Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS
- Autores
- Dieguez, Maria Jose; Pergolesi, María Fernanda; Velásquez, Silvia Melina; Ingala, Lorena Romina; López, M.; Darino, Martin Alejandro; Paux, Etienne; Feuillet, Catherine; Sacco, Francisco
- Año de publicación
- 2014
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.
Instituto de Genética
Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Pergolesi, María Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia
Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina - Fuente
- Theoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014)
- Materia
-
Trigo
Enfermedades de las Plantas
Roya
Resistencia Genética
Cromosomas
Marcadores Genéticos
Wheat
Plant Diseases
Rusts
Genetic Resistance
Chromosomes
Genetic Markers
Marcadores Moleculares - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4333
Ver los metadatos del registro completo
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Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BSDieguez, Maria JosePergolesi, María FernandaVelásquez, Silvia MelinaIngala, Lorena RominaLópez, M.Darino, Martin AlejandroPaux, EtienneFeuillet, CatherineSacco, FranciscoTrigoEnfermedades de las PlantasRoyaResistencia GenéticaCromosomasMarcadores GenéticosWheatPlant DiseasesRustsGenetic ResistanceChromosomesGenetic MarkersMarcadores MolecularesThree leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS.Instituto de GenéticaFil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Pergolesi, María Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; FranciaFil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; FranciaFil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaSpringer2019-01-25T13:24:09Z2019-01-25T13:24:09Z2014-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00122-014-2285-zhttp://hdl.handle.net/20.500.12123/43330040-57521432-2242https://doi.org/10.1007/s00122-014-2285-zTheoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4333instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.141INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
spellingShingle |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS Dieguez, Maria Jose Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares |
title_short |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_full |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_fullStr |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_full_unstemmed |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
title_sort |
Fine mapping of LrSV2, a race-specific adult plant leaf rust resistance gene on wheat chromosome 3BS |
dc.creator.none.fl_str_mv |
Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco |
author |
Dieguez, Maria Jose |
author_facet |
Dieguez, Maria Jose Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco |
author_role |
author |
author2 |
Pergolesi, María Fernanda Velásquez, Silvia Melina Ingala, Lorena Romina López, M. Darino, Martin Alejandro Paux, Etienne Feuillet, Catherine Sacco, Francisco |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares |
topic |
Trigo Enfermedades de las Plantas Roya Resistencia Genética Cromosomas Marcadores Genéticos Wheat Plant Diseases Rusts Genetic Resistance Chromosomes Genetic Markers Marcadores Moleculares |
dc.description.none.fl_txt_mv |
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. Instituto de Genética Fil: Dieguez, María José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Pergolesi, María Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Velásquez, Silvia Melina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Ingala, Lorena Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: López, M. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Darino, Martín Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Paux, Etienne. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia Fil: Feuillet, Catherine. Institut National de la Recherche Agronomique. UMR Genetics, Diversity and Ecophysiology of Cereals; Francia Fil: Sacco, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina |
description |
Three leaf rust resistance genes were identified in the durable leaf rust resistant Argentinean wheat variety Sinvalocho MA: the seedling resistance gene Lr3 on distal 6BL and two adult plant resistance genes, LrSV1 and LrSV2, on chromosomes 2DS and 3BS, respectively. To develop a high-resolution genetic map for LrSV2, 10 markers were genotyped on 343 F2 individuals from a cross between Sinvalocho MA and Gama6. The closest co-dominant markers on both sides of the gene (3 microsatellites and 2 STMs) were analyzed on 965 additional F2s from the same cross. Microsatellite marker cfb5010 cosegregated with LrSV2 whereas flanking markers were found at 1 cM distal and at 0.3 cM proximal to the gene. SSR markers designed from the sequences of cv Chinese Spring BAC clones spanning the LrSV2 genetic interval were tested on the recombinants, allowing the identification of microsatellite swm13 at 0.15 cM distal to LrSV2. This delimited an interval of 0.45 cM around the gene flanked by the SSR markers swm13 and gwm533 at the subtelomeric end of chromosome 3BS. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05 2019-01-25T13:24:09Z 2019-01-25T13:24:09Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://link.springer.com/article/10.1007/s00122-014-2285-z http://hdl.handle.net/20.500.12123/4333 0040-5752 1432-2242 https://doi.org/10.1007/s00122-014-2285-z |
url |
https://link.springer.com/article/10.1007/s00122-014-2285-z http://hdl.handle.net/20.500.12123/4333 https://doi.org/10.1007/s00122-014-2285-z |
identifier_str_mv |
0040-5752 1432-2242 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Theoretical and Applied Genetics 127 (5) : 1133–1141 (May 2014) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619130174439424 |
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12.559606 |