Genetic variability within accessions of the B73 maize inbred line

Autores
Olmos, Sofí­a Eugenia; Lorea, Roberto Daniel; Eyherabide, Guillermo
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The maize inbred line B73 has been extensively studied at the molecular level. Researchers now have access to the genome sequence of B73 as well as to databases of biallelic and multiallelic markers where functional polymorphisms between B73 and a public inbred line population can be compared to relate genotypes to phenotypes. This indicates the importance to determine the genetic fidelity of the germplasm during preservation and propagation processes, in particular, when seeds of reference inbred lines such as B73 are maintained. The aim of this study was to assess the genetic uniformity among three different sources of the B73 inbred line by means of 75 Simple Sequence Repeats (SSRs). The three B73 sources showed homozygosis; however, some accessions differed greatly from the expected locus size predicted at the reference B73 genomic sequence. A particular haplotype was prevalent in the USDA accession PI550473. The error rate of the allele size determination was estimated. The genotyping technique used in this work allowed the separation of alleles of ± 2 bp range difference within the same electrophoresis run, whereas allele size estimations between experiments, within the laboratory, differed in ± 4 bp range difference. Besides experimental errors in genotyping, the putative cause of differences among accessions could be attributed to seed contamination and genetic drift. The B73 accessions evaluated in our work can be shared among laboratories to precise genotyping and phenotyping of maize inbred lines.
Fil: Olmos, Sofí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Lorea, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Eyherabide, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fuente
Maydica 59 (3-4) : 298-305. (2014)
Materia
Zea Mays
Maize
Genetic Variation
Plant Breeding
Genetic Markers
Inbred Lines
Variación Genética
Fitomejoramiento
Microsatélites
Microsatellites
Marcadores Genéticos
Lineas Consanguíneas
Fidelidad Genética
B73
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1136
Acceder
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spelling Genetic variability within accessions of the B73 maize inbred lineOlmos, Sofí­a EugeniaLorea, Roberto DanielEyherabide, GuillermoZea MaysMaizeGenetic VariationPlant BreedingGenetic MarkersInbred LinesVariación GenéticaFitomejoramientoMicrosatélitesMicrosatellitesMarcadores GenéticosLineas ConsanguíneasFidelidad GenéticaB73The maize inbred line B73 has been extensively studied at the molecular level. Researchers now have access to the genome sequence of B73 as well as to databases of biallelic and multiallelic markers where functional polymorphisms between B73 and a public inbred line population can be compared to relate genotypes to phenotypes. This indicates the importance to determine the genetic fidelity of the germplasm during preservation and propagation processes, in particular, when seeds of reference inbred lines such as B73 are maintained. The aim of this study was to assess the genetic uniformity among three different sources of the B73 inbred line by means of 75 Simple Sequence Repeats (SSRs). The three B73 sources showed homozygosis; however, some accessions differed greatly from the expected locus size predicted at the reference B73 genomic sequence. A particular haplotype was prevalent in the USDA accession PI550473. The error rate of the allele size determination was estimated. The genotyping technique used in this work allowed the separation of alleles of ± 2 bp range difference within the same electrophoresis run, whereas allele size estimations between experiments, within the laboratory, differed in ± 4 bp range difference. Besides experimental errors in genotyping, the putative cause of differences among accessions could be attributed to seed contamination and genetic drift. The B73 accessions evaluated in our work can be shared among laboratories to precise genotyping and phenotyping of maize inbred lines.Fil: Olmos, Sofí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; ArgentinaFil: Lorea, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; ArgentinaFil: Eyherabide, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina2017-09-05T18:02:44Z2017-09-05T18:02:44Z2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1136http://www.maydica.org/articles/59_298.pdf2279-8013Maydica 59 (3-4) : 298-305. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:46:58Zoai:localhost:20.500.12123/1136instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:00.199INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic variability within accessions of the B73 maize inbred line
title Genetic variability within accessions of the B73 maize inbred line
spellingShingle Genetic variability within accessions of the B73 maize inbred line
Olmos, Sofí­a Eugenia
Zea Mays
Maize
Genetic Variation
Plant Breeding
Genetic Markers
Inbred Lines
Variación Genética
Fitomejoramiento
Microsatélites
Microsatellites
Marcadores Genéticos
Lineas Consanguíneas
Fidelidad Genética
B73
title_short Genetic variability within accessions of the B73 maize inbred line
title_full Genetic variability within accessions of the B73 maize inbred line
title_fullStr Genetic variability within accessions of the B73 maize inbred line
title_full_unstemmed Genetic variability within accessions of the B73 maize inbred line
title_sort Genetic variability within accessions of the B73 maize inbred line
dc.creator.none.fl_str_mv Olmos, Sofí­a Eugenia
Lorea, Roberto Daniel
Eyherabide, Guillermo
author Olmos, Sofí­a Eugenia
author_facet Olmos, Sofí­a Eugenia
Lorea, Roberto Daniel
Eyherabide, Guillermo
author_role author
author2 Lorea, Roberto Daniel
Eyherabide, Guillermo
author2_role author
author
dc.subject.none.fl_str_mv Zea Mays
Maize
Genetic Variation
Plant Breeding
Genetic Markers
Inbred Lines
Variación Genética
Fitomejoramiento
Microsatélites
Microsatellites
Marcadores Genéticos
Lineas Consanguíneas
Fidelidad Genética
B73
topic Zea Mays
Maize
Genetic Variation
Plant Breeding
Genetic Markers
Inbred Lines
Variación Genética
Fitomejoramiento
Microsatélites
Microsatellites
Marcadores Genéticos
Lineas Consanguíneas
Fidelidad Genética
B73
dc.description.none.fl_txt_mv The maize inbred line B73 has been extensively studied at the molecular level. Researchers now have access to the genome sequence of B73 as well as to databases of biallelic and multiallelic markers where functional polymorphisms between B73 and a public inbred line population can be compared to relate genotypes to phenotypes. This indicates the importance to determine the genetic fidelity of the germplasm during preservation and propagation processes, in particular, when seeds of reference inbred lines such as B73 are maintained. The aim of this study was to assess the genetic uniformity among three different sources of the B73 inbred line by means of 75 Simple Sequence Repeats (SSRs). The three B73 sources showed homozygosis; however, some accessions differed greatly from the expected locus size predicted at the reference B73 genomic sequence. A particular haplotype was prevalent in the USDA accession PI550473. The error rate of the allele size determination was estimated. The genotyping technique used in this work allowed the separation of alleles of ± 2 bp range difference within the same electrophoresis run, whereas allele size estimations between experiments, within the laboratory, differed in ± 4 bp range difference. Besides experimental errors in genotyping, the putative cause of differences among accessions could be attributed to seed contamination and genetic drift. The B73 accessions evaluated in our work can be shared among laboratories to precise genotyping and phenotyping of maize inbred lines.
Fil: Olmos, Sofí­a Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Lorea, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
Fil: Eyherabide, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina
description The maize inbred line B73 has been extensively studied at the molecular level. Researchers now have access to the genome sequence of B73 as well as to databases of biallelic and multiallelic markers where functional polymorphisms between B73 and a public inbred line population can be compared to relate genotypes to phenotypes. This indicates the importance to determine the genetic fidelity of the germplasm during preservation and propagation processes, in particular, when seeds of reference inbred lines such as B73 are maintained. The aim of this study was to assess the genetic uniformity among three different sources of the B73 inbred line by means of 75 Simple Sequence Repeats (SSRs). The three B73 sources showed homozygosis; however, some accessions differed greatly from the expected locus size predicted at the reference B73 genomic sequence. A particular haplotype was prevalent in the USDA accession PI550473. The error rate of the allele size determination was estimated. The genotyping technique used in this work allowed the separation of alleles of ± 2 bp range difference within the same electrophoresis run, whereas allele size estimations between experiments, within the laboratory, differed in ± 4 bp range difference. Besides experimental errors in genotyping, the putative cause of differences among accessions could be attributed to seed contamination and genetic drift. The B73 accessions evaluated in our work can be shared among laboratories to precise genotyping and phenotyping of maize inbred lines.
publishDate 2014
dc.date.none.fl_str_mv 2014
2017-09-05T18:02:44Z
2017-09-05T18:02:44Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1136
http://www.maydica.org/articles/59_298.pdf
2279-8013
url http://hdl.handle.net/20.500.12123/1136
http://www.maydica.org/articles/59_298.pdf
identifier_str_mv 2279-8013
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Maydica 59 (3-4) : 298-305. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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