Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis

Autores
Frare, Romina Alejandra; Stritzler, Margarita; Gomez, Maria Cristina; Tajima, Hiromi; Pascuan, Cecilia Gabriela; López Fernández, María Paula; Bottero, Ana Emilia; Nikel, Pablo I.; Alleva, Karina Edith; Ayub, Nicolás Daniel; Blumwald, Eduardo; Soto, Gabriela Cynthia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities of NOD26 are thought to be important for nodule development, osmotic balance, and ammonia efflux from the symbiosome. However, the widely accepted relevance of NOD26 in nitrogen-fixing symbiosis has never been explored in planta. Recently, we have reported the emergence of NOD26 in the nitrogen-fixing clade of angiosperms via tandem duplication. Here, we characterized the two copies of NOD26 from Medicago truncatula (Medtr8g087710 and Medtr8g087720) in their transport abilities, and at gene expression and genetic levels. Similar to their homologous soybean gene, MtNOD26 genes encode water and ammonia transport activities in heterologous expression systems. By using multiple transcriptional studies (RT-qPCR, transcriptional fusion and RNA-Seq analyses), we found that the expression of MtNOD26 copies is restricted to the nodule and gradually increases from the bacteria-free meristematic region to the nitrogen-fixation zone. Under nitrogen-limiting soil conditions, the homozygous insertional mutant lines of these two MtNOD26 genes had the same aberrant nodulation phenotype and chlorosis. Similar to uninoculated wild-type plants, inoculated mutants were unable to grow in minimal medium without a nitrogen source. Using the CRISPR/Cas9 system, we have edited the orthologous NOD26 genes in Medicago sativa (alfalfa), generating plants with aberrant nodules, chlorosis and impaired grow under nitrogen-limiting conditions. Collectively, our findings suggest functional equivalence between NOD26 copies and underline a crucial role of NOD26 in symbiotic nitrogen fixation.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tajima, Hiromi. University of California. Department of Plant Sciences; Estados Unidos
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López Fernández, María Paula. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Bottero, Ana Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nikel, Pablo I. Technical University of Denmark. The Novo Nordisk Foundation Center for Biosustainability; Dinamarca
Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant Cell, Tissue and Organ Culture (Published: 27 July 2022)
Materia
Symbiosis
Legumes
Rhizobiaceae
Ammonia
CRISPR
Simbiosis
Leguminosas
Amoníaco
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12639
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12639
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosisFrare, Romina AlejandraStritzler, MargaritaGomez, Maria CristinaTajima, HiromiPascuan, Cecilia GabrielaLópez Fernández, María PaulaBottero, Ana EmiliaNikel, Pablo I.Alleva, Karina EdithAyub, Nicolás DanielBlumwald, EduardoSoto, Gabriela CynthiaSymbiosisLegumesRhizobiaceaeAmmoniaCRISPRSimbiosisLeguminosasAmoníacoRepeticiones Palindrómicas Cortas Agrupadas y Regularmente InterespaciadasThe multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities of NOD26 are thought to be important for nodule development, osmotic balance, and ammonia efflux from the symbiosome. However, the widely accepted relevance of NOD26 in nitrogen-fixing symbiosis has never been explored in planta. Recently, we have reported the emergence of NOD26 in the nitrogen-fixing clade of angiosperms via tandem duplication. Here, we characterized the two copies of NOD26 from Medicago truncatula (Medtr8g087710 and Medtr8g087720) in their transport abilities, and at gene expression and genetic levels. Similar to their homologous soybean gene, MtNOD26 genes encode water and ammonia transport activities in heterologous expression systems. By using multiple transcriptional studies (RT-qPCR, transcriptional fusion and RNA-Seq analyses), we found that the expression of MtNOD26 copies is restricted to the nodule and gradually increases from the bacteria-free meristematic region to the nitrogen-fixation zone. Under nitrogen-limiting soil conditions, the homozygous insertional mutant lines of these two MtNOD26 genes had the same aberrant nodulation phenotype and chlorosis. Similar to uninoculated wild-type plants, inoculated mutants were unable to grow in minimal medium without a nitrogen source. Using the CRISPR/Cas9 system, we have edited the orthologous NOD26 genes in Medicago sativa (alfalfa), generating plants with aberrant nodules, chlorosis and impaired grow under nitrogen-limiting conditions. Collectively, our findings suggest functional equivalence between NOD26 copies and underline a crucial role of NOD26 in symbiotic nitrogen fixation.Instituto de BiotecnologíaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tajima, Hiromi. University of California. Department of Plant Sciences; Estados UnidosFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: López Fernández, María Paula. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Bottero, Ana Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nikel, Pablo I. Technical University of Denmark. The Novo Nordisk Foundation Center for Biosustainability; DinamarcaFil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados UnidosFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; ArgentinaFil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2022-08-22T10:16:18Z2022-08-22T10:16:18Z2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12639https://link.springer.com/article/10.1007/s11240-022-02357-71573-5044https://doi.org/10.1007/s11240-022-02357-7Plant Cell, Tissue and Organ Culture (Published: 27 July 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuarioinfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:41Zoai:localhost:20.500.12123/12639instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:41.957INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
title Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
spellingShingle Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
Frare, Romina Alejandra
Symbiosis
Legumes
Rhizobiaceae
Ammonia
CRISPR
Simbiosis
Leguminosas
Amoníaco
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
title_short Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
title_full Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
title_fullStr Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
title_full_unstemmed Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
title_sort Retrotransposon and CRISPR/Cas9‑mediated knockout of NOD26 impairs the legume‑rhizobia symbiosis
dc.creator.none.fl_str_mv Frare, Romina Alejandra
Stritzler, Margarita
Gomez, Maria Cristina
Tajima, Hiromi
Pascuan, Cecilia Gabriela
López Fernández, María Paula
Bottero, Ana Emilia
Nikel, Pablo I.
Alleva, Karina Edith
Ayub, Nicolás Daniel
Blumwald, Eduardo
Soto, Gabriela Cynthia
author Frare, Romina Alejandra
author_facet Frare, Romina Alejandra
Stritzler, Margarita
Gomez, Maria Cristina
Tajima, Hiromi
Pascuan, Cecilia Gabriela
López Fernández, María Paula
Bottero, Ana Emilia
Nikel, Pablo I.
Alleva, Karina Edith
Ayub, Nicolás Daniel
Blumwald, Eduardo
Soto, Gabriela Cynthia
author_role author
author2 Stritzler, Margarita
Gomez, Maria Cristina
Tajima, Hiromi
Pascuan, Cecilia Gabriela
López Fernández, María Paula
Bottero, Ana Emilia
Nikel, Pablo I.
Alleva, Karina Edith
Ayub, Nicolás Daniel
Blumwald, Eduardo
Soto, Gabriela Cynthia
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Symbiosis
Legumes
Rhizobiaceae
Ammonia
CRISPR
Simbiosis
Leguminosas
Amoníaco
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
topic Symbiosis
Legumes
Rhizobiaceae
Ammonia
CRISPR
Simbiosis
Leguminosas
Amoníaco
Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas
dc.description.none.fl_txt_mv The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities of NOD26 are thought to be important for nodule development, osmotic balance, and ammonia efflux from the symbiosome. However, the widely accepted relevance of NOD26 in nitrogen-fixing symbiosis has never been explored in planta. Recently, we have reported the emergence of NOD26 in the nitrogen-fixing clade of angiosperms via tandem duplication. Here, we characterized the two copies of NOD26 from Medicago truncatula (Medtr8g087710 and Medtr8g087720) in their transport abilities, and at gene expression and genetic levels. Similar to their homologous soybean gene, MtNOD26 genes encode water and ammonia transport activities in heterologous expression systems. By using multiple transcriptional studies (RT-qPCR, transcriptional fusion and RNA-Seq analyses), we found that the expression of MtNOD26 copies is restricted to the nodule and gradually increases from the bacteria-free meristematic region to the nitrogen-fixation zone. Under nitrogen-limiting soil conditions, the homozygous insertional mutant lines of these two MtNOD26 genes had the same aberrant nodulation phenotype and chlorosis. Similar to uninoculated wild-type plants, inoculated mutants were unable to grow in minimal medium without a nitrogen source. Using the CRISPR/Cas9 system, we have edited the orthologous NOD26 genes in Medicago sativa (alfalfa), generating plants with aberrant nodules, chlorosis and impaired grow under nitrogen-limiting conditions. Collectively, our findings suggest functional equivalence between NOD26 copies and underline a crucial role of NOD26 in symbiotic nitrogen fixation.
Instituto de Biotecnología
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Frare, Romina Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Frare, Romina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Stritzler, Margarita. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Stritzler, Margarita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gomez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Gomez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tajima, Hiromi. University of California. Department of Plant Sciences; Estados Unidos
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Pascuan, Cecilia Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López Fernández, María Paula. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bottero, Ana Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Bottero, Ana Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nikel, Pablo I. Technical University of Denmark. The Novo Nordisk Foundation Center for Biosustainability; Dinamarca
Fil: Alleva, Karina Edith. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Fisicoquímica Biológica; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Ayub, Nicolás Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blumwald, Eduardo. University of California. Department of Plant Sciences; Estados Unidos
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Soto, Gabriela Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética Ewald A. Favret; Argentina
Fil: Soto, Gabriela Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The multifunctional channel NOD26, identified and extensively studied (both biochemically and biophysically) in soybean, is a major protein component of the symbiosome membrane. The water and ammonia transport activities of NOD26 are thought to be important for nodule development, osmotic balance, and ammonia efflux from the symbiosome. However, the widely accepted relevance of NOD26 in nitrogen-fixing symbiosis has never been explored in planta. Recently, we have reported the emergence of NOD26 in the nitrogen-fixing clade of angiosperms via tandem duplication. Here, we characterized the two copies of NOD26 from Medicago truncatula (Medtr8g087710 and Medtr8g087720) in their transport abilities, and at gene expression and genetic levels. Similar to their homologous soybean gene, MtNOD26 genes encode water and ammonia transport activities in heterologous expression systems. By using multiple transcriptional studies (RT-qPCR, transcriptional fusion and RNA-Seq analyses), we found that the expression of MtNOD26 copies is restricted to the nodule and gradually increases from the bacteria-free meristematic region to the nitrogen-fixation zone. Under nitrogen-limiting soil conditions, the homozygous insertional mutant lines of these two MtNOD26 genes had the same aberrant nodulation phenotype and chlorosis. Similar to uninoculated wild-type plants, inoculated mutants were unable to grow in minimal medium without a nitrogen source. Using the CRISPR/Cas9 system, we have edited the orthologous NOD26 genes in Medicago sativa (alfalfa), generating plants with aberrant nodules, chlorosis and impaired grow under nitrogen-limiting conditions. Collectively, our findings suggest functional equivalence between NOD26 copies and underline a crucial role of NOD26 in symbiotic nitrogen fixation.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-22T10:16:18Z
2022-08-22T10:16:18Z
2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12639
https://link.springer.com/article/10.1007/s11240-022-02357-7
1573-5044
https://doi.org/10.1007/s11240-022-02357-7
url http://hdl.handle.net/20.500.12123/12639
https://link.springer.com/article/10.1007/s11240-022-02357-7
https://doi.org/10.1007/s11240-022-02357-7
identifier_str_mv 1573-5044
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I115-001/2019-PE-E6-I115-001/AR./Edición génica, transgénesis y mutagénesis como generadores de nueva variabilidad en especies de interés agropecuario
info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001/2019-PD-E6-I116-001/AR./Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Cell, Tissue and Organ Culture (Published: 27 July 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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