A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates

Autores
Maidana, Silvina Soledad; Miño, Samuel; Apostolo, Romina Maria; De Stefano, Gabriel Alejandro; Romera, Sonia Alejandra
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
Instituto de Virología
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Apostolo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Esquel. Grupo de Producción Animal; Argentina
Fil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina. Universidad de Morón. Facultad de Ciencias exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina
Fuente
Journal of Veterinary Diagnostic Investigation 32 (1) : 112-117 (Febrero 2020)
Materia
Bovine Herpesvirus
Genetic Techniques
Genetic Isolation
Herpes Virus Bovino
PCR
Técnica Genética
Aislamiento Genético
BoHV-1
PCR Múltiple
Nuevo Método Molecular
Multiplex PCR
New Molecular Method
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7868
Acceder
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spelling A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolatesMaidana, Silvina SoledadMiño, SamuelApostolo, Romina MariaDe Stefano, Gabriel AlejandroRomera, Sonia AlejandraBovine HerpesvirusGenetic TechniquesGenetic IsolationHerpes Virus BovinoPCRTécnica GenéticaAislamiento GenéticoBoHV-1PCR MúltipleNuevo Método MolecularMultiplex PCRNew Molecular MethodBovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.Instituto de VirologíaFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Apostolo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Esquel. Grupo de Producción Animal; ArgentinaFil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina. Universidad de Morón. Facultad de Ciencias exactas, Químicas y Naturales. Cátedra de Inmunogenética; ArgentinaSage Publications2020-09-10T16:40:56Z2020-09-10T16:40:56Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7868https://journals.sagepub.com/doi/abs/10.1177/10406387198986921943-49361040-6387https://doi.org/10.1177/1040638719898692Journal of Veterinary Diagnostic Investigation 32 (1) : 112-117 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.info:eu-repograntAgreement/INTA/PNSA/1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.info:eu-repo/semantics/restrictedAccess2025-10-16T09:29:53Zoai:localhost:20.500.12123/7868instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:53.898INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
title A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
spellingShingle A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
Maidana, Silvina Soledad
Bovine Herpesvirus
Genetic Techniques
Genetic Isolation
Herpes Virus Bovino
PCR
Técnica Genética
Aislamiento Genético
BoHV-1
PCR Múltiple
Nuevo Método Molecular
Multiplex PCR
New Molecular Method
title_short A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
title_full A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
title_fullStr A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
title_full_unstemmed A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
title_sort A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates
dc.creator.none.fl_str_mv Maidana, Silvina Soledad
Miño, Samuel
Apostolo, Romina Maria
De Stefano, Gabriel Alejandro
Romera, Sonia Alejandra
author Maidana, Silvina Soledad
author_facet Maidana, Silvina Soledad
Miño, Samuel
Apostolo, Romina Maria
De Stefano, Gabriel Alejandro
Romera, Sonia Alejandra
author_role author
author2 Miño, Samuel
Apostolo, Romina Maria
De Stefano, Gabriel Alejandro
Romera, Sonia Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine Herpesvirus
Genetic Techniques
Genetic Isolation
Herpes Virus Bovino
PCR
Técnica Genética
Aislamiento Genético
BoHV-1
PCR Múltiple
Nuevo Método Molecular
Multiplex PCR
New Molecular Method
topic Bovine Herpesvirus
Genetic Techniques
Genetic Isolation
Herpes Virus Bovino
PCR
Técnica Genética
Aislamiento Genético
BoHV-1
PCR Múltiple
Nuevo Método Molecular
Multiplex PCR
New Molecular Method
dc.description.none.fl_txt_mv Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
Instituto de Virología
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Apostolo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Esquel. Grupo de Producción Animal; Argentina
Fil: De Stefano, Gabriel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina. Universidad de Morón. Facultad de Ciencias exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina
description Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-10T16:40:56Z
2020-09-10T16:40:56Z
2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7868
https://journals.sagepub.com/doi/abs/10.1177/1040638719898692
1943-4936
1040-6387
https://doi.org/10.1177/1040638719898692
url http://hdl.handle.net/20.500.12123/7868
https://journals.sagepub.com/doi/abs/10.1177/1040638719898692
https://doi.org/10.1177/1040638719898692
identifier_str_mv 1943-4936
1040-6387
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNSA/1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche.
info:eu-repograntAgreement/INTA/PNSA/1115055/AR./Enfermedades infecciosas, parasitarias y toxico metabólicas que afectan la productividad de los ovinos, caprinos y camélidos.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage Publications
publisher.none.fl_str_mv Sage Publications
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 32 (1) : 112-117 (Febrero 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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