In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection

Autores
Delfosse, Veronica Cecilia; Tasat, Deborah Ruth; Gioffre, Andrea
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria (EM) are opportunist pathogens that can affect a variety of immune compromised patients, which impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control), (b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected. Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear (PMN) cells influx and a rise in O2 2 generation. Exposure to ROFA before M. phlei infection reduced total cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2 2 generation, TNFa (p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute short-term pre-exposure to ROFA reduces early lung response to EM infection
Instituto de Biotecnología
Fil: Delfosse, Verónica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Gioffre, Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Environmental toxicology 30 (5) : 589-596. (May 2015)
Materia
Mycobacterium
Respuesta Inmunológica
Immune Response
Pulmones
Lungs
Ceniza
Ashes
In Vivo Experimentation
Mycobacterium Phlei
ROFA
Residual Oil Fly Ash
Environmental Mycobacteria
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4652
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spelling In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infectionDelfosse, Veronica CeciliaTasat, Deborah RuthGioffre, AndreaMycobacteriumRespuesta InmunológicaImmune ResponsePulmonesLungsCenizaAshesIn Vivo ExperimentationMycobacterium PhleiROFAResidual Oil Fly AshEnvironmental MycobacteriaEpidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria (EM) are opportunist pathogens that can affect a variety of immune compromised patients, which impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control), (b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected. Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear (PMN) cells influx and a rise in O2 2 generation. Exposure to ROFA before M. phlei infection reduced total cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2 2 generation, TNFa (p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute short-term pre-exposure to ROFA reduces early lung response to EM infectionInstituto de BiotecnologíaFil: Delfosse, Verónica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; ArgentinaFil: Gioffre, Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaWiley2019-03-19T13:03:56Z2019-03-19T13:03:56Z2015-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/46521520-4081https://doi.org/10.1002/tox.21936Environmental toxicology 30 (5) : 589-596. (May 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:28Zoai:localhost:20.500.12123/4652instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:28.784INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
title In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
spellingShingle In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
Delfosse, Veronica Cecilia
Mycobacterium
Respuesta Inmunológica
Immune Response
Pulmones
Lungs
Ceniza
Ashes
In Vivo Experimentation
Mycobacterium Phlei
ROFA
Residual Oil Fly Ash
Environmental Mycobacteria
title_short In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
title_full In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
title_fullStr In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
title_full_unstemmed In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
title_sort In vivo short-term exposure to residual oil fly ash impairs pulmonary innate immune response against environmental mycobacterium infection
dc.creator.none.fl_str_mv Delfosse, Veronica Cecilia
Tasat, Deborah Ruth
Gioffre, Andrea
author Delfosse, Veronica Cecilia
author_facet Delfosse, Veronica Cecilia
Tasat, Deborah Ruth
Gioffre, Andrea
author_role author
author2 Tasat, Deborah Ruth
Gioffre, Andrea
author2_role author
author
dc.subject.none.fl_str_mv Mycobacterium
Respuesta Inmunológica
Immune Response
Pulmones
Lungs
Ceniza
Ashes
In Vivo Experimentation
Mycobacterium Phlei
ROFA
Residual Oil Fly Ash
Environmental Mycobacteria
topic Mycobacterium
Respuesta Inmunológica
Immune Response
Pulmones
Lungs
Ceniza
Ashes
In Vivo Experimentation
Mycobacterium Phlei
ROFA
Residual Oil Fly Ash
Environmental Mycobacteria
dc.description.none.fl_txt_mv Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria (EM) are opportunist pathogens that can affect a variety of immune compromised patients, which impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control), (b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected. Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear (PMN) cells influx and a rise in O2 2 generation. Exposure to ROFA before M. phlei infection reduced total cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2 2 generation, TNFa (p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute short-term pre-exposure to ROFA reduces early lung response to EM infection
Instituto de Biotecnología
Fil: Delfosse, Verónica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Tasat, Deborah Ruth. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Universidad de Buenos Aires. Facultad de Odontología. Cátedra de Histología y Embriología; Argentina
Fil: Gioffre, Andrea. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Epidemiological studies have shown that pollution derived from industrial and vehicular transportation induces adverse health effects causing broad ambient respiratory diseases. Therefore, air pollution should be taken into account when microbial diseases are evaluated. Environmental mycobacteria (EM) are opportunist pathogens that can affect a variety of immune compromised patients, which impacts significantly on human morbidity and mortality. The aim of this study was to evaluate the effect of residual oil fly ash (ROFA) pre-exposure on the pulmonary response after challenge with opportunistic mycobacteria by means of an acute short-term in vivo experimental animal model. We exposed BALB/c mice to ROFA and observed a significant reduction on bacterial clearance at 24 h post infection. To study the basis of this impaired response four groups of animals were instilled with (a) saline solution (Control), (b) ROFA (1 mg kg21 BW), (c) ROFA and EM-infected (Mycobacterium phlei, 8 3 106 CFU), and (d) EMinfected. Animals were sacrificed 24 h postinfection and biomarkers of lung injury and proinflammatory madiators were examined in the bronchoalveolar lavage. Our results indicate that ROFA was able to produce an acute pulmonary injury characterized by an increase in bronchoalveolar polymorphonuclear (PMN) cells influx and a rise in O2 2 generation. Exposure to ROFA before M. phlei infection reduced total cell number and caused a significant decline in PMN cells recruitment (p<0.05), O2 2 generation, TNFa (p<0.001), and IL-6 (p<0.001) levels. Hence, our results suggest that, in this animal model, the acute short-term pre-exposure to ROFA reduces early lung response to EM infection
publishDate 2015
dc.date.none.fl_str_mv 2015-05
2019-03-19T13:03:56Z
2019-03-19T13:03:56Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4652
1520-4081
https://doi.org/10.1002/tox.21936
url http://hdl.handle.net/20.500.12123/4652
https://doi.org/10.1002/tox.21936
identifier_str_mv 1520-4081
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Environmental toxicology 30 (5) : 589-596. (May 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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