Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice

Autores
Mansilla, Florencia Celeste; Quintana, Maria Eugenia; Langellotti, Cecilia Ana; Wilda, Maximiliano; Martinez, Andrea; Fonzo, Adriana; Moore, Prando Dadin; Cardoso, Nancy; Capozzo, Alejandra
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.
Inst.de Virología
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wilda, Maximiliano. Tecnovax S.A,; Argentina
Fil: Martinez, Andrea. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
Fil: Fonzo, Adriana. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Experimental parasitology 160 : 1-10. (January 2016)
Materia
Enfermedades de los Animales
Neospora Caninum
Inmunización
Linfocitos-t
Ratón
Animal Diseases
Immunization
T-lymphocytes
Mice
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1370
Acceder
id INTADig_eafd0d3d63918ddafa205a3431d51497
oai_identifier_str oai:localhost:20.500.12123/1370
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in miceMansilla, Florencia CelesteQuintana, Maria EugeniaLangellotti, Cecilia AnaWilda, MaximilianoMartinez, AndreaFonzo, AdrianaMoore, Prando DadinCardoso, NancyCapozzo, AlejandraEnfermedades de los AnimalesNeospora CaninumInmunizaciónLinfocitos-tRatónAnimal DiseasesImmunizationT-lymphocytesMiceProfilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.Inst.de VirologíaFil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Wilda, Maximiliano. Tecnovax S.A,; ArgentinaFil: Martinez, Andrea. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; ArgentinaFil: Fonzo, Adriana. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; ArgentinaFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina2017-10-02T12:25:42Z2017-10-02T12:25:42Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1370http://www.sciencedirect.com/science/article/pii/S00144894153005520014-4894 (Print)1090-2449 (Online)https://doi.org/10.1016/j.exppara.2015.10.008Experimental parasitology 160 : 1-10. (January 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:01Zoai:localhost:20.500.12123/1370instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:02.054INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
title Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
spellingShingle Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
Mansilla, Florencia Celeste
Enfermedades de los Animales
Neospora Caninum
Inmunización
Linfocitos-t
Ratón
Animal Diseases
Immunization
T-lymphocytes
Mice
title_short Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
title_full Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
title_fullStr Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
title_full_unstemmed Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
title_sort Immunization with Neospora caninum profilin induces limited protection and a regulatory T-cell response in mice
dc.creator.none.fl_str_mv Mansilla, Florencia Celeste
Quintana, Maria Eugenia
Langellotti, Cecilia Ana
Wilda, Maximiliano
Martinez, Andrea
Fonzo, Adriana
Moore, Prando Dadin
Cardoso, Nancy
Capozzo, Alejandra
author Mansilla, Florencia Celeste
author_facet Mansilla, Florencia Celeste
Quintana, Maria Eugenia
Langellotti, Cecilia Ana
Wilda, Maximiliano
Martinez, Andrea
Fonzo, Adriana
Moore, Prando Dadin
Cardoso, Nancy
Capozzo, Alejandra
author_role author
author2 Quintana, Maria Eugenia
Langellotti, Cecilia Ana
Wilda, Maximiliano
Martinez, Andrea
Fonzo, Adriana
Moore, Prando Dadin
Cardoso, Nancy
Capozzo, Alejandra
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Neospora Caninum
Inmunización
Linfocitos-t
Ratón
Animal Diseases
Immunization
T-lymphocytes
Mice
topic Enfermedades de los Animales
Neospora Caninum
Inmunización
Linfocitos-t
Ratón
Animal Diseases
Immunization
T-lymphocytes
Mice
dc.description.none.fl_txt_mv Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.
Inst.de Virología
Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quintana, Maria Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Wilda, Maximiliano. Tecnovax S.A,; Argentina
Fil: Martinez, Andrea. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
Fil: Fonzo, Adriana. Instituto de Ciencia y Tecnología Dr. Céesar Milstein; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Cardoso, Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description Profilins are actin-binding proteins that regulate the polymerization of actin filaments. In apicomplexan parasites, they are essential for invasion. Profilins also trigger the immune response of the host by activating TLRs on dendritic cells (DCs), inducing the production of pro-inflammatory cytokines. In this study we characterized for the first time the immune response and protection elicited by a vaccine based on Neospora caninum profilin in mice. Groups of eight BALB/c mice received either two doses of a recombinant N. caninum profilin expressed in Escherichia coli. (rNcPRO) or PBS, both formulated with an aqueous soy-based adjuvant enriched in TLR-agonists. Specific anti-profilin antibodies were detected in rNcPRO-vaccinated animals, mainly IgM and IgG3, which were consumed after infection. Splenocytes from rNcPRO-immunized animals proliferated after an in vitro stimulation with rNcPRO before and after challenge. An impairment of the cellular response was observed in NcPRO vaccinated and infected mice following an in vitro stimulation with native antigens of N. caninum, related to an increase in the percentage of CD4þCD25þFoxP3þ. Two out of five rNcPRO-vaccinated challenged mice were protected; they were negative for parasite DNA in the brain and showed no histopathological lesions, which were found in all PBS-vaccinated animals. As a whole, our results provide evidence of a regulatory response elicited by immunization with rNcPRO, and suggest a role of profilin in the modulation and/or evasion of immune responses against N. caninum.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-10-02T12:25:42Z
2017-10-02T12:25:42Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1370
http://www.sciencedirect.com/science/article/pii/S0014489415300552
0014-4894 (Print)
1090-2449 (Online)
https://doi.org/10.1016/j.exppara.2015.10.008
url http://hdl.handle.net/20.500.12123/1370
http://www.sciencedirect.com/science/article/pii/S0014489415300552
https://doi.org/10.1016/j.exppara.2015.10.008
identifier_str_mv 0014-4894 (Print)
1090-2449 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Experimental parasitology 160 : 1-10. (January 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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