Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings

Autores
Colque Caro, Luis Adrián; Barbeito, Claudio Gustavo; Moore, Prando Dadin; Micheloud, Juan Francisco
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Experimental y Comparada. Laboratorio de Histología y Embriología Descriptiva; Argentina
Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT La Plata; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Moore, Prando Dadin. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina
Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fuente
Research in Veterinary Science 197 : 105926. (December 2025)
Materia
Caprinos
Enfermedades de los Animales
Hipotiroidismo
Bocio
Histomorfología
Goats
Animal Diseases
Hypothyroidism
Goitre
Histomorphology
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24523
Acceder
id INTADig_cf7bbda6215279a5fb5ff8ef7601b9d6
oai_identifier_str oai:localhost:20.500.12123/24523
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findingsColque Caro, Luis AdriánBarbeito, Claudio GustavoMoore, Prando DadinMicheloud, Juan FranciscoCaprinosEnfermedades de los AnimalesHipotiroidismoBocioHistomorfologíaGoatsAnimal DiseasesHypothyroidismGoitreHistomorphologyThe study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants.Instituto de Investigación Animal del Chaco SemiáridoFil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; ArgentinaFil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Experimental y Comparada. Laboratorio de Histología y Embriología Descriptiva; ArgentinaFil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT La Plata; ArgentinaFil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Moore, Prando Dadin. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; ArgentinaFil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; ArgentinaFil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaElsevier2025-11-10T13:22:59Z2025-11-10T13:22:59Z2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24523https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X1532-26610034-5288https://doi.org/10.1016/j.rvsc.2025.105926Research in Veterinary Science 197 : 105926. (December 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-27T08:40:52Zoai:localhost:20.500.12123/24523instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:40:53.026INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
title Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
spellingShingle Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
Colque Caro, Luis Adrián
Caprinos
Enfermedades de los Animales
Hipotiroidismo
Bocio
Histomorfología
Goats
Animal Diseases
Hypothyroidism
Goitre
Histomorphology
title_short Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
title_full Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
title_fullStr Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
title_full_unstemmed Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
title_sort Experimental induction of hypothyroidism and goitre in goat kids: Clinical, pathological and histomorphometric findings
dc.creator.none.fl_str_mv Colque Caro, Luis Adrián
Barbeito, Claudio Gustavo
Moore, Prando Dadin
Micheloud, Juan Francisco
author Colque Caro, Luis Adrián
author_facet Colque Caro, Luis Adrián
Barbeito, Claudio Gustavo
Moore, Prando Dadin
Micheloud, Juan Francisco
author_role author
author2 Barbeito, Claudio Gustavo
Moore, Prando Dadin
Micheloud, Juan Francisco
author2_role author
author
author
dc.subject.none.fl_str_mv Caprinos
Enfermedades de los Animales
Hipotiroidismo
Bocio
Histomorfología
Goats
Animal Diseases
Hypothyroidism
Goitre
Histomorphology
topic Caprinos
Enfermedades de los Animales
Hipotiroidismo
Bocio
Histomorfología
Goats
Animal Diseases
Hypothyroidism
Goitre
Histomorphology
dc.description.none.fl_txt_mv The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants.
Instituto de Investigación Animal del Chaco Semiárido
Fil: Colque Caro, Luis Adrián. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Colque Caro, Luis Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina
Fil: Barbeito, Claudio G. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Experimental y Comparada. Laboratorio de Histología y Embriología Descriptiva; Argentina
Fil: Barbeito, Claudio G. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT La Plata; Argentina
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Moore, Prando Dadin. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Sanidad Animal; Argentina
Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. CCT Salta - Jujuy; Argentina
Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina
description The study of hypothyroidism and experimentally induced goitre in young goats enables the characterization of hormonal dynamics and histological alterations in the thyroid gland. The objective of this study was to characterize histopathological and histomorphometric changes in the thyroid glands (TG) of goat kids following experimental induction of goitre using 6-n-propyl-2-thiouracil (PTU). Eighteen healthy lactating Saanen goat kids (35 days old; 6.2 kg body weight) were randomly assigned to three groups: control (basal diet +3 ml fresh milk), low-dose PTU (3.5 mg/kg BW/day in 3 ml milk), and high-dose PTU (7 mg/kg BW/day in 3 ml milk). The study spanned 116 days, during which participants underwent weekly body weight monitoring and PTU dose adjustments. At the conclusion of the trial, necropsies were conducted on 16 animals, and their TGs were evaluated both macroscopically and microscopically. Blood samples were collected on a biweekly basis to assess circulating thyroid hormone levels. No significant differences in weight gain were observed during the first 56 days; however, from day 56 onward, children in the high-dose PTU group exhibited reduced average daily gain compared to the low-dose and control groups. Thyroid gland weights exhibited an increase in both treated groups (up to 10.5 μg), accounting for 0.04 % and 0.03 % of body weight in the low- and high-dose groups, respectively, compared to 0.01 % in the control group. A histopathological evaluation revealed marked follicular hyperplasia and hypertrophy, with significant alterations in follicular morphology, epithelial height, and interfollicular spacing. Hormonal profiles revealed dose-dependent disruptions in T3, T4, and T3/T4 ratios, with the most pronounced alterations observed in high-dose PTU-treated animals. Chronic administration of PTU (propylthiouracil) led to significant structural and functional alterations in the thyroid gland, validating the experimental model's suitability for investigating iodine deficiency–related goitre in young ruminants.
publishDate 2025
dc.date.none.fl_str_mv 2025-11-10T13:22:59Z
2025-11-10T13:22:59Z
2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24523
https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X
1532-2661
0034-5288
https://doi.org/10.1016/j.rvsc.2025.105926
url http://hdl.handle.net/20.500.12123/24523
https://www.sciencedirect.com/science/article/abs/pii/S003452882500400X
https://doi.org/10.1016/j.rvsc.2025.105926
identifier_str_mv 1532-2661
0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 197 : 105926. (December 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1849949347857301504
score 13.011256

Publicaciones similares