Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS

Autores
Dieguez, Maria Jose; Pergolesi, María Fernanda; Velásquez, Silvia Melina; Ingala, Lorena Romina; López, M.; Darino, Martin Alejandro; Paux, Etienne; Feuillet, Catherine; Sacco, Francisco
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.
Instituto de Genética
Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fuente
Theoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014)
Materia
Trigo
Enfermedades de las Plantas
Roya
Resistencia Genética
Cromosomas
Marcadores Genéticos
Wheat
Plant Diseases
Rusts
Genetic Resistance
Chromosomes
Genetic Markers
Marcadores Moleculares
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4333
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4333
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network_name_str INTA Digital (INTA)
spelling Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BSDieguez, Maria JosePergolesi, María FernandaVelásquez, Silvia MelinaIngala, Lorena RominaLópez, M.Darino, Martin AlejandroPaux, EtienneFeuillet, CatherineSacco, FranciscoTrigoEnfermedades de las PlantasRoyaResistencia GenéticaCromosomasMarcadores GenéticosWheatPlant DiseasesRustsGenetic ResistanceChromosomesGenetic MarkersMarcadores MolecularesThree leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.Instituto de GenéticaFil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; FranciaFil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; FranciaFil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2019-01-25T13:24:09Z2019-01-25T13:24:09Z2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00122-014-2285-zhttp://hdl.handle.net/20.500.12123/43330040-57521432-2242https://doi.org/10.1007/s00122-014-2285-zTheoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:16:49Zoai:localhost:20.500.12123/4333instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:49.31INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
spellingShingle Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
Dieguez, Maria Jose
Trigo
Enfermedades de las Plantas
Roya
Resistencia Genética
Cromosomas
Marcadores Genéticos
Wheat
Plant Diseases
Rusts
Genetic Resistance
Chromosomes
Genetic Markers
Marcadores Moleculares
title_short Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_full Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_fullStr Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_full_unstemmed Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
title_sort Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
dc.creator.none.fl_str_mv Dieguez, Maria Jose
Pergolesi, María Fernanda
Velásquez, Silvia Melina
Ingala, Lorena Romina
López, M.
Darino, Martin Alejandro
Paux, Etienne
Feuillet, Catherine
Sacco, Francisco
author Dieguez, Maria Jose
author_facet Dieguez, Maria Jose
Pergolesi, María Fernanda
Velásquez, Silvia Melina
Ingala, Lorena Romina
López, M.
Darino, Martin Alejandro
Paux, Etienne
Feuillet, Catherine
Sacco, Francisco
author_role author
author2 Pergolesi, María Fernanda
Velásquez, Silvia Melina
Ingala, Lorena Romina
López, M.
Darino, Martin Alejandro
Paux, Etienne
Feuillet, Catherine
Sacco, Francisco
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trigo
Enfermedades de las Plantas
Roya
Resistencia Genética
Cromosomas
Marcadores Genéticos
Wheat
Plant Diseases
Rusts
Genetic Resistance
Chromosomes
Genetic Markers
Marcadores Moleculares
topic Trigo
Enfermedades de las Plantas
Roya
Resistencia Genética
Cromosomas
Marcadores Genéticos
Wheat
Plant Diseases
Rusts
Genetic Resistance
Chromosomes
Genetic Markers
Marcadores Moleculares
dc.description.none.fl_txt_mv Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.
Instituto de Genética
Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pergolesi, Marí­a Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
description Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.
publishDate 2014
dc.date.none.fl_str_mv 2014-05
2019-01-25T13:24:09Z
2019-01-25T13:24:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00122-014-2285-z
http://hdl.handle.net/20.500.12123/4333
0040-5752
1432-2242
https://doi.org/10.1007/s00122-014-2285-z
url https://link.springer.com/article/10.1007/s00122-014-2285-z
http://hdl.handle.net/20.500.12123/4333
https://doi.org/10.1007/s00122-014-2285-z
identifier_str_mv 0040-5752
1432-2242
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Theoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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