Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis

Autores
Cuerda, Maria Ximena; Colombatti Olivieri, Maria Alejandra; Gravisaco, María José; Marfil, Maria Jimena; Barandiaran, Soledad; Sevilla, Iker A.; Garrido, Joseba M.; Moyano, Roberto Damian; Zumarraga, Martin Jose; Romano, Maria Isabel; Juste, Ramón A.; Santangelo, María De La Paz
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aim of study: To demonstrate the virulence of a Mycobacterium bovis local pig isolate in order to contribute to a better understanding of the pathological and immunological consequences of M. bovis infection in previous sensitized animals. Area of study: Buenos Aires, Argentina Material and methods: One group of ten pigs received two oral doses of killed M. bovis suspension and a comparative intradermal tuberculin test (CIT) (multiple sensitized) and then was infected with the M. bovis strain. Another group only received the CIT (single sensitized) and the infective dose. Humoral immune response was followed monthly, and gross pathology, histopathological and bacteriological analysis were performed at necropsy 100 days after infection. Main results: M. bovis oral infection induced lesions and allowed bacterial growth in most of the animals. Previous sensitization with killed M. bovis suspension slightly raised the intensity of the response, as the multiple sensitized group showed higher lesion scores and humoral response. Research highlights: Although the differences in lesion scores were not statistically significant, oral route infection after sensitization can modify the course of infections towards a fast development of lesions with a higher fibrotic component suggestive of increased resistance to infection in the right conditions.
Instituto de Biotecnología
Fil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gravisaco, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sevilla, Iker A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Garrido, Joseba M. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juste, Ramón A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Spanish Journal of Agricultural Research 20 (1) : e0502 (Marzo 2022)
Materia
Pathogenesis
Swine
Tuberculosis
Histopathology
Infectious Diseases
Patogénesis
Cerdo
Mycobacterium bovis
Histopatología
Enfermedades Infecciosas
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11593
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11593
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovisCuerda, Maria XimenaColombatti Olivieri, Maria AlejandraGravisaco, María JoséMarfil, Maria JimenaBarandiaran, SoledadSevilla, Iker A.Garrido, Joseba M.Moyano, Roberto DamianZumarraga, Martin JoseRomano, Maria IsabelJuste, Ramón A.Santangelo, María De La PazPathogenesisSwineTuberculosisHistopathologyInfectious DiseasesPatogénesisCerdoMycobacterium bovisHistopatologíaEnfermedades InfecciosasAim of study: To demonstrate the virulence of a Mycobacterium bovis local pig isolate in order to contribute to a better understanding of the pathological and immunological consequences of M. bovis infection in previous sensitized animals. Area of study: Buenos Aires, Argentina Material and methods: One group of ten pigs received two oral doses of killed M. bovis suspension and a comparative intradermal tuberculin test (CIT) (multiple sensitized) and then was infected with the M. bovis strain. Another group only received the CIT (single sensitized) and the infective dose. Humoral immune response was followed monthly, and gross pathology, histopathological and bacteriological analysis were performed at necropsy 100 days after infection. Main results: M. bovis oral infection induced lesions and allowed bacterial growth in most of the animals. Previous sensitization with killed M. bovis suspension slightly raised the intensity of the response, as the multiple sensitized group showed higher lesion scores and humoral response. Research highlights: Although the differences in lesion scores were not statistically significant, oral route infection after sensitization can modify the course of infections towards a fast development of lesions with a higher fibrotic component suggestive of increased resistance to infection in the right conditions.Instituto de BiotecnologíaFil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gravisaco, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Sevilla, Iker A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; EspañaFil: Garrido, Joseba M. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; EspañaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Juste, Ramón A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; EspañaFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaINIA (España)2022-04-08T10:37:45Z2022-04-08T10:37:45Z2022-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11593https://revistas.inia.es/index.php/sjar/article/view/184791695-971Xhttps://doi.org/10.5424/sjar/2022201-18479Spanish Journal of Agricultural Research 20 (1) : e0502 (Marzo 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:07Zoai:localhost:20.500.12123/11593instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:07.665INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
title Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
spellingShingle Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
Cuerda, Maria Ximena
Pathogenesis
Swine
Tuberculosis
Histopathology
Infectious Diseases
Patogénesis
Cerdo
Mycobacterium bovis
Histopatología
Enfermedades Infecciosas
title_short Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
title_full Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
title_fullStr Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
title_full_unstemmed Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
title_sort Pathogenesis of domestic pigs submitted to mycobacterial sensitizations previous to experimental infection with Mycobacterium bovis
dc.creator.none.fl_str_mv Cuerda, Maria Ximena
Colombatti Olivieri, Maria Alejandra
Gravisaco, María José
Marfil, Maria Jimena
Barandiaran, Soledad
Sevilla, Iker A.
Garrido, Joseba M.
Moyano, Roberto Damian
Zumarraga, Martin Jose
Romano, Maria Isabel
Juste, Ramón A.
Santangelo, María De La Paz
author Cuerda, Maria Ximena
author_facet Cuerda, Maria Ximena
Colombatti Olivieri, Maria Alejandra
Gravisaco, María José
Marfil, Maria Jimena
Barandiaran, Soledad
Sevilla, Iker A.
Garrido, Joseba M.
Moyano, Roberto Damian
Zumarraga, Martin Jose
Romano, Maria Isabel
Juste, Ramón A.
Santangelo, María De La Paz
author_role author
author2 Colombatti Olivieri, Maria Alejandra
Gravisaco, María José
Marfil, Maria Jimena
Barandiaran, Soledad
Sevilla, Iker A.
Garrido, Joseba M.
Moyano, Roberto Damian
Zumarraga, Martin Jose
Romano, Maria Isabel
Juste, Ramón A.
Santangelo, María De La Paz
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pathogenesis
Swine
Tuberculosis
Histopathology
Infectious Diseases
Patogénesis
Cerdo
Mycobacterium bovis
Histopatología
Enfermedades Infecciosas
topic Pathogenesis
Swine
Tuberculosis
Histopathology
Infectious Diseases
Patogénesis
Cerdo
Mycobacterium bovis
Histopatología
Enfermedades Infecciosas
dc.description.none.fl_txt_mv Aim of study: To demonstrate the virulence of a Mycobacterium bovis local pig isolate in order to contribute to a better understanding of the pathological and immunological consequences of M. bovis infection in previous sensitized animals. Area of study: Buenos Aires, Argentina Material and methods: One group of ten pigs received two oral doses of killed M. bovis suspension and a comparative intradermal tuberculin test (CIT) (multiple sensitized) and then was infected with the M. bovis strain. Another group only received the CIT (single sensitized) and the infective dose. Humoral immune response was followed monthly, and gross pathology, histopathological and bacteriological analysis were performed at necropsy 100 days after infection. Main results: M. bovis oral infection induced lesions and allowed bacterial growth in most of the animals. Previous sensitization with killed M. bovis suspension slightly raised the intensity of the response, as the multiple sensitized group showed higher lesion scores and humoral response. Research highlights: Although the differences in lesion scores were not statistically significant, oral route infection after sensitization can modify the course of infections towards a fast development of lesions with a higher fibrotic component suggestive of increased resistance to infection in the right conditions.
Instituto de Biotecnología
Fil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gravisaco, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gravisaco, María José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Marfil, Maria Jimena. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Sevilla, Iker A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Garrido, Joseba M. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Juste, Ramón A. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER). Departamento de Salud Animal; España
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aim of study: To demonstrate the virulence of a Mycobacterium bovis local pig isolate in order to contribute to a better understanding of the pathological and immunological consequences of M. bovis infection in previous sensitized animals. Area of study: Buenos Aires, Argentina Material and methods: One group of ten pigs received two oral doses of killed M. bovis suspension and a comparative intradermal tuberculin test (CIT) (multiple sensitized) and then was infected with the M. bovis strain. Another group only received the CIT (single sensitized) and the infective dose. Humoral immune response was followed monthly, and gross pathology, histopathological and bacteriological analysis were performed at necropsy 100 days after infection. Main results: M. bovis oral infection induced lesions and allowed bacterial growth in most of the animals. Previous sensitization with killed M. bovis suspension slightly raised the intensity of the response, as the multiple sensitized group showed higher lesion scores and humoral response. Research highlights: Although the differences in lesion scores were not statistically significant, oral route infection after sensitization can modify the course of infections towards a fast development of lesions with a higher fibrotic component suggestive of increased resistance to infection in the right conditions.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-08T10:37:45Z
2022-04-08T10:37:45Z
2022-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11593
https://revistas.inia.es/index.php/sjar/article/view/18479
1695-971X
https://doi.org/10.5424/sjar/2022201-18479
url http://hdl.handle.net/20.500.12123/11593
https://revistas.inia.es/index.php/sjar/article/view/18479
https://doi.org/10.5424/sjar/2022201-18479
identifier_str_mv 1695-971X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv INIA (España)
publisher.none.fl_str_mv INIA (España)
dc.source.none.fl_str_mv Spanish Journal of Agricultural Research 20 (1) : e0502 (Marzo 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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