Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets

Autores
Lencina, Florencia Aylen; Olivero, Carolina Raquel; Zimmermann, Jorge Alberto; Stegmayer, María Angeles; Sirini, Noelí Estefanía; Frizzo, Laureano Sebastián; Soto, Lorena Paola; Signorini Porchiett, Marcelo Lisandro; Zbrun, Maria Virginia
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from cecal content (n = 270), neck skin (n = 270), and wastewater (n = 9), and at retail markets from breast skin (n = 241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/mL) and identified as C. jejuni or C. coli by PCR. The agar microdilution test was used to determine the minimum inhibitory concentration for ciprofloxacin and erythromycin, and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter spp. resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug resistant and harbored key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter spp. in the later stages of the poultry meat supply chain.
EEA Rafaela
Fil: Lencina, Florencia Aylen. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Olivero, Carolina Raquel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Olivero, Carolina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Stegmayer, María Angeles. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Stegmayer, María Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Sirini, Noelí Estefanía. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Sirini, Noelí Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Zbrun, Maria Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fuente
Antibiotics 15 (2) : 158. (February 2026)
Materia
Campylobacter
Resistance to Antibiotics
Abattoirs
Retail Markets
Broiler Chickens
Virulence
Resistencia a los Antibióticos
Mataderos
Mercado Minorista
Pollo de Engorde
Virulencia
Slaughterhouses
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25189
Acceder
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oai_identifier_str oai:localhost:20.500.12123/25189
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spelling Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail MarketsLencina, Florencia AylenOlivero, Carolina RaquelZimmermann, Jorge AlbertoStegmayer, María AngelesSirini, Noelí EstefaníaFrizzo, Laureano SebastiánSoto, Lorena PaolaSignorini Porchiett, Marcelo LisandroZbrun, Maria VirginiaCampylobacterResistance to AntibioticsAbattoirsRetail MarketsBroiler ChickensVirulenceResistencia a los AntibióticosMataderosMercado MinoristaPollo de EngordeVirulenciaSlaughterhousesBackground: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from cecal content (n = 270), neck skin (n = 270), and wastewater (n = 9), and at retail markets from breast skin (n = 241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/mL) and identified as C. jejuni or C. coli by PCR. The agar microdilution test was used to determine the minimum inhibitory concentration for ciprofloxacin and erythromycin, and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter spp. resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug resistant and harbored key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter spp. in the later stages of the poultry meat supply chain.EEA RafaelaFil: Lencina, Florencia Aylen. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Olivero, Carolina Raquel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Olivero, Carolina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Stegmayer, María Angeles. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Stegmayer, María Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Sirini, Noelí Estefanía. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Sirini, Noelí Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Frizzo, Laureano Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; ArgentinaFil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Zbrun, Maria Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; ArgentinaFil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaMDPI2026-02-12T13:09:07Z2026-02-12T13:09:07Z2026-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25189https://www.mdpi.com/2079-6382/15/2/1582079-6382https://doi.org/10.3390/antibiotics15020158Antibiotics 15 (2) : 158. (February 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:47:42Zoai:localhost:20.500.12123/25189instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:47:42.526INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
title Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
spellingShingle Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
Lencina, Florencia Aylen
Campylobacter
Resistance to Antibiotics
Abattoirs
Retail Markets
Broiler Chickens
Virulence
Resistencia a los Antibióticos
Mataderos
Mercado Minorista
Pollo de Engorde
Virulencia
Slaughterhouses
title_short Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
title_full Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
title_fullStr Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
title_full_unstemmed Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
title_sort Detection and Characterization of Thermotolerant Campylobacter Resistant to Antibiotics of Priority Use in Humans Present in Broiler Slaughterhouses and Retail Markets
dc.creator.none.fl_str_mv Lencina, Florencia Aylen
Olivero, Carolina Raquel
Zimmermann, Jorge Alberto
Stegmayer, María Angeles
Sirini, Noelí Estefanía
Frizzo, Laureano Sebastián
Soto, Lorena Paola
Signorini Porchiett, Marcelo Lisandro
Zbrun, Maria Virginia
author Lencina, Florencia Aylen
author_facet Lencina, Florencia Aylen
Olivero, Carolina Raquel
Zimmermann, Jorge Alberto
Stegmayer, María Angeles
Sirini, Noelí Estefanía
Frizzo, Laureano Sebastián
Soto, Lorena Paola
Signorini Porchiett, Marcelo Lisandro
Zbrun, Maria Virginia
author_role author
author2 Olivero, Carolina Raquel
Zimmermann, Jorge Alberto
Stegmayer, María Angeles
Sirini, Noelí Estefanía
Frizzo, Laureano Sebastián
Soto, Lorena Paola
Signorini Porchiett, Marcelo Lisandro
Zbrun, Maria Virginia
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Campylobacter
Resistance to Antibiotics
Abattoirs
Retail Markets
Broiler Chickens
Virulence
Resistencia a los Antibióticos
Mataderos
Mercado Minorista
Pollo de Engorde
Virulencia
Slaughterhouses
topic Campylobacter
Resistance to Antibiotics
Abattoirs
Retail Markets
Broiler Chickens
Virulence
Resistencia a los Antibióticos
Mataderos
Mercado Minorista
Pollo de Engorde
Virulencia
Slaughterhouses
dc.description.none.fl_txt_mv Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from cecal content (n = 270), neck skin (n = 270), and wastewater (n = 9), and at retail markets from breast skin (n = 241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/mL) and identified as C. jejuni or C. coli by PCR. The agar microdilution test was used to determine the minimum inhibitory concentration for ciprofloxacin and erythromycin, and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter spp. resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug resistant and harbored key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter spp. in the later stages of the poultry meat supply chain.
EEA Rafaela
Fil: Lencina, Florencia Aylen. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Olivero, Carolina Raquel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Olivero, Carolina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Stegmayer, María Angeles. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Stegmayer, María Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Sirini, Noelí Estefanía. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Sirini, Noelí Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. (ICiVet-Litoral). Laboratorio de Análisis de Alimentos; Argentina
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Zbrun, Maria Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina
Fil: Zbrun, Maria Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Zbrun, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
description Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from cecal content (n = 270), neck skin (n = 270), and wastewater (n = 9), and at retail markets from breast skin (n = 241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/mL) and identified as C. jejuni or C. coli by PCR. The agar microdilution test was used to determine the minimum inhibitory concentration for ciprofloxacin and erythromycin, and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter spp. resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug resistant and harbored key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter spp. in the later stages of the poultry meat supply chain.
publishDate 2026
dc.date.none.fl_str_mv 2026-02-12T13:09:07Z
2026-02-12T13:09:07Z
2026-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25189
https://www.mdpi.com/2079-6382/15/2/158
2079-6382
https://doi.org/10.3390/antibiotics15020158
url http://hdl.handle.net/20.500.12123/25189
https://www.mdpi.com/2079-6382/15/2/158
https://doi.org/10.3390/antibiotics15020158
identifier_str_mv 2079-6382
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Antibiotics 15 (2) : 158. (February 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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