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Guzman, Carlos A.. Helmholtz Centre for Infection Research. Department of Vaccinology and Applied Microbiology; Alemania
description Mucosal vaccine formulations based on purified recombinant C280 γ-Intimin and EspB (Escherichia coli secreted protein B) from enterohaemorragic E. coli co-administered with a pegylated derivative of the TLR2/6 agonist MALP-2 (macrophage-activating lipopeptide) as adjuvant were evaluated in BALB/c mice. After intranasal vaccination, strong humoral and cellular immune responses were observed against C280 γ-Intimin and EspB. Sera of immunized mice inhibit bacterial haemolytic activity in vitro. Antigen-specific T-cell proliferation, IL-4, IL-2 and IFN-γ producing cells, and secretory IgA were mostly detected in animals receiving MALP-2 as adjuvant. These results suggest that C280 γ-Intimin and EspB are good candidate antigens to be incorporated into mucosal vaccines against this important pathogen.
publishDate 2008
dc.date.none.fl_str_mv 2008-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/160365
Cataldi, Ángel Adrián; Yevsa, Tetyana; Vilte, Daniel Alejandro; Schulze, Kai; Castro-Parodi, Mauricio Omar; et al.; Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant; Elsevier; Vaccine; 26; 44; 10-2008; 5662-5667
0264-410X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/160365
identifier_str_mv Cataldi, Ángel Adrián; Yevsa, Tetyana; Vilte, Daniel Alejandro; Schulze, Kai; Castro-Parodi, Mauricio Omar; et al.; Efficient immune responses against Intimin and EspB of enterohaemorragic Escherichia coli after intranasal vaccination using the TLR2/6 agonist MALP-2 as adjuvant; Elsevier; Vaccine; 26; 44; 10-2008; 5662-5667
0264-410X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X08009705
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vaccine.2008.07.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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