ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus

Autores
Vega, Celina Guadalupe; Garaicoechea, Lorena Laura; Degiuseppe, Juan Ignacio; Bok, Marina; Rivolta, Alejandra Antonella; Piantanida, Ana Paula; Asenzo, Gustavo Daniel; Aduriz Guerrero, Matías; Wigdorovitz, Andres; Stupka, Juan A.; Parreño, Gladys Viviana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide.
Instituto de Virología
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Vega, Celina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Degiuseppe, Juan Ignacio. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Piantanida, Ana Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Asenzo, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Asenzo, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aduriz Guerrero, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Aduriz Guerrero, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stupka, Juan A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Virological Methods 298 : 114279 (December 2021)
Materia
Nanobiotechnology
Rotavirus
ELISA
Diagnosis
Nanobiotecnología
Diagnóstico
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26518
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network_name_str INTA Digital (INTA)
spelling ROTADIAL : The first nanobody-based immunoassay to detect Group A RotavirusVega, Celina GuadalupeGaraicoechea, Lorena LauraDegiuseppe, Juan IgnacioBok, MarinaRivolta, Alejandra AntonellaPiantanida, Ana PaulaAsenzo, Gustavo DanielAduriz Guerrero, MatíasWigdorovitz, AndresStupka, Juan A.Parreño, Gladys VivianaNanobiotechnologyRotavirusELISADiagnosisNanobiotecnologíaDiagnósticoROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide.Instituto de VirologíaFil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Vega, Celina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; ArgentinaFil: Degiuseppe, Juan Ignacio. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; ArgentinaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; ArgentinaFil: Piantanida, Ana Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; ArgentinaFil: Asenzo, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Asenzo, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Aduriz Guerrero, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Aduriz Guerrero, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stupka, Juan A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2026-06-08T09:55:59Z2026-06-08T09:55:59Z2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26518https://www.sciencedirect.com/science/article/pii/S01660934210021841879-09840166-0934https://doi.org/10.1016/j.jviromet.2021.114279Journal of Virological Methods 298 : 114279 (December 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-06-18T09:34:28Zoai:localhost:20.500.12123/26518instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-06-18 09:34:29.167INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
title ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
spellingShingle ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
Vega, Celina Guadalupe
Nanobiotechnology
Rotavirus
ELISA
Diagnosis
Nanobiotecnología
Diagnóstico
title_short ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
title_full ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
title_fullStr ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
title_full_unstemmed ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
title_sort ROTADIAL : The first nanobody-based immunoassay to detect Group A Rotavirus
dc.creator.none.fl_str_mv Vega, Celina Guadalupe
Garaicoechea, Lorena Laura
Degiuseppe, Juan Ignacio
Bok, Marina
Rivolta, Alejandra Antonella
Piantanida, Ana Paula
Asenzo, Gustavo Daniel
Aduriz Guerrero, Matías
Wigdorovitz, Andres
Stupka, Juan A.
Parreño, Gladys Viviana
author Vega, Celina Guadalupe
author_facet Vega, Celina Guadalupe
Garaicoechea, Lorena Laura
Degiuseppe, Juan Ignacio
Bok, Marina
Rivolta, Alejandra Antonella
Piantanida, Ana Paula
Asenzo, Gustavo Daniel
Aduriz Guerrero, Matías
Wigdorovitz, Andres
Stupka, Juan A.
Parreño, Gladys Viviana
author_role author
author2 Garaicoechea, Lorena Laura
Degiuseppe, Juan Ignacio
Bok, Marina
Rivolta, Alejandra Antonella
Piantanida, Ana Paula
Asenzo, Gustavo Daniel
Aduriz Guerrero, Matías
Wigdorovitz, Andres
Stupka, Juan A.
Parreño, Gladys Viviana
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanobiotechnology
Rotavirus
ELISA
Diagnosis
Nanobiotecnología
Diagnóstico
topic Nanobiotechnology
Rotavirus
ELISA
Diagnosis
Nanobiotecnología
Diagnóstico
dc.description.none.fl_txt_mv ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide.
Instituto de Virología
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Vega, Celina Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Garaicoechea, Lorena Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Degiuseppe, Juan Ignacio. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivolta, Alejandra Antonella. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Piantanida, Ana Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA; Argentina
Fil: Asenzo, Gustavo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Asenzo, Gustavo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aduriz Guerrero, Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Aduriz Guerrero, Matías. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Wigdorovitz, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stupka, Juan A. Instituto Nacional de Enfermedades Infecciosas -ANLIS "Dr​ Carlos.G. Malbrán". Laboratorio de Laboratorio de Gastroenteritis Virales; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description ROTADIAL is a rapid nanobody (Nb)-based ELISA assay able to identify Rotavirus group A (RVA) in feces from pediatric patients. The assay is based on a sandwich of two patented llama-derived Nbs directed to the inner capsid viral protein VP6 from RVA. Nbs are directed to conformational epitopes of VP6 and recognized all human RVA strains tested, representing ideal reagents for their use in immunodiagnostic tests for RVA detection. All the steps are carried out at room temperature, bringing results in less than two hours. This assay, named ROTADIAL, was validated with a reference panel of feces from pediatric patients from Argentina. The overall sensitivity and specificity of the ROTADIAL test, when compared to a commercial test, was 100 % (100/100) and 99 % (99/100) respectively. ROTADIAL presented optimal analytical performance, being capable of detecting RVA regardless of the presence of other common human enteric infectious agents and is the first RVA-diagnostic assay developed using Nbs, worldwide.
publishDate 2021
dc.date.none.fl_str_mv 2021-12
2026-06-08T09:55:59Z
2026-06-08T09:55:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26518
https://www.sciencedirect.com/science/article/pii/S0166093421002184
1879-0984
0166-0934
https://doi.org/10.1016/j.jviromet.2021.114279
url http://hdl.handle.net/20.500.12123/26518
https://www.sciencedirect.com/science/article/pii/S0166093421002184
https://doi.org/10.1016/j.jviromet.2021.114279
identifier_str_mv 1879-0984
0166-0934
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Virological Methods 298 : 114279 (December 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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