Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid

Autores
Luongo, Chiara; Llamas-López, Pedro José; Garrappa, Gabriela; Rodríguez-Tobón, Ernesto; Grudzinska, Paulina; García Vázquez, Francisco Alberto
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Luongo, Chiara. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Llamas-López, Pedro José. Universidad Miguel Hernández. Departamento de Tecnología Agroalimentaria; España
Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina
Fil: Rodríguez-Tobón, Ernesto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Rodríguez-Tobón, Ernesto. Universidad Autónoma Metropolitana. Unidad Iztapalapa. Departamento de Biología de La Reproducción; México
Fil: Grudzinska, Paulina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España
Fuente
Scientific Reports 13 : Article number: 15258 (2023)
Materia
Verraco
Cerdo
Espermatozoo
Reproducción Animal
Calidad
Boars
Swine
Spermatozoa
Semen
Animal Reproduction
Quality
Esperma
Plasma Seminal
Sperm
Seminal Plasma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23941
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23941
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluidLuongo, ChiaraLlamas-López, Pedro JoséGarrappa, GabrielaRodríguez-Tobón, ErnestoGrudzinska, PaulinaGarcía Vázquez, Francisco AlbertoVerracoCerdoEspermatozooReproducción AnimalCalidadBoarsSwineSpermatozoaSemenAnimal ReproductionQualityEspermaPlasma SeminalSpermSeminal PlasmaBoar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions.Instituto de Investigación Animal del Chaco SemiáridoFil: Luongo, Chiara. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: Llamas-López, Pedro José. Universidad Miguel Hernández. Departamento de Tecnología Agroalimentaria; EspañaFil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Rodríguez-Tobón, Ernesto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: Rodríguez-Tobón, Ernesto. Universidad Autónoma Metropolitana. Unidad Iztapalapa. Departamento de Biología de La Reproducción; MéxicoFil: Grudzinska, Paulina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; EspañaFil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); EspañaSpringer Nature2025-09-25T12:21:40Z2025-09-25T12:21:40Z2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23941https://www.nature.com/articles/s41598-023-42254-32045-2322https://doi.org/10.1038/s41598-023-42254-3Scientific Reports 13 : Article number: 15258 (2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:32Zoai:localhost:20.500.12123/23941instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:33.23INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
title Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
spellingShingle Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
Luongo, Chiara
Verraco
Cerdo
Espermatozoo
Reproducción Animal
Calidad
Boars
Swine
Spermatozoa
Semen
Animal Reproduction
Quality
Esperma
Plasma Seminal
Sperm
Seminal Plasma
title_short Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
title_full Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
title_fullStr Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
title_full_unstemmed Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
title_sort Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid
dc.creator.none.fl_str_mv Luongo, Chiara
Llamas-López, Pedro José
Garrappa, Gabriela
Rodríguez-Tobón, Ernesto
Grudzinska, Paulina
García Vázquez, Francisco Alberto
author Luongo, Chiara
author_facet Luongo, Chiara
Llamas-López, Pedro José
Garrappa, Gabriela
Rodríguez-Tobón, Ernesto
Grudzinska, Paulina
García Vázquez, Francisco Alberto
author_role author
author2 Llamas-López, Pedro José
Garrappa, Gabriela
Rodríguez-Tobón, Ernesto
Grudzinska, Paulina
García Vázquez, Francisco Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Verraco
Cerdo
Espermatozoo
Reproducción Animal
Calidad
Boars
Swine
Spermatozoa
Semen
Animal Reproduction
Quality
Esperma
Plasma Seminal
Sperm
Seminal Plasma
topic Verraco
Cerdo
Espermatozoo
Reproducción Animal
Calidad
Boars
Swine
Spermatozoa
Semen
Animal Reproduction
Quality
Esperma
Plasma Seminal
Sperm
Seminal Plasma
dc.description.none.fl_txt_mv Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Luongo, Chiara. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Llamas-López, Pedro José. Universidad Miguel Hernández. Departamento de Tecnología Agroalimentaria; España
Fil: Garrappa, Gabriela. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Garrappa, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina
Fil: Rodríguez-Tobón, Ernesto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: Rodríguez-Tobón, Ernesto. Universidad Autónoma Metropolitana. Unidad Iztapalapa. Departamento de Biología de La Reproducción; México
Fil: Grudzinska, Paulina. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Universidad de Murcia. Facultad de Veterinaria. Campus de Excelencia Mare Nostrum. Departamento de Fisiología; España
Fil: García Vázquez, Francisco Alberto. Instituto Murciano de Investigación Biosanitaria (IMIB); España
description Boar ejaculate is composed of sperm cells and seminal plasma (SP) and is emitted in different fractions (pre-sperm fraction; spermatic-rich fraction; intermediate fraction; post-spermatic fraction), with different composition of SP and volume, which could influence the sperm quality during seminal doses preparation, conservation, and interaction with the female reproductive tract. In artificial insemination (AI) centers, seminal doses are usually prepared with the spermatic-rich and intermediate fractions, but the inclusion of other ejaculate fractions, although controversial, is beginning to be applied. The objective was to evaluate the synergic effect of accumulative ejaculated fractions on sperm functionality during seminal doses preparation, throughout storage and after incubation with uterine fluid (UF). For this purpose, a total of 57 ejaculates were collected, and the following experimental groups were prepared (n = 19 per group): (F1) spermatic-rich fraction; (F2) F1 plus intermediate fraction; (F3) F2 plus post-spermatic fraction. Each group was stored for 5 days at ∼16 °C, and the following parameters were evaluated: sperm metabolism of pure and diluted semen (day 1), sperm quality parameters (days 1, 3, 5), thermal-resistance test (TRT) and incubation with uterine fluid (UF) (day 5). Sperm metabolic rates between accumulative ejaculate fractions from pure and diluted semen did not show differences. Also, sperm quality parameters were not affected by the ejaculate fraction during storage. However, sperm subjected to TRT showed similar results except for progressive motility, which was better in F2 and F3 than F1. When sperm were incubated with UF, the quality decreased in each group, but sperm from F2 and F3 were less affected than those from F1. In conclusion, the post-spermatic fraction can be included in seminal doses for their use in AI-centers, with functionality of sperm of different SP origins not being impaired throughout the storage, and responding better to thermal and UF stress. However, further research in AI-centers is necessary to test the sperm behaviour under presented conditions.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
2025-09-25T12:21:40Z
2025-09-25T12:21:40Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23941
https://www.nature.com/articles/s41598-023-42254-3
2045-2322
https://doi.org/10.1038/s41598-023-42254-3
url http://hdl.handle.net/20.500.12123/23941
https://www.nature.com/articles/s41598-023-42254-3
https://doi.org/10.1038/s41598-023-42254-3
identifier_str_mv 2045-2322
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv Scientific Reports 13 : Article number: 15258 (2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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