Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets

Autores
Lencina, Florencia Aylen; Zbrun, María Virginia
Año de publicación
2026
Idioma
español castellano
Tipo de recurso
conjunto de datos
Estado
Descripción
Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain.
Fil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/278567
Acceder
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spelling Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail marketsLencina, Florencia AylenZbrun, María Virginiahttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain.Fil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina2026info:ar-repo/semantics/conjuntoDeDatosv1.0info:eu-repo/semantics/dataSetapplication/vnd.openxmlformats-officedocument.spreadsheetml.sheethttp://hdl.handle.net/11336/278567Lencina, Florencia Aylen; Zbrun, María Virginia; (2026): Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/278567CONICET DigitalCONICETspainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2026-02-26T10:27:55Zoai:ri.conicet.gov.ar:11336/278567instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982026-02-26 10:27:55.768CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
title Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
spellingShingle Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
Lencina, Florencia Aylen
title_short Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
title_full Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
title_fullStr Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
title_full_unstemmed Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
title_sort Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets
dc.creator.none.fl_str_mv Lencina, Florencia Aylen
Zbrun, María Virginia
author Lencina, Florencia Aylen
author_facet Lencina, Florencia Aylen
Zbrun, María Virginia
author_role author
author2 Zbrun, María Virginia
author2_role author
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain.
Fil: Lencina, Florencia Aylen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Investigacion de la Cadena Lactea. - Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela. Instituto de Investigacion de la Cadena Lactea.; Argentina
description Background: This study aimed to assess the presence of thermotolerant Campylobacter resistant to ciprofloxacin and erythromycin in poultry slaughterhouses and retail markets, as well as to characterize their multidrug resistance profiles, genetic determinants, and clonal relationships. Methods: Samples were collected at slaughterhouses from caecal content (n= 270), neck skin (n= 270), and wastewater (n= 9) and at retail markets from breast skin (n=241). Isolates were obtained from mCCDA agar supplemented with ciprofloxacin (2 μg/ml) and identified as C. jejuni or C. coli by PCR. Agar microdilution test was used to determine the minimum inhibitory concentration for both ciprofloxacin and erythromycin and other critical antibiotics. Point mutations in gyrA (Thr86Ile) and 23S rRNA (A2075G), virulence genes (flaA, flhA, cadF, and cdt), and clonal relationships were assessed by PCR and PFGE. Results: At the slaughterhouses, thermotolerant Campylobacter resistant to erythromycin and ciprofloxacin were detected in 48.55% (107/549) of the samples, whereas 4.56% (11/241) of retail samples were positive. The Thr86Ile substitution in gyrA and the A2075G mutation in the 23S rRNA gene were detected in 92.97% and 89.84% of the isolates, respectively. Most isolates (>80%) were multidrug-resistant, harboured key virulence genes (flaA, flhA, and cadF). C. jejuni exhibited the highest prevalence of cdt genes (76.19%). There was substantial genotypic diversity among isolates, with broad distribution across the sampled matrices and sites. Conclusions: These findings highlight the circulation of multidrug-resistant and potentially virulent thermotolerant Campylobacter in the later stages of the poultry meat supply chain.
publishDate 2026
dc.date.none.fl_str_mv 2026
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dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/278567
Lencina, Florencia Aylen; Zbrun, María Virginia; (2026): Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/278567
CONICET Digital
CONICET
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identifier_str_mv Lencina, Florencia Aylen; Zbrun, María Virginia; (2026): Detection and characterization of thermotolerant Campylobacter resistant to antibiotics of priority use in humans present in broiler slaughterhouses and retail markets. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/278567
CONICET Digital
CONICET
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