Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina

Autores
Oriani, Alejandra Soledad; Marfil, Maria Jimena; Zumarraga, Martin Jose; Baldini, Mónica Diana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.
Instituto de Biotecnología
Fil: Oriani, Alejandra Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Baldini, Mónica Diana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fuente
International journal of mycobacteriology 8 (2): 138‑145. (14-Jun-2019)
Materia
Drinking Water Systems
Isolation Techniques
Red de Agua Potable
Técnicas de Aislamiento
Mycobacterium
Identification Methods
Nontuberculous Mycobacteria
Métodos de identificación
Bahía Blanca, Buenos Aires
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6200
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oai_identifier_str oai:localhost:20.500.12123/6200
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network_name_str INTA Digital (INTA)
spelling Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, ArgentinaOriani, Alejandra SoledadMarfil, Maria JimenaZumarraga, Martin JoseBaldini, Mónica DianaDrinking Water SystemsIsolation TechniquesRed de Agua PotableTécnicas de AislamientoMycobacteriumIdentification MethodsNontuberculous MycobacteriaMétodos de identificaciónBahía Blanca, Buenos AiresBackground: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.Instituto de BiotecnologíaFil: Oriani, Alejandra Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Baldini, Mónica Diana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaWolters Kluwer; Asian African Society of Mycobacteriology2019-10-24T18:06:01Z2019-10-24T18:06:01Z2019-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Orianihttp://hdl.handle.net/20.500.12123/62002212-5531https://doi.org/10.4103/ijmy.ijmy_39_19International journal of mycobacteriology 8 (2): 138‑145. (14-Jun-2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengBuenos Aires (province)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:07Zoai:localhost:20.500.12123/6200instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:07.468INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
title Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
spellingShingle Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
Oriani, Alejandra Soledad
Drinking Water Systems
Isolation Techniques
Red de Agua Potable
Técnicas de Aislamiento
Mycobacterium
Identification Methods
Nontuberculous Mycobacteria
Métodos de identificación
Bahía Blanca, Buenos Aires
title_short Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
title_full Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
title_fullStr Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
title_full_unstemmed Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
title_sort Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina
dc.creator.none.fl_str_mv Oriani, Alejandra Soledad
Marfil, Maria Jimena
Zumarraga, Martin Jose
Baldini, Mónica Diana
author Oriani, Alejandra Soledad
author_facet Oriani, Alejandra Soledad
Marfil, Maria Jimena
Zumarraga, Martin Jose
Baldini, Mónica Diana
author_role author
author2 Marfil, Maria Jimena
Zumarraga, Martin Jose
Baldini, Mónica Diana
author2_role author
author
author
dc.subject.none.fl_str_mv Drinking Water Systems
Isolation Techniques
Red de Agua Potable
Técnicas de Aislamiento
Mycobacterium
Identification Methods
Nontuberculous Mycobacteria
Métodos de identificación
Bahía Blanca, Buenos Aires
topic Drinking Water Systems
Isolation Techniques
Red de Agua Potable
Técnicas de Aislamiento
Mycobacterium
Identification Methods
Nontuberculous Mycobacteria
Métodos de identificación
Bahía Blanca, Buenos Aires
dc.description.none.fl_txt_mv Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.
Instituto de Biotecnología
Fil: Oriani, Alejandra Soledad. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Baldini, Mónica Diana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
description Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahía Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarity <99%. Conclusions: It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-24T18:06:01Z
2019-10-24T18:06:01Z
2019-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani
http://hdl.handle.net/20.500.12123/6200
2212-5531
https://doi.org/10.4103/ijmy.ijmy_39_19
url http://www.ijmyco.org/article.asp?issn=2212-5531;year=2019;volume=8;issue=2;spage=138;epage=145;aulast=Oriani
http://hdl.handle.net/20.500.12123/6200
https://doi.org/10.4103/ijmy.ijmy_39_19
identifier_str_mv 2212-5531
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Buenos Aires (province)
dc.publisher.none.fl_str_mv Wolters Kluwer; Asian African Society of Mycobacteriology
publisher.none.fl_str_mv Wolters Kluwer; Asian African Society of Mycobacteriology
dc.source.none.fl_str_mv International journal of mycobacteriology 8 (2): 138‑145. (14-Jun-2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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