Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice

Autores
Blanco, Federico Carlos; Klepp, Laura Ines; Vazquez, Cristina Lourdes; Bigi, Fabiana
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment. Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test. Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing. Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials.
Instituto de Biotecnología
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vazquez, Cristina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
International Journal of Mycobacteriology 14 (2) : 140-144 (Apr-Jun 2025)
Materia
Mycobacterium bovis
Vaccines
In vitro
Lungs
Mice
Vacuna
Pulmones
Ratón
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23108
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23108
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected miceBlanco, Federico CarlosKlepp, Laura InesVazquez, Cristina LourdesBigi, FabianaMycobacterium bovisVaccinesIn vitroLungsMiceVacunaPulmonesRatónBackground: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment. Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test. Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing. Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials.Instituto de BiotecnologíaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Vazquez, Cristina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWolters Kluwer Medknow2025-07-21T14:57:55Z2025-07-21T14:57:55Z2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23108https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx2212-5531https://doi.org/10.4103/ijmy.ijmy_32_25International Journal of Mycobacteriology 14 (2) : 140-144 (Apr-Jun 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:38Zoai:localhost:20.500.12123/23108instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:38.86INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
title Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
spellingShingle Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
Blanco, Federico Carlos
Mycobacterium bovis
Vaccines
In vitro
Lungs
Mice
Vacuna
Pulmones
Ratón
title_short Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
title_full Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
title_fullStr Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
title_full_unstemmed Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
title_sort Dynamics of the in vitro growing of Mycobacterium bovis from the lungs of vaccinated and infected mice
dc.creator.none.fl_str_mv Blanco, Federico Carlos
Klepp, Laura Ines
Vazquez, Cristina Lourdes
Bigi, Fabiana
author Blanco, Federico Carlos
author_facet Blanco, Federico Carlos
Klepp, Laura Ines
Vazquez, Cristina Lourdes
Bigi, Fabiana
author_role author
author2 Klepp, Laura Ines
Vazquez, Cristina Lourdes
Bigi, Fabiana
author2_role author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
Vaccines
In vitro
Lungs
Mice
Vacuna
Pulmones
Ratón
topic Mycobacterium bovis
Vaccines
In vitro
Lungs
Mice
Vacuna
Pulmones
Ratón
dc.description.none.fl_txt_mv Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment. Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test. Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing. Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials.
Instituto de Biotecnología
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Vazquez, Cristina Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: Bovine tuberculosis (bTB) is a disease primarily caused by Mycobacterium bovis. Currently, no commercial vaccines exist for controlling bTB, making the development of effective vaccine candidates and testing models a high priority. Mouse models are widely used in preclinical trials of anti-TB vaccines. Determining the appropriate cultivation time to assess the mycobacterial load in animal organs or biological samples is crucial to establishing a reliable model that can accurately evaluate the effectiveness of a vaccine candidate. The aim of this study was to assess the growth dynamics and the appearance of colony-forming units (CFUs) in lung homogenates from mice infected with M. bovis. We compared the CFU counts from vaccinated and challenged mice with M. bovis using data from a previous experiment. Methods: CFUs obtained from the lungs of vaccinated and M. bovis-challenged mice of a previous experiment were registered at 3 and 4 weeks of culturing in solid media. The statistical analysis was performed with Kruskal–Wallis, followed by a Dunn’s multiple comparison test. Results: On analyzing the CFU dynamics from lung homogenates, we found that mice vaccinated with Bacillus Calmette–Guérin preserved stable CFU counts after 3 weeks of cultivation on a solid medium. In contrast, both the unvaccinated group and the group vaccinated with an attenuated M. bovis triple mutant strain reached their final CFU counts only after 4 weeks of culturing. Conclusion: These findings underscore the importance of prolonged follow-up to accurately assess CFU counts, which are crucial for determining vaccine efficacy in trials.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-21T14:57:55Z
2025-07-21T14:57:55Z
2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23108
https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx
2212-5531
https://doi.org/10.4103/ijmy.ijmy_32_25
url http://hdl.handle.net/20.500.12123/23108
https://journals.lww.com/ijmy/fulltext/2025/04000/dynamics_of_the_in_vitro_growing_of_mycobacterium.8.aspx
https://doi.org/10.4103/ijmy.ijmy_32_25
identifier_str_mv 2212-5531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wolters Kluwer Medknow
publisher.none.fl_str_mv Wolters Kluwer Medknow
dc.source.none.fl_str_mv International Journal of Mycobacteriology 14 (2) : 140-144 (Apr-Jun 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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