Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo

Autores
Pappalardo, Juan Sebastián; Macairan, Jun-Ray; Macina, Alexia; Poulhazan, Alexandre; Quattrocchi, Valeria; Marcotte, Isabelle; Naccache, Rafik
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapies
Estación Experimental Agropecuaria Bariloche
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Produccion Animal; Argentina
Fil: Macairan, Jun-Ray. Concordia University. Department of Chemistry and Biochemistry; Canada
Fil: Macina, Alexia. Concordia University. Department of Chemistry and Biochemistry; Canada
Fil: Poulhazan, Alexandre. Universite du Quebec a Montreal. Department of Chemistry; Canada
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnologia Agropecuaria (INTA). Instituto de Virologia e Innovaciones Tecnológicas. Laboratorio de Bionanotecnología; Argentina
Fil: Marcotte, Isabelle. Universite du Quebec a Montreal. Department of Chemistry; Canada
Fil: Naccache, Rafik. Concordia University. Department of Chemistry and Biochemistry; Canada
Fuente
Physical Chemistry Chemical Physics 22 : 16595 (2020)
Materia
Nanomedicina
Nanotecnología
Nanopartículas
Nanomedicine
Nanotechnology
Nanoparticles
Carbon Nanoparticles
Nanopartículas Luminiscentes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
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oai:localhost:20.500.12123/7938
Acceder
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spelling Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivoPappalardo, Juan SebastiánMacairan, Jun-RayMacina, AlexiaPoulhazan, AlexandreQuattrocchi, ValeriaMarcotte, IsabelleNaccache, RafikNanomedicinaNanotecnologíaNanopartículasNanomedicineNanotechnologyNanoparticlesCarbon NanoparticlesNanopartículas LuminiscentesPassivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapiesEstación Experimental Agropecuaria BarilocheFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Produccion Animal; ArgentinaFil: Macairan, Jun-Ray. Concordia University. Department of Chemistry and Biochemistry; CanadaFil: Macina, Alexia. Concordia University. Department of Chemistry and Biochemistry; CanadaFil: Poulhazan, Alexandre. Universite du Quebec a Montreal. Department of Chemistry; CanadaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnologia Agropecuaria (INTA). Instituto de Virologia e Innovaciones Tecnológicas. Laboratorio de Bionanotecnología; ArgentinaFil: Marcotte, Isabelle. Universite du Quebec a Montreal. Department of Chemistry; CanadaFil: Naccache, Rafik. Concordia University. Department of Chemistry and Biochemistry; CanadaRoyal Society of Chemistry2020-09-22T12:35:08Z2020-09-22T12:35:08Z2020-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7938https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K1463-9084https://doi.org/10.1039/D0CP01938KPhysical Chemistry Chemical Physics 22 : 16595 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:48:37Zoai:localhost:20.500.12123/7938instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:38.144INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
title Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
spellingShingle Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
Pappalardo, Juan Sebastián
Nanomedicina
Nanotecnología
Nanopartículas
Nanomedicine
Nanotechnology
Nanoparticles
Carbon Nanoparticles
Nanopartículas Luminiscentes
title_short Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
title_full Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
title_fullStr Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
title_full_unstemmed Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
title_sort Effects of polydopamine-passivation on the optical properties of carbon dots and its potential use in vivo
dc.creator.none.fl_str_mv Pappalardo, Juan Sebastián
Macairan, Jun-Ray
Macina, Alexia
Poulhazan, Alexandre
Quattrocchi, Valeria
Marcotte, Isabelle
Naccache, Rafik
author Pappalardo, Juan Sebastián
author_facet Pappalardo, Juan Sebastián
Macairan, Jun-Ray
Macina, Alexia
Poulhazan, Alexandre
Quattrocchi, Valeria
Marcotte, Isabelle
Naccache, Rafik
author_role author
author2 Macairan, Jun-Ray
Macina, Alexia
Poulhazan, Alexandre
Quattrocchi, Valeria
Marcotte, Isabelle
Naccache, Rafik
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanomedicina
Nanotecnología
Nanopartículas
Nanomedicine
Nanotechnology
Nanoparticles
Carbon Nanoparticles
Nanopartículas Luminiscentes
topic Nanomedicina
Nanotecnología
Nanopartículas
Nanomedicine
Nanotechnology
Nanoparticles
Carbon Nanoparticles
Nanopartículas Luminiscentes
dc.description.none.fl_txt_mv Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapies
Estación Experimental Agropecuaria Bariloche
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Área de Produccion Animal; Argentina
Fil: Macairan, Jun-Ray. Concordia University. Department of Chemistry and Biochemistry; Canada
Fil: Macina, Alexia. Concordia University. Department of Chemistry and Biochemistry; Canada
Fil: Poulhazan, Alexandre. Universite du Quebec a Montreal. Department of Chemistry; Canada
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnologia Agropecuaria (INTA). Instituto de Virologia e Innovaciones Tecnológicas. Laboratorio de Bionanotecnología; Argentina
Fil: Marcotte, Isabelle. Universite du Quebec a Montreal. Department of Chemistry; Canada
Fil: Naccache, Rafik. Concordia University. Department of Chemistry and Biochemistry; Canada
description Passivation of carbon dots via heteroatom doping has been shown to enhance their optical properties and tune their fluorescence signature. Additionally, the incorporation of polymeric precursors in carbon dot synthesis has gained considerable interest with benefits to biological applications namely bioimaging, drug delivery and sensing, among others. In order to combine the desirable attributes of both, fluorescence enhancement and increased biocompatibility, polymers composed of high aromaticity and nitrogen content can be used as efficient carbon dot passivating agents. Here, the synthesis of fluorescent polymer-passivated carbon dots was developed through a microwave-assisted pyrolysis reaction of galactose, citric acid and polydopamine. Passivation of the dots with polydopamine induces a 90 nm redshift in the fluorescence maxima from 420 to 510 nm. Moreover, passivation results in excitationindependent fluorescence and a 3.5-fold increase in fluorescence quantum yield, which increases from 1.3 to 4.6%. The application of the carbon dots as imaging probes was investigated in in vitro and in vivo model systems. Cytotoxicity studies in J774 and CHO-K1 cell lines revealed reduced cell toxicity for the polydopamine-passivated carbon dots in comparison to their unpassivated counterpart. In BALB/c mice, biodistribution studies demonstrated that regardless of surface passivation, the dots predominantly remained in the circulatory system 90 minutes post inoculation suggesting their potential use for cardiovascular therapies
publishDate 2020
dc.date.none.fl_str_mv 2020-09-22T12:35:08Z
2020-09-22T12:35:08Z
2020-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7938
https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K
1463-9084
https://doi.org/10.1039/D0CP01938K
url http://hdl.handle.net/20.500.12123/7938
https://pubs.rsc.org/en/content/articlelanding/2020/CP/D0CP01938K
https://doi.org/10.1039/D0CP01938K
identifier_str_mv 1463-9084
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Physical Chemistry Chemical Physics 22 : 16595 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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