Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis...

Autores
Ferreira, Flavia Vanina; Rivadeneira, Maria Fernanda; Bello, Fernando; Giudici, Vanesa Noelia; Musumeci, Matías Alejandro
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits. RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality. CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea.
EEA Concordia
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Giudici, Vanesa. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fuente
Pest Management Science : 1-15. (First published: 11 June 2025)
Materia
Arándano
Control Biológico
Botrytis cinerea
Agentes de Control Biológico
Blueberries
Burkholderiaceae
Biological Control
Biological Control Agents
Burkholderia contaminans
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22657
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oai_identifier_str oai:localhost:20.500.12123/22657
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spelling Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinereaFerreira, Flavia VaninaRivadeneira, Maria FernandaBello, FernandoGiudici, Vanesa NoeliaMusumeci, Matías AlejandroArándanoControl BiológicoBotrytis cinereaAgentes de Control BiológicoBlueberriesBurkholderiaceaeBiological ControlBiological Control AgentsBurkholderia contaminansBACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits. RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality. CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea.EEA ConcordiaFil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; ArgentinaFil: Giudici, Vanesa. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaFil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; ArgentinaFil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; ArgentinaWiley2025-06-12T12:40:25Z2025-06-12T12:40:25Z2025-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22657https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.89641526-498X1526-4998https://doi.org/10.1002/ps.8964Pest Management Science : 1-15. (First published: 11 June 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:20Zoai:localhost:20.500.12123/22657instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:20.954INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
title Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
spellingShingle Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
Ferreira, Flavia Vanina
Arándano
Control Biológico
Botrytis cinerea
Agentes de Control Biológico
Blueberries
Burkholderiaceae
Biological Control
Biological Control Agents
Burkholderia contaminans
title_short Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
title_full Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
title_fullStr Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
title_full_unstemmed Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
title_sort Burkholderia contaminans isolated from root of blueberry shrub produces occidiofungins and pyrrolnitrin useful to prevent postharvest decays of blueberry fruits caused by Botrytis cinerea
dc.creator.none.fl_str_mv Ferreira, Flavia Vanina
Rivadeneira, Maria Fernanda
Bello, Fernando
Giudici, Vanesa Noelia
Musumeci, Matías Alejandro
author Ferreira, Flavia Vanina
author_facet Ferreira, Flavia Vanina
Rivadeneira, Maria Fernanda
Bello, Fernando
Giudici, Vanesa Noelia
Musumeci, Matías Alejandro
author_role author
author2 Rivadeneira, Maria Fernanda
Bello, Fernando
Giudici, Vanesa Noelia
Musumeci, Matías Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arándano
Control Biológico
Botrytis cinerea
Agentes de Control Biológico
Blueberries
Burkholderiaceae
Biological Control
Biological Control Agents
Burkholderia contaminans
topic Arándano
Control Biológico
Botrytis cinerea
Agentes de Control Biológico
Blueberries
Burkholderiaceae
Biological Control
Biological Control Agents
Burkholderia contaminans
dc.description.none.fl_txt_mv BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits. RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality. CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea.
EEA Concordia
Fil: Ferreira, Flavia Vanina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Rivadeneira, Maria Fernanda. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Bello, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina
Fil: Giudici, Vanesa. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
Fil: Musumeci, Matías Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencia y Tecnología de los Alimentos de Entre Ríos; Argentina
Fil: Musumeci, Matías Alejandro. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Alimentación; Argentina
description BACKGROUND: Botrytis cinerea is a relevant fungal pathogen in the production of blueberries, which is managed mainly with fungicides. However, the intensive usage has led to the emergence of resistant strains. The use of microbial biocontrol agents is a sustainable strategy to overcome limitations associated with fungicides. The objectives of this work were to know the capability of the endophytic bacterium Burkholderia contaminans 128 to inhibit different phytopathogens in vitro and to prevent B. cinerea rot in blueberry fruits. RESULTS: Burkholderia contaminans 128 able to grow on filaments of Trichoderma citrinoviride was isolated from roots of blueberry shrub. The bacterial cell-free supernatant (Bc128 CFS) inhibited efficiently the mycelia growth and conidia germination of B. cinerea and other important pathogens affecting blueberry such as Alternaria sp., Colletotrichum gloeosporioides and Fusarium graminearum. HPLC–MS/MS analyses of Bc128 CFS confirmed production of occidiofungins and pyrrolnitrin, compounds with fungicide activity but different inhibition mechanisms. Qualitative and quantitative HPLC-MS/MS analyses determined the presence of indole-3-acetic acid in Bc128 CFS, a phytohormone involved in plant-growth promotion. Bc128 CFS inhibited completely the natural occurrence of conidia of Alternaria sp., Colletotrichum sp. and Pestalotiopsis sp. and reduced significantly the incidence of B. cinerea conidia in blueberry flowers. Bc128 CFS decreased the virulence of B. cinerea in blueberries approximately 90%. Bc128 CFS was more efficient to control B. cinerea in preventive than curative treatments and did not affect fruit quality. CONCLUSION: B. contaminans 128 provides direct and indirect antibiosis mechanisms useful to prevent rots in blueberries caused by B. cinerea.
publishDate 2025
dc.date.none.fl_str_mv 2025-06-12T12:40:25Z
2025-06-12T12:40:25Z
2025-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22657
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8964
1526-498X
1526-4998
https://doi.org/10.1002/ps.8964
url http://hdl.handle.net/20.500.12123/22657
https://scijournals.onlinelibrary.wiley.com/doi/10.1002/ps.8964
https://doi.org/10.1002/ps.8964
identifier_str_mv 1526-498X
1526-4998
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Pest Management Science : 1-15. (First published: 11 June 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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