Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa

Autores
Luño, Victoria; Gil, María Lydia; Olaciregui, Maite; Jerez, Rodrigo A.; De Blas, José Ignacio; Hozbor, Federico Andres
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.
EEA Balcarce
Fil: Luño, Victoria. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Gil, María Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Olaciregui, Maite. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Jerez, Rodrigo A. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: de Blas, Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fuente
Andrologia 47 (9) : 1004-1011 (November 2015)
Materia
Ilex paraguariensis
Mate
Elissa officinalis
Antioxidantes
ADN
Espermatozoo
Criopreservación
Verraco
Cerdo
Antioxidants
DNA
Spermatozoa
Cryopreservation
Boars
Swine
Melissa officinalis
Esperma
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4737
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4737
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network_name_str INTA Digital (INTA)
spelling Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoaLuño, VictoriaGil, María LydiaOlaciregui, MaiteJerez, Rodrigo A.De Blas, José IgnacioHozbor, Federico AndresIlex paraguariensisMateElissa officinalisAntioxidantesADNEspermatozooCriopreservaciónVerracoCerdoAntioxidantsDNASpermatozoaCryopreservationBoarsSwineMelissa officinalisEspermaIn this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.EEA BalcarceFil: Luño, Victoria. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; EspañaFil: Gil, María Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; EspañaFil: Olaciregui, Maite. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; EspañaFil: Jerez, Rodrigo A. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; EspañaFil: de Blas, Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; EspañaFil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaWiley2019-03-26T12:11:57Z2019-03-26T12:11:57Z2015-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370http://hdl.handle.net/20.500.12123/47370303-45691439-0272https://doi.org/10.1111/and.12370Andrologia 47 (9) : 1004-1011 (November 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:29Zoai:localhost:20.500.12123/4737instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:29.495INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
title Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
spellingShingle Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
Luño, Victoria
Ilex paraguariensis
Mate
Elissa officinalis
Antioxidantes
ADN
Espermatozoo
Criopreservación
Verraco
Cerdo
Antioxidants
DNA
Spermatozoa
Cryopreservation
Boars
Swine
Melissa officinalis
Esperma
title_short Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
title_full Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
title_fullStr Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
title_full_unstemmed Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
title_sort Antioxidant effect of lemon balm (Melissa officinalis) and mate tea (Ilex paraguensys) on quality, lipid peroxidation and DNA oxidation of cryopreserved boar epididymal spermatozoa
dc.creator.none.fl_str_mv Luño, Victoria
Gil, María Lydia
Olaciregui, Maite
Jerez, Rodrigo A.
De Blas, José Ignacio
Hozbor, Federico Andres
author Luño, Victoria
author_facet Luño, Victoria
Gil, María Lydia
Olaciregui, Maite
Jerez, Rodrigo A.
De Blas, José Ignacio
Hozbor, Federico Andres
author_role author
author2 Gil, María Lydia
Olaciregui, Maite
Jerez, Rodrigo A.
De Blas, José Ignacio
Hozbor, Federico Andres
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ilex paraguariensis
Mate
Elissa officinalis
Antioxidantes
ADN
Espermatozoo
Criopreservación
Verraco
Cerdo
Antioxidants
DNA
Spermatozoa
Cryopreservation
Boars
Swine
Melissa officinalis
Esperma
topic Ilex paraguariensis
Mate
Elissa officinalis
Antioxidantes
ADN
Espermatozoo
Criopreservación
Verraco
Cerdo
Antioxidants
DNA
Spermatozoa
Cryopreservation
Boars
Swine
Melissa officinalis
Esperma
dc.description.none.fl_txt_mv In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.
EEA Balcarce
Fil: Luño, Victoria. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Gil, María Lydia. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Olaciregui, Maite. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Jerez, Rodrigo A. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: de Blas, Ignacio. Universidad de Zaragoza. Facultad de Veterinaria. Departamento de Patología Animal; España
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
description In this study, we investigated the protective ability of the addition of two antioxidant herb extracts, mate tea and lemon balm, on boar epididymal frozen–thawed spermatozoa quality. Testes from mature boars were collected at local slaughterhouse, and sperm samples from epididymis were recovered by flushing. Spermatozoa were cryopreserved in lactose–egg yolk buffer supplemented with various concentrations of lemon balm and mate tea (0, 2.5, 5 and 10 g l−1) using the straw‐freezing procedure. Motion parameters, acrosome and plasma membrane integrity, lipoperoxidation levels and DNA oxidative damage (8‐hydroxy‐2′‐deoxyguanosine base lesion) were evaluated. There were no differences among experimental groups with regard to motility characteristics, viability, acrosome and plasma membrane integrity; however, the highest concentration of lemon balm produced significant (P < 0.05) improvement in curvilinear trajectory, straightness and amplitude of lateral head displacement after thawing. The supplementation of freezing extender with mate tea and lemon balm reduced sperm lipid membrane peroxidation, and only mate tea protected DNA against oxidative damage during cryopreservation at 120 min post‐thawing (P < 0.05). Mate tea experimental extender at concentration of 10 g l−1 showed the lowest percentage of sperm oxidised DNA and malondialdehyde generation; thus, mate tea is a potential candidate such as antioxidant compound on boar sperm cryopreservation medium.
publishDate 2015
dc.date.none.fl_str_mv 2015-11
2019-03-26T12:11:57Z
2019-03-26T12:11:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370
http://hdl.handle.net/20.500.12123/4737
0303-4569
1439-0272
https://doi.org/10.1111/and.12370
url https://onlinelibrary.wiley.com/doi/abs/10.1111/and.12370
http://hdl.handle.net/20.500.12123/4737
https://doi.org/10.1111/and.12370
identifier_str_mv 0303-4569
1439-0272
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Andrologia 47 (9) : 1004-1011 (November 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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