Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)

Autores
Debat, Humberto Julio; Fei Fan Ng, Terry
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fei Fan Ng, Terry. University of Georgia. College of Veterinary Medicine; Estados Unidos
Fuente
Archives of virology 164 (6) : 1727–1732. (June 2019)
Materia
Hepatitis
Virus de los Animales
Rana
Genomas
Secuencia Nucleotídica
Animal Viruses
Frogs
Genomes
Nucleotide Sequence
Hepatitis B
Rana Tibetana
Odorrana Tormota
Tibetan Frog
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)Debat, Humberto JulioFei Fan Ng, TerryHepatitisVirus de los AnimalesRanaGenomasSecuencia NucleotídicaAnimal VirusesFrogsGenomesNucleotide SequenceHepatitis BRana TibetanaOdorrana TormotaTibetan FrogViruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains.Instituto de Patología VegetalFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Fei Fan Ng, Terry. University of Georgia. College of Veterinary Medicine; Estados UnidosSpringer2019-07-04T14:55:45Z2019-07-04T14:55:45Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/5439https://link.springer.com/article/10.1007/s00705-019-04227-80304-86081432-8798 (Online)https://doi.org/10.1007/s00705-019-04227-8Archives of virology 164 (6) : 1727–1732. (June 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:42Zoai:localhost:20.500.12123/5439instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:42.792INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
title Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
spellingShingle Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
Debat, Humberto Julio
Hepatitis
Virus de los Animales
Rana
Genomas
Secuencia Nucleotídica
Animal Viruses
Frogs
Genomes
Nucleotide Sequence
Hepatitis B
Rana Tibetana
Odorrana Tormota
Tibetan Frog
title_short Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
title_full Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
title_fullStr Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
title_full_unstemmed Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
title_sort Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave‑eared torrent frog (Odorrana tormota)
dc.creator.none.fl_str_mv Debat, Humberto Julio
Fei Fan Ng, Terry
author Debat, Humberto Julio
author_facet Debat, Humberto Julio
Fei Fan Ng, Terry
author_role author
author2 Fei Fan Ng, Terry
author2_role author
dc.subject.none.fl_str_mv Hepatitis
Virus de los Animales
Rana
Genomas
Secuencia Nucleotídica
Animal Viruses
Frogs
Genomes
Nucleotide Sequence
Hepatitis B
Rana Tibetana
Odorrana Tormota
Tibetan Frog
topic Hepatitis
Virus de los Animales
Rana
Genomas
Secuencia Nucleotídica
Animal Viruses
Frogs
Genomes
Nucleotide Sequence
Hepatitis B
Rana Tibetana
Odorrana Tormota
Tibetan Frog
dc.description.none.fl_txt_mv Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains.
Instituto de Patología Vegetal
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Fei Fan Ng, Terry. University of Georgia. College of Veterinary Medicine; Estados Unidos
description Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (genus Avihepadnavirus). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog (Odorrana tormota, family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains.
publishDate 2019
dc.date.none.fl_str_mv 2019-07-04T14:55:45Z
2019-07-04T14:55:45Z
2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/5439
https://link.springer.com/article/10.1007/s00705-019-04227-8
0304-8608
1432-8798 (Online)
https://doi.org/10.1007/s00705-019-04227-8
url http://hdl.handle.net/20.500.12123/5439
https://link.springer.com/article/10.1007/s00705-019-04227-8
https://doi.org/10.1007/s00705-019-04227-8
identifier_str_mv 0304-8608
1432-8798 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Archives of virology 164 (6) : 1727–1732. (June 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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